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PRC2-mediated repression is essential to maintain identity and function of differentiated dopaminergic and serotonergic neurons [snRNA-Seq]

ABSTRACT: PRC2-mediated repression is essential to maintain identity and function of differentiated dopaminergic and serotonergic neurons [snRNA-Seq]

PROVIDER: PRJNA782566 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17002488.fastq.gz	Fastqsanger.gz
	SRR17002489.fastq.gz	Fastqsanger.gz
	SRR17002490.fastq.gz	Fastqsanger.gz
	SRR17002491.fastq.gz	Fastqsanger.gz
	SRR17002492.fastq.gz	Fastqsanger.gz

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PRC2-mediated repression is essential to maintain identity and function of differentiated dopaminergic and serotonergic neurons [snRNA-Seq]

Project description:Neurons of the CNS must maintain their distinct identity over an entire lifespan. Apart from instructive information provided by transcription factors driving neuron specific genes, other gene programs need to be permanently silenced. The mechanisms governing enduring gene-silencing in neurons are largely unknown, as are the consequences if they fail. Here we show that loss of the Polycomb repressive complex 2 (PRC2) obligate unit Eed in differentiated murine midbrain dopamine (mDA) neurons resulted in progressive loss of the H3K27me3 histone modification followed by upregulation of PRC2 targets, also highly enriched for the constitutive heterochromatin H3K9me3 modification. This was followed by reduced expression of mDA neuron-identity genes, particularly evident in the Substantia nigra pars compacta. Consequently, mDA-neuronal function was severely disrupted, causing parkinsonian-like motor skill deficits. Deletion of Eed in differentiated serotonergic (5HT) neurons resulted in a similar upregulation of PRC2-targets and reduced expression of genes defining 5HT neurons, which promoted loss of serotonergic identity followed by loss of function and altered behaviour. Overall, our results reveal that PRC2 inactivation leads to a selective and progressive loss of mDA or 5HT neuronal identity and function, but not to any loss of cells. Thus, our study shows that PRC2-dependent maintenance of neuronal identity is essential and safeguards against expression of other non-relevant gene programs and loss of neuronal function.

2022-07-11 | GSE189329 | GEO

PRC2-mediated repression is essential to maintain identity and function of differentiated dopaminergic and serotonergic neurons

Project description:PRC2-mediated repression is essential to maintain identity and function of differentiated dopaminergic and serotonergic neurons

| PRJNA781198 | ENA

PRC2-mediated repression is essential to maintain identity and function of differentiated dopaminergic and serotonergic neurons [ChIP-Seq]

2022-07-11 | GSE189017 | GEO

PRC2-mediated repression is essential to maintain identity and function of differentiated dopaminergic and serotonergic neurons [bulk RNA-Seq]

2022-07-11 | GSE189328 | GEO

PRC2-mediated repression is essential to maintain identity and function of differentiated dopaminergic and serotonergic neurons [ChIP-Seq]

Project description:PRC2-mediated repression is essential to maintain identity and function of differentiated dopaminergic and serotonergic neurons [ChIP-Seq]

| PRJNA781201 | ENA

PRC2-mediated repression is essential to maintain identity and function of differentiated dopaminergic and serotonergic neurons [bulk RNA-Seq]

Project description:PRC2-mediated repression is essential to maintain identity and function of differentiated dopaminergic and serotonergic neurons [bulk RNA-Seq]

| PRJNA782568 | ENA

The divergent nodes of non-autonomous UPRER signaling through serotonergic and dopaminergic neurons

Project description:Performed RNA-seq analysis of animals with xbp-1s overexpression (ER stress response transcription factor) in specific neuron types: pan-neuronal, serotonergic neuron, dopaminergic neuron, and both serotonergic and dopaminergic neurons, all compared to a wild-type control. RNA-seq was performed on purified RNA extracted from ~1000 whole worms using a proprietary Genewiz protocol described briefly in the manuscript. 3 biological replicates are provided for each sample.

2021-01-01 | E-MTAB-9771 | biostudies-arrayexpress

PRC2-mediated repression is essential to maintain identity and function of differentiated dopaminergic and serotonergic neurons.

Project description:How neurons can maintain cellular identity over an entire life span remains largely unknown. Here, we show that maintenance of identity in differentiated dopaminergic and serotonergic neurons is critically reliant on the Polycomb repressive complex 2 (PRC2). Deletion of the obligate PRC2 component, Eed, in these neurons resulted in global loss of H3K27me3, followed by a gradual activation of genes harboring both H3K27me3 and H3K9me3 modifications. Notably, H3K9me3 was lost at these PRC2 targets before gene activation. Neuronal survival was not compromised; instead, there was a reduction in subtype-specific gene expression and a progressive impairment of dopaminergic and serotonergic neuronal function, leading to behavioral deficits characteristic of Parkinson's disease and anxiety. Single-cell analysis revealed subtype-specific vulnerability to loss of PRC2 repression in dopamine neurons of the substantia nigra. Our study reveals that a PRC2-dependent nonpermissive chromatin state is essential to maintain the subtype identity and function of dopaminergic and serotonergic neurons.

| S-EPMC9417181 | biostudies-literature

The divergent nodes of non-autonomous UPRER signaling through serotonergic and dopaminergic neurons

Project description:The divergent nodes of non-autonomous UPRER signaling through serotonergic and dopaminergic neurons

| PRJEB41394 | ENA

A comprehensive map coupling histone modifications with gene regulation in adult dopaminergic and serotonergic neurons [scRNA-Seq]

Project description:The adult brain is composed of hundreds of different neuronal subtypes, which retain their differentiated traits and identity throughout the lifespan of the organism. Nevertheless, in part due to technical limitations, the mechanisms governing this stability are not fully understood. Here, using a strategy that allows for ChIP-seq combined with RNA-seq in sparse neuronal populations in vivo, we present, to our knowledge, the first comparative analysis of permissive and repressive histone modifications in adult midbrain dopaminergic neurons, raphe nuclei serotonergic neurons and embryonic neural progenitors. Our results support a model wherein a sequential deposition of the repressive modifications H3K27me3 and H3K9me3 occur on developmental genes in a neuronal subtype specific manner. We furthermore show that aberrant gene expression during dopaminergic stress in a mouse model of Parkinson’s disease, or after methamphetamine injection, is characterized by the de-repression of genes with promoter regions that are dually marked by H3K4me3 and H3K27me3, whereas the induction of genes with promoter regions marked by any other combination of H3K27me3 and H3K9me3 occur less frequently. Our study provides to our knowledge the first genome-wide analysis of permissive/repressive histone modifications coupled to gene expression in these rare but clinically relevant neuronal subtypes. This strategy can be generalized for the identification and functional characterization of molecular determinants involved in the maintenance of gene expression in other classes of neurons.

2018-01-25 | GSE107740 | GEO

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