Project description:This project focust on proteogenomic characterization of a robust low complexity biocathode community which was enriched from, and cultivated on, the cathode of a microbial solar cell (MSC). This consortium forms a multi-cell layer thick biofilm on the poised electrode surface (+310 mV SHE) and can directly use electrical current as an electron donor to fix CO2 and reduce O2.
Project description:We investigated the impacts of transition metal oxide (TMO) lithium-ion battery cathode nanomaterial, lithium cobalt oxide (LCO), on gene expression in the larvae of a model sediment invertebrate Chironomus riparius.
Project description:<p>Understanding the extracellular electron transfer mechanisms of electroactive bacteria could help determine their potential in microbial fuel cells (MFCs) and their microbial syntrophy with redox-active minerals in natural environments. However, the mechanisms of extracellular electron transfer to electrodes by sulfate-reducing bacteria (SRB) remain underexplored. Here, we utilized double-chamber MFCs with carbon cloth electrodes to investigate the extracellular electron transfer mechanisms of Desulfovibrio vulgaris Hildenborough (DvH), a model SRB, under varying lactate and sulfate concentrations using different DvH mutants. Our MFC setup indicated that DvH can harvest electrons from lactate at the anode and transfer them to cathode, where DvH could further utilize these electrons. Patterns in current production compared to variations of electron donor/acceptor ratios in the anode and cathode suggested that attachment of DvH to the electrode and biofilm density were critical for effective electricity generation. Electron microscopy analysis of DvH biofilms indicated DvH utilized filaments that resemble pili to attach on electrodes and facilitate extracellular electron transfer from cell-to-cell and to the electrode. Proteomics profiling indicated that DvH adapted to electroactive respiration by presenting more pili- and flagellar- related proteins. The mutant with a deletion of the major pilus-producing gene yielded less voltage and far less attachment to both anodic and cathodic electrodes, suggesting the importance of pili in extracellular electron transfer. The mutant with a deficiency in biofilm formation, however, did not eliminate current production indicating the existence of indirect extracellular electron transfer. Untargeted metabolomics profiling showed flavin-based metabolites, potential electron shuttles.</p>
2024-12-06 | MTBLS11447 | MetaboLights
Project description:cathode bofilm
| PRJNA791161 | ENA
Project description:bacterial community of cathode