Project description:Bacteria assume distinct lifestyles during the planktonic and biofilm modes of growth. In biofilms, they are more tolerant to antibiotics and can evade the immune system response more effectively. However, little is known regarding the molecular determinants involved in biofilm formation by Gardnerella vaginali, the predominant species found in bacterial vaginosis (BV). Hence, to gain insight into the pathogenesis of G. vaginalis, we carried out a comparative transcriptomic analysis between planktonic and biofilm phenotypes, using RNA-sequencing. The major alterations observed were related with the transcription of genes involved in cell wall biogenesis and typical stress factors, in which was found significantly up-regulated in biofilms, resulting in a protected mode of bacterial growth. In addition, biofilm phenotype was characterized by low metabolic activity, which is appropriate to guarantee long term survival during BV recurrence.
Project description:Bacterial vaginosis (BV) treatment failures or recurrences are common. To identify features associated with treatment response, we compared vaginal microbiota and host ectocervical transcriptome before and after oral metronidazole therapy. Response to metronidazole is characterized by significant changes in chemokines and related transcripts suggesting that strategies to promote these pathways may prove beneficial.
Project description:This study includes 1146 samples of host genotyping data (genotyped) from Illumina Omni arrays. Samples were collected from adults (>16 yrs) patients with CSF confirmed bacterial meningitis in the Netherlands between 2006 and 2015. Metadata includes patient outcome, species of bacteria, and for 467 samples a link to an ENA run with the associated bacterial genome (S. pneumoniae only).
Project description:The aim of the study was to investigate gene expression profiles of post-menopausal women receiving Premarin estrogen replacement therapy (ERT), compared to controls and to examine any correlations between the bacterial vaginosis (BV) status of the stubjects. This is the first study to use gene arrays to correlate changes in host expression to ERT and BV.
Project description:The sexually transmitted parasite Trichomonas vaginalis is often found in symbiosis with the obligate intracellular pathogen Mycoplasma hominis. M. hominis is itself an opportunistic pathogen of the female reproductive tract associated with bacterial vaginosis. The goal of this experiment was to identify the effects of each pathogen individually and in symbiosis on host cell gene expression.
