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Mediator facilitates transcription initiation at most promoters via a Tail-independent mechanism

ABSTRACT: Mediator facilitates transcription initiation at most promoters via a Tail-independent mechanism

PROVIDER: PRJNA788461 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17217400_1.fastq.gz	Fastqsanger.gz
	SRR17217400_2.fastq.gz	Fastqsanger.gz
	SRR17217500_1.fastq.gz	Fastqsanger.gz
	SRR17217500_2.fastq.gz	Fastqsanger.gz
	SRR17217401_1.fastq.gz	Fastqsanger.gz

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Mediator facilitates transcription initiation at most promoters via a Tail-independent mechanism [ChEC-seq]

Project description:Mediator (MED) is a conserved factor with important roles in basal and activated transcription. Here, we investigate the genome-wide roles of yeast MED by rapid depletion of its activator-binding domain (Tail) and monitoring changes in nascent transcription. Rapid Tail depletion surprisingly reduces transcription from only a small subset of genes. At most of these Tail-dependent genes, in unperturbed conditions, MED is detected at both the UASs and promoters. In contrast, at most Tail-independent genes, we find MED primarily at promoters but not at the UASs. These results suggest that MED Tail and activator-mediated MED recruitment regulate only a small subset of genes. Further, we define three classes of genes that differ in PIC assembly pathways and the requirements for MED Tail, SAGA, TFIID and BET factors Bdf1/2. Our combined results have broad implications for the roles of MED, other coactivators, and mechanisms of transcriptional regulation at different gene classes.

2022-08-22 | GSE190774 | GEO

Mediator facilitates transcription initiation at most promoters via a Tail-independent mechanism [ChIP-seq]

2022-08-22 | GSE190776 | GEO

Mediator facilitates transcription initiation at most promoters via a Tail-independent mechanism [RNA-seq]

2022-08-22 | GSE190777 | GEO

Mediator facilitates transcription initiation at most promoters via a Tail-independent mechanism [RNA-seq]

Project description:Mediator facilitates transcription initiation at most promoters via a Tail-independent mechanism [RNA-seq]

| PRJNA788465 | ENA

Mediator facilitates transcription initiation at most promoters via a Tail-independent mechanism [ChIP-seq]

Project description:Mediator facilitates transcription initiation at most promoters via a Tail-independent mechanism [ChIP-seq]

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Mediator facilitates transcription initiation at most promoters via a Tail-independent mechanism [ChEC-seq]

Project description:Mediator facilitates transcription initiation at most promoters via a Tail-independent mechanism [ChEC-seq]

| PRJNA788454 | ENA

Yeast Mediator facilitates transcription initiation at most promoters via a Tail-independent mechanism.

Project description:Mediator (MED) is a conserved factor with important roles in basal and activated transcription. Here, we investigate the genome-wide roles of yeast MED by rapid depletion of its activator-binding domain (Tail) and monitoring changes in nascent transcription. Rapid Tail depletion surprisingly reduces transcription from only a small subset of genes. At most of these Tail-dependent genes, in unperturbed conditions, MED is detected at both the UASs and promoters. In contrast, at most Tail-independent genes, we find MED primarily at promoters but not at the UASs. These results suggest that MED Tail and activator-mediated MED recruitment regulates only a small subset of genes. Furthermore, we define three classes of genes that differ in PIC assembly pathways and the requirements for MED Tail, SAGA, TFIID, and BET factors Bdf1/2. Our combined results have broad implications for the roles of MED, other coactivators, and mechanisms of transcriptional regulation at different gene classes.

| S-EPMC9637718 | biostudies-literature

Tail and Kinase Modules Differently Regulate Core Mediator Recruitment and Function In Vivo

Project description:Mediator is a highly conserved transcriptional coactivator organized into four modules, namely Tail, Middle, Head and Kinase (CKM). Previous work suggests regulatory roles for Tail and CKM, but an integrated model for these activities is lacking. Here, we analyzed the genome-wide distribution of Mediator subunits in wild-type and mutant yeast cells in which RNA polymerase II promoter escape is blocked allowing detection of transient Mediator forms. We found that while all modules are recruited to upstream activated regions (UAS), assembly of Mediator within the pre-initiation complex is accompanied by the release of CKM. Interestingly, our data show that CKM regulates Mediator-UAS interaction rather than Mediator-promoter association. In addition, while Tail is required for Mediator recruitment to UAS, Tail-less Mediator nevertheless interacts with core promoters. Collectively, our data suggest that the essential function of Mediator is mediated by Head and Middle at core promoters, while Tail and CKM play regulatory roles.

2016-10-25 | GSE81107 | GEO

Mediator facilitates transcription initiation at most promoters via a Tail-independent mechanism

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-08-22 | GSE190778 | GEO

Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters [ChIP-Seq]

Project description:The Mediator coactivator complex is divided into four modules: head, middle, tail, and kinase. Deletion of the architectural subunit Med16 separates core Mediator (cMed), comprising the head, middle, and scaffold (Med14), from the tail. However, the direct global effects of tail/cMed disconnection are unclear. We find that rapid depletion of Med16 downregulates genes that require the SAGA complex for full expression, consistent with their reported tail dependence, but also moderately overactivates TFIID-dependent genes in a manner partly dependent on the separated tail, which remains associated with upstream activating sequences. Suppression of TBP dynamics via removal of the Mot1 ATPase partially restores normal transcriptional activity to Med16-depleted cells, suggesting that cMed/tail separation results in an imbalance in the levels of PIC formation at SAGA-requiring and TFIID-dependent genes. We suggest that the preferential regulation of SAGA-requiring genes by tailed Mediator helps maintain a proper balance of transcription between these genes and those more dependent on TFIID.

2021-07-30 | GSE171981 | GEO

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