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The single-cell transcriptome data of pig embryonic stem cells cultured without feeder cells

ABSTRACT: The single-cell transcriptome data of pig embryonic stem cells cultured without feeder cells

PROVIDER: PRJNA781835 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16980914.fastq.gz	Fastqsanger.gz
	SRR16980915.fastq.gz	Fastqsanger.gz
	SRR16980916.fastq.gz	Fastqsanger.gz
	SRR16980917.fastq.gz	Fastqsanger.gz
	SRR16980918.fastq.gz	Fastqsanger.gz

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The transcriptome data of pig embryonic stem cells cultured without feeder cells

Project description:Here, we examined the effect of LDN-193189, an inhibitor of the BMP signaling pathway, on pig embryonic stem cells (ESCs) in the absence of feeder cells to develop a feeder-free culture system. Feeder cells play an important role in supporting the pluripotency of pig ESCs by blocking trophoblast and mesodermal differentiation through inhibition of the BMP pathway. Transcriptome analysis revealed that LDN-193189 treatment instead of coculturing with feeder cells could help maintain the pluripotency and homogenous cell population of pig ESCs by stimulating the secretion of chemokines and suppressing differentiation. We confirmed that LDN-193189-treated pig ESCs cultured without feeder cells maintained pluripotency for an extended period and were capable of differentiating in vivo and in vitro. The pig ESCs represented distinct characteristics of pig epiblasts as an in vivo counterpart at a transcriptomic level. In summary, we developed feeder-free culture systems for pig ESCs, and our findings will improve the understanding of pluripotency gene networks and comparative embryogenesis.

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