Project description:The buds at same developmental stages were collected from the Magnolia hypoleuca and Magnolia officinalis individual tree planted in the Dandong, Liaoning and Mianyang Sichuan and disposed under low temperature conditions. Transcriptome
Project description:Neurodegenerative diseases involve progressive neuronal loss associated with oxidative stress (OS) and inflammation. Given the limited efficacy of current therapies, natural compounds with multitarget neuroprotective potential are of growing interest. In this study, we investigated the neuroprotective effects of a standardized Magnolia officinalis (L.) bark extract (MOE), rich in honokiol and magnolol (29.1 ± 1.39 mg/g and 16.2 ± 0.81 mg/g, HPLC-UV DAD analysis) in rat brain cortical slices exposed to hydrogen peroxide-induced OS. MOE significantly recovered tissue viability and reduced ROS and malondialdehyde levels caused by OS. Caspase-3, -8, and -9 activation was also attenuated, suggesting a role in modulating both intrinsic and extrinsic apoptotic pathways. Shotgun proteomics using LC-HRMS/MS identified OS-induced protein expression changes reversed by MOE. Thirty-three proteins significantly altered by OS were identified, fourteen of which were rescued by MOE co-treatment. These proteins participate in several processes, including neuronal survival, OS response, and proteostasis. Bioinformatic analysis demonstrated that genes responsible for protein synthesis regulated by MOE are subjected to transcriptional regulation by factors associated with OS, including FOXO4, NRF2, and SP1. The present findings support the hypothesis that MOE exerts multitarget neuroprotective effects by modulating key proteins involved in OS responses and neuronal survival, suggesting a promising natural products-based strategy for pre-venting processes related to neurodegenerative diseases.
Project description:High-throughput small RNA sequencing were performed to identify a large number of miRNAs and their targets in mature female and male G. biloba leaves for the first time. We ascertained that the regulatory networks of the miRNAs are involved in many different primary biological processes based on potential target designations. Our study is the first to provide useful information for uncovering the regulatory networks of miRNAs in basal gymnosperm G. biloba leaves. small RNA sequencing in female and male leaves of G. biloba
Project description:High-throughput small RNA sequencing were performed to identify a large number of miRNAs and their targets in mature G. biloba ovules. Our study is the first to provide useful information for uncovering the regulatory networks of miRNAs in basal gymnosperm G. biloba ovules. small RNAÂ sequencing in ovules of G. biloba
Project description:In this study, we performed LC-QTOF-MS-based metabolomics and RNA-seq based transcriptome analysis using seven tissues of Magnolia obovata
