Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single nucleus analysis of post-mortem brain tissues from Nasu-Hakola disease (NHD) patients

ABSTRACT: Single nucleus analysis of post-mortem brain tissues from Nasu-Hakola disease (NHD) patients

PROVIDER: PRJNA785585 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR17097681_1.fastq.gz	Fastqsanger.gz
	SRR17097681_2.fastq.gz	Fastqsanger.gz
	SRR17097682_1.fastq.gz	Fastqsanger.gz
	SRR17097682_2.fastq.gz	Fastqsanger.gz
	SRR17097683_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 38

Similar Datasets

RNA-sequencing of human post-mortem brain tissues

Project description:RNA-seq profiling was conducted on clinically-annotated human post-mortem brain tissues

2017-06-22 | GSE80655 | GEO

Single Nucleus Sequencing Reveals Artifactual Gene Expression and Variance in Post-Mortem Brain

Project description:Single nucleus sequencing of fresh frozen post-mortem brain

2020-12-01 | GSE157760 | GEO

Single nucleus analysis of post-mortem brain tissues from Adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP) patients

Project description:Microglia, the brain's resident macrophages, depend on interleukin 34 (IL-34) and colony-stimulating factor 1 (CSF1) for their development and maintenance, engaging the CSF1 receptor (CSF1R). Adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP), a neurodegenerative disorder affecting the brain's white matter, is caused by heterozygous pathogenic mutations in the CSF1R gene. Neuropathological studies of postmortem ALSP brains have shown widespread depletion of microglia, demyelination of cerebral white matter, axonal swellings, and pigmented glial cells. In this study, we investigated how individual brain cell populations respond to microglia depletion and contribute to ALSP progression. To do this, we analyzed post-mortem specimens from patients wirth ALSP using single-nucleus RNA-seq (snRNA-seq).

2025-08-13 | GSE295854 | GEO

Transcriptome analysis of six tissues obtained post mortem from sepsis patients

Project description:Transcriptome analysis of six tissues obtained post mortem from sepsis patients

| PRJNA996949 | ENA

Single Nucleus Sequencing Reveals Artifactual Gene Expression and Variance in Post-Mortem Brain

Project description:Single Nucleus Sequencing Reveals Artifactual Gene Expression and Variance in Post-Mortem Brain

| PRJNA662657 | ENA

Single-nucleus RNA-seq in the post-mortem brain in major depressive disorder

Project description:Single-nucleus RNA-seq in the post-mortem brain in major depressive disorder

| PRJNA602867 | ENA

Post-mortem gene expression from mouse brain

Project description:Post-mortem gene expression from mouse brain

| PRJNA672107 | ENA

Single-nucleus RNA-seq in the post-mortem brain in major depressive disorder

Project description:We performed high-throughput snRNA-seq using the 10X Genomics Chromium platform on archived post-mortem dorsolateral prefrontal cortex (BA9) tissue in MDD subjects who died by suicide and in control subjects to identify cell-type specific differentially expressed genes.

2020-02-25 | GSE144136 | GEO

Comparison of post-mortem tissue from brain BA10 region between schizophrenic and control patients.

Project description:Analysis of gene expression in two large schizophrenia cohorts identifies multiple changes associated with nerve terminal function. Schizophrenia is a severe psychiatric disorder with a world-wide prevalence of 1%. The pathophysiology of the illness is not understood, but is thought to have a strong genetic component with some environmental influences on aetiology. To gain further insight into disease mechanism, we used microarray technology to determine the expression of over 30 000 mRNA transcripts in post-mortem tissue from a brain region associated with the pathophysiology of the disease (Brodmann area 10: anterior prefrontal cortex) in 28 schizophrenic and 23 control patients. Post-mortem derived BA10 tissue from 28 schizophrenic and 23 control patients were compared. Age, gender, post-mortem delay and pH of brain lysates data were also captured.

2009-08-13 | E-GEOD-17612 | biostudies-arrayexpress

Single nucleus analysis of post-mortem brain tissues from Nasu-Hakola disease (NHD) patients

Project description:The TREM2-DAP12 receptor complex sustains microglia functions. Heterozygous TREM2 variants subtly impair microglia, facilitating manifestation of Alzheimer’s Disease in the elderly. Homozygous inactivating mutations of TREM2 or DAP12 cause Nasu-Hakola disease (NHD), an early-onset dementia with leukoencephalopathy, myelin loss and gliosis. Here we investigated the impact of DAP12 deficiency in microglia and collateral damage to other brain cells by single-nucleus RNA-seq in NHD patients and DAP12 loss-of-function (KΔ75) mice. KΔ75 mice showed signatures of impaired microglia activation that reverberated in mild dysfunction of other brain cells. Paradoxically, NHD microglia were activated and associated with astrocytosis, hypoxia, and neuronal loss signatures. We envision that KΔ75 signatures recapitulate an early NHD stage in which DAP12-deficient microglia fail to clear toxic products generated during brain development and homeostasis. Conversely, NHD signatures reflect a late disease stage in which accumulated toxic products cause a widespread tissue damage that elicits TREM2-DAP12-independent microgliosis, astrogliosis, hypoxia, and neuronal death. This TREM2-DAP12-independent microglia activation in NHD has bearing on potential microglia-based therapies.

2022-12-31 | GSE190013 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data