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Pulsatilla cernua

ABSTRACT: Anemone cernua overview

PROVIDER: PRJNA787348 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR570255_1.fastq.gz	Fastqsanger.gz
	DRR570255_2.fastq.gz	Fastqsanger.gz
	DRR570256_1.fastq.gz	Fastqsanger.gz
	DRR570256_2.fastq.gz	Fastqsanger.gz
	DRR570257_1.fastq.gz	Fastqsanger.gz

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Saponins with neuroprotective effects from the roots of Pulsatilla cernua.

Project description:Four new oleanene-type triterpenoid saponins together with six known saponins were isolated from the roots of Pulsatilla cernua and their structures were elucidated on the basis of spectroscopic data, including 2D NMR spectra and chemical evidence. Among these one of the aglycones (gypsogenin) is reported for the first time from this genus. Some of these compounds showed significant neuroprotective effects against the cytotoxicity induced by ?-amyloid(25-35) (A?(25-35)) on human neuroblastoma SH-SY5Y cells.

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An Orobanche cernua x Orobanche cumana segregating population provides insight into the regulation of germination specificity in a parasitic plant

Project description:Germination of seeds of Orobanche species requires specific chemicals exuded by host roots. A family of “divergent” KARRIKIN INSENSITIVE2 (KAI2d) genes encode proteins that recognize strigolactone (SL) class germination simulants. We explored specificity of germination stimulant detection by analyzing interspecific segregants of a cross between Orobanche cernua and O. cumana, closely related species that differ in stimulant response. O. cernua parasitizes tomato and germinates in response to the SL orobanchol, while O. cumana parasitizes sunflower and responds to dehydrocostus lactone (DCL). KAI2d genes were catalogued in parents and in segregants that showed stimulant specificity. KAI2d genes were also functionally assayed in the Arabidopsis kai2 mutant background. We identified five full-length KAI2d genes in O. cernua and eight in O. cumana. The O. cernua KAI2d2, as well as its ortholog in O. cumana, are associated with SL perception. A cluster of O. cumana KAI2d genes was genetically linked to DCL perception, although no specific receptor gene was identified by heterologous complementation. These findings support the KAI2d-mediated perception of SLs, but fall short of explaining how O. cumana perceives DCL. The ability of some O. cumana KAI2d genes to detect SLs points to the involvement of additional factors in regulating stimulant specificity.

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Phylogeography of <i>Pulsatilla cernua</i> (Ranunculaceae), a grassland species, in Japan.

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