Project description:We constructed three small RNA libraries from embryos at 5, 6 and 7 post-oviposition (hpo) of Bactrocera dorsalis for deep sequencing. The data analysis revealed 147 known and 103 novel miRNAs from these libraries.
Project description:H3, H3K27me3 and H3K4me3 ChIP-seq
| PRJDB11556 | ENA
Project description:RNA-seq Danzhou, Guangzhou, Wuhan and Nanyang Population of Bactrocera dorsalis reared at 25, 17 and 32 Celsius Thorax muscles and Head
| PRJNA1037329 | ENA
Project description:miRNA-seq Danzhou, Guangzhou, Wuhan and Nanyang Population of Bactrocera dorsalis reared at 25, 17 and 32 Celsius Thorax muscles and Head
Project description:We developed a ChIP protocol for the analysis of histone marks using less than 10,000 cells per IP, and used it to investigate the chromatin state of E11.5 mouse primordial germ cells (PGCs). A genome-wide ChIP-Seq analysis of E11.5 PGCs revealed a distribution of H3K4me3/H3K27me3 bivalent domains highly enriched for developmental regulatory genes. H3K4me3 and H3K27me3 ChIP-Seq from mouse E11.5 primordial germ cells.
Project description:Four developmental stage small RNA libraries including Eggs, Larvae, Pupae and Adults were constructed, where a lot of known miRNAs were identified and many novel miRNAs were predicted. Comparison of their expression profiles in the four libraries suggested that Bactrocera dorsalis miRNAs are dynamically regulated throughout the life cycle.Further analysis of the expression and function of these miRNAs could increase our understanding of regulatory networks in the insect and lead to novel approaches to its control.
Project description:The molecular signature at histone H3K4me3 and H3K27me3 involved in epigenetic regulation of normal (MCF10A) and transformed (MCF7, MDA-MB-231) breast cells using ChIP-Seq technology. This study examines the dynamic distribution of H3K4me3, associated with active chromatin, and H3K27me3, associated with repressed chromatin, histone modifications to provide an understanding of the changes in epigenetic regulation associated with the unique breast cancer subtypes. histone H3K4me3 and H3K27me3 ChIP-seq normal (MCF10A) and transformed (MCF7, MDA-MB-231) breast cells Please note that the 'H3K4me3' and 'input' data are duplicated records of the samples represented in GSE69377 for the convenient retrieval of the complete raw data from SRA.
