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Global genomic and proteomic analysis of TNBC cells identifies the critical role of PELP1 in ribosome biogenesis

ABSTRACT: Global genomic and proteomic analysis of TNBC cells identifies the critical role of PELP1 in ribosome biogenesis

PROVIDER: PRJNA789562 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17248242.fastq.gz	Fastqsanger.gz
	SRR17248243.fastq.gz	Fastqsanger.gz
	SRR17248244.fastq.gz	Fastqsanger.gz
	SRR17248245.fastq.gz	Fastqsanger.gz
	SRR17248246.fastq.gz	Fastqsanger.gz

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Global genomic and proteomic analysis of TNBC cells identifies the critical role of PELP1 in ribosome biogenesis

Project description:We examined the mechanisms by which PELP1 regulates TNBC progression. MDA-MB-231 cells were stably transfected with control non-targeted shRNA or PELP1 shRNA, and total RNA was isolated for RNA-seq analysis. Our results demonstrated that PELP1 regulates the expression of Myc, E2F and mTOR targets in TNBC.

2022-03-03 | GSE191066 | GEO

Targeting PELP1 reduces endometrial cancer progression via attenuation of ribosomal biogenesis

Project description:We examined the mechanisms by which SMIP34 regulates EEC progression. EEC cells (HEC-1-A) were treated with either vehicle or SMIP34 20µM for 24 hours, and total RNA was isolated for RNA-seq analysis. Our results demonstrated that PELP1 inhibition by SMIP34 reduces the expression of genes involved in ribosome biogenesis and translation.

2024-10-16 | GSE226850 | GEO

Proteomic analysis of PELP1 (RIX1) interactome.

Project description:A mass spectrometry-based proteomic analysis of the Rix1 60S biogenesis complex through immunoprecipitation of endogenous PELP1 from HEK293T cells.

2024-12-03 | MSV000096583 | MassIVE

PELP1 ChIP-chip from MCF-7 cells

Project description:Proline-, glutamic acid-, and leucine-rich protein 1 (PELP1) is a transcriptional coactivator that has previously been shown to collaborate with nuclear receptors. Evidence from other groups suggest that PELP1 may stimulate transcription by displacing the linker histone H1 and/or by recruiting the lysine demethylase KDM1 to demethylate H3K9me2. Also known as modulator of the non-genomic activity of estrogen receptor (MNAR), PELP1 has also been implicated promoting estrogen receptor signaling from the plasma membrane. We recently identified PELP1 a factor that interacts with the macro domain of the histone variant macroH2A1. To determine if PELP1 interacts with macroH2A1 in cells, we performed PELP1 ChIP-chip from MCF-7 cells. The pattern of PELP1 bind across the genome strongly correlates with that previously determined for macroH2A1. This data combined with additional experiments allow us to conclude that macroH2A1 regulates target gene expression in part by recruiting the transcriptional coregulator PELP1. Two PELP1 ChIP-chip biological replicates from MCF-7 human breast cancer cells are included.

2014-04-24 | E-GEOD-22254 | biostudies-arrayexpress

Post-transcriptional regulation of Ribosome Biogenesis

Project description:Post-transcriptional regulation of Ribosome Biogenesis

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Targeting PELP1 reduces endometrial cancer progression via attenuation of ribosomal biogenesis

Project description:Targeting PELP1 reduces endometrial cancer progression via attenuation of ribosomal biogenesis

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PELP1 ChIP-chip from MCF-7 cells

2014-04-24 | GSE22254 | GEO

The E3 SUMO ligase Mms21 controls ribosome biogenesis

Project description:The budding yeast E3 SUMO ligase Mms21, a component of the Smc5-6 complex, regulates sister chromatid cohesion, DNA replication, and DNA repair. We identify a role for Mms21 in ribosome biogenesis. The mms21RINGD mutant exhibits reduced rRNA production, nuclear accumulation of 60S and 40S ribosomal proteins, and elevated Gcn4 translation. Genes involved in ribosome biogenesis and translation are down-regulated in the mms21RINGD mutant. Examining gene expression profile of mms21RINGD mutant compared to wild-type by RNA Seq using Ilumina sequencing

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p53 activation during ribosome biogenesis regulates normal erythroid differentiation. [expression]

Project description:The role of ribosome biogenesis in erythroid development is supported by the recognition of erythroid defects in ribosomopathies in both Diamond-Blackfan anemia and 5q- syndrome. Whether ribosome biogenesis exerts a regulatory function on normal erythroid development is still unknown. In the present study, a detailed characterization of ribosome biogenesis dynamics during human and murine erythropoiesis shows that ribosome biogenesis is abruptly interrupted by the drop of rDNA transcription and the collapse of ribosomal protein neo-synthesis. Its premature arrest by RNA polI inhibitor, CX-5461 targets the proliferation of immature erythroblasts. We also show that p53 is activated spontaneously or in response to CX-5461 concomitantly to ribosome biogenesis arrest, and drives a transcriptional program in which genes involved in cell cycle arrest, negative regulation of apoptosis and DNA damage response were upregulated. RNA polI transcriptional stress results in nucleolar disruption and activation of ATR-CHK1-p53 pathway. Our results imply that the timing of ribosome biogenesis extinction and p53 activation are crucial for erythroid development. In ribosomopathies in which ribosome availability is altered by unbalanced production of ribosomal proteins, the threshold of ribosome biogenesis down-regulation could be prematurely reached and together with pathological p53 activation prevents a normal expansion of erythroid progenitors.

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Catalytic Activity of ADAR1 Governs Ribosome Biogenesis

Project description:Catalytic Activity of ADAR1 Governs Ribosome Biogenesis

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