Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

SEMA3E-PLXND1 induced transcriptional changes in B16 melanocytes

ABSTRACT: SEMA3E-PLXND1 induced transcriptional changes in B16 melanocytes

PROVIDER: PRJNA783012 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

SEMA3E-PLXND1 induced transcriptional changes in B16 melanocytes

Project description:Exposure to SEMA3E cause an immediate but transient collapse in F-actin of B16 melanocytes expressing the receptor PLXND1. Concomitant signal transduction by activated PLXND1 receptor mediated via its GTPase activating domain has a role in adapting the melanocytes to its micro-environment. To analyse these adaptive transcriptional responses B16 melanocytes were treated with SEMA3E for 2h to study early responses and for 8h to study prolonged responses. Cytochalasin D (8h) was used to distinguish the effects brough about by cytoskeletal collapse alone.

2024-07-01 | GSE189438 | GEO

Melan-a melanocytes and B16 melanoma cells

Project description:This SuperSeries is composed of the following subset Series: GSE11580: Time course RA-treatment of B16 mouse melanoma cells GSE11584: Melan-a mouse melanocytes vs. B16 mouse melanoma cells Keywords: SuperSeries Refer to individual Series

2008-07-31 | E-GEOD-11588 | biostudies-arrayexpress

Melan-a mouse melanocytes vs. B16 mouse melanoma cells

Project description:Gene expression profile of melan-a mouse melanocytes vs. B16 mouse melanoma cells. Keywords: Cell type comparison

2008-07-31 | GSE11584 | GEO

Transcription profiling of mouse of melan-a mouse melanocytes vs. B16 melanoma cells

Project description:Gene expression profile of melan-a mouse melanocytes vs. B16 mouse melanoma cells. Experiment Overall Design: Balanced block design with dye swap. Six biological replicates, one replicate per array.

2008-10-21 | E-GEOD-11584 | biostudies-arrayexpress

Comprehensive analysis of pathological changes in tumor of B16 with CCND1 overexpression

Project description:Aims: CCND1 amplification was an unfavorable prognostic factor for patients with melanoma. This study aimed to investigate the molecular changes in tumor of B16 with CCND1 overexpression. Methods: High-throughput sequencing was used to identify genes that were differentially expressed in tumor between B16 controls and B16 with CCND1 overexpression. Tumors of B16 Ctrl (n=3) and B16 with CCND1 overexpression (n=3) were enrolled in this study. Results: mRNA expression of genes associated with immunity, such as CD8, Gzm, B2m, and Tap1 were decreased in tumors with CCND1 overexpression. Conclusion: CCND1 overexpression is associated with an immunosuppressive microenvironment.

2022-07-20 | GSE180327 | GEO

RNA sequencing analysis of B16-F1, B16-F1R2 and B16-F1R2L

Project description:We wanted to correlate the protein cargo of secreted exosomes with gene expression pattern in B16-F1 and B16-F1R2. For that purpose, we performed RNA sequencing analysis of B16-F1, B16-F1R2 and B16-F1R2L (Fig.1E). We identified >3000 genes significantly up-regulated and >1000 significantly down-regulated in B16-F1R2 model compared to B16-F1, using a false discovery rate (FDR) of 0.05.

2021-07-28 | GSE150221 | GEO

Understanding gene expression signatures with pigmentation changes in Melanocytes

Project description:Understanding gene expression signatures with pigmentation changes in Melanocytes

| PRJNA420095 | ENA

CBG and EAA for melanocytes protection

Project description:Aims: This study aims to assess the effect of simultaneous use of cannabigerol (CBG) and 3-O-ethyl ascorbic acid (EAA) on changes in the skin melanocyte proteome induced by UVA radiation, with particular emphasis on modifications in the structure of metabolically important proteins. Results: Proteomic analysis allowed to identify 1248 proteins with statistically significantly changed expression following melanocytes irradiation and/or incubation with CBG/EAA. The top 25 proteins with most strongly modified expression included mainly proteins involved in cells protection/antioxidant response, as well as pro-inflammatory and proapoptotic signalization. Moreover, in melanocytes irradiated with UVA, the levels of lipid peroxidation product, 4-hydroxynonenal (4-HNE) and its protein adducts increase, as well as significant changes in the profile of proteins modified by 4-HNE are observed. CBG and EAA, especially when used together, largely reverse this effect. Innovation: This study for the first time shows the combined effect of CBG and EAA on the proteome of melanocytes after their exposure to UVA radiation, that applies to both changes in protein expression and intracellular signaling based on protein modification by lipid peroxidation products. Conclusion: It can be summarized that CBG and EAA can provide melanocytes with the most effective protection against oxidative stress, and perhaps even protect the skin structure against carcinogenesis.

2026-06-15 | PXD074276 | Pride

Transcriptional Profile in Zebrafish Melanocytes and Melanoma Tumors [RNA-seq]

Project description:With high genetic heterogeneity in melanoma, understanding epigenetic and transcriptional differences between melanocytes and melanoma cells will enable further understanding of the genes, pathways, and epigenetic regions influencing melanoma development. We performed RNA-seq and ATAC-seq on fluorescently-isolated melanocytes and melanoma cells from a zebrafish melanoma model.

2022-05-24 | GSE178801 | GEO

Gene expression changes in human melanoma cell lines compared to primary melanocytes

Project description:Gene expression changes in 3 human melanoma cell lines were compared to freshly isolated normal primary melanocytes

2017-12-21 | GSE88741 | GEO