Project description:The goal of this study is to identify Macrophage-specific L13a Knockout Transcriptomic Signatures. We treated Macrophages (L13a KO and WT) with M1/M2 cytokines , and identified L13a KO specific transcriptomic changes.
Project description:Patients with peritoneal metastasis of colorectal or high grade appendiceal origin who are candidates for cytoreductive surgery with HIPEC (hyperthermic intraperitoneal chemotherapy) will be enrolled in this study. Blood collection for measurements of plasma cell-free DNA hydroxymethylation signatures will be performed at different time points, before and after surgery, in order to determine if plasma hydroxymethylation signatures are more sensitive than conventional tumor markers in identifying clinically detectable recurrence at 1 year after surgery.
Project description:Wild type and macrophage-specific-Pparg knockout C57 mice were gavaged with CMC or DEHP for 28 or 90continuous days. At the end of the experiment, livers were harvested for RNA-seq. Data analysis revealed that DEHP induced remarkable changes of lipid metabolic genes in liver, and that loss of Pparg in hepatic macrophages significantly abrogated the elevation of gene overexpression associated with fatty acid metabolism.
Project description:Interventions: experimental group :PD-1 Knockout Engineered T Cells
Primary outcome(s): Number of participants with Adverse Events and/or Dose Limiting Toxicities as a Measure of Safety and tolerability of dose of PD-1 Knockout T cells using Common Terminology Criteria for Adverse Events (CTCAE v4.0) in patients
Study Design: historical control