Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Translation rescue by targeting Ppp1r15a upstream open reading frame in vivo

ABSTRACT: Translation rescue by targeting Ppp1r15a upstream open reading frame in vivo

PROVIDER: PRJNA790822 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR17278432_1.fastq.gz	Fastqsanger.gz
	SRR17278432_2.fastq.gz	Fastqsanger.gz
	SRR17278433_1.fastq.gz	Fastqsanger.gz
	SRR17278433_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 73

Similar Datasets

Translation rescue by targeting Ppp1r15a upstream open reading frame in vivo

Project description:The eIF2 initiation complex is central to maintaining a functional translation machinery. Extreme stress such as life-threatening sepsis exposes vulnerabilities in this tightly regulated system, resulting in an imbalance between the opposing actions of kinases and phosphatases on the main regulatory subunit eIF2α. Here, we report that translation shutdown is a hallmark of established sepsis-induced kidney injury brought about by excessive eIF2α phosphorylation and sustained by blunted expression of the counterregulatory phosphatase subunit Ppp1r15a. We determined that the blunted Ppp1r15a expression persists because of the presence of an upstream open reading frame (uORF). Overcoming this barrier with genetic approaches enabled the derepression of Ppp1r15a, salvaged translation and improved kidney function in an endotoxemia model. We also found that the loss of this uORF has broad effects on the composition and phosphorylation status of the immunopeptidome that extended beyond the eIF2α axis. Collectively, our findings define the breath and potency of the highly conserved Ppp1r15a uORF and provide a paradigm for the design of uORF-based translation rheostat strategies. The ability to accurately control the dynamics of translation during sepsis will open new paths for the development of therapies at codon level precision.

2021-12-22 | GSE191262 | GEO

Translation rescue by targeting Ppp1r15a upstream open reading frame in vivo

Project description:The eIF2 initiation complex is central to maintaining a functional translation machinery. Extreme stress such as life-threatening sepsis exposes vulnerabilities in this tightly regulated system, resulting in an imbalance between the opposing actions of kinases and phosphatases on the main regulatory subunit eIF2α. Here, we report that translation shutdown is a hallmark of established sepsis-induced kidney injury brought about by excessive eIF2α phosphorylation and sustained by blunted expression of the counterregulatory phosphatase subunit Ppp1r15a. We determined that the blunted Ppp1r15a expression persists because of the presence of an upstream open reading frame (uORF). Overcoming this barrier with genetic approaches enabled the depression of Ppp1r15a, salvaged translation and improved kidney function in an endotoxemia model. We also found that the loss of this uORF has broad effects on the composition and phosphorylation status of the immunopeptidome that extended beyond the eIF2α axis. Collectively, our findings define the breadth and potency of the highly conserved Ppp1r15a uORF and provide a paradigm for the design of uORF-based translation rheostat strategies. The ability to accurately control the dynamics of translation during sepsis will open new paths for the development of therapies at codon level precision.

2022-09-28 | PXD030889 | Pride

Temperature-dependent regulation of upstream open reading frame translation in s. cerevisiae

Project description:Temperature-dependent regulation of upstream open reading frame translation in s. cerevisiae

| PRJNA564290 | ENA

Upstream open reading frame translation enhances immunogenic peptide presentation in mitotically arrested cancer cells

Project description:Upstream open reading frame translation enhances immunogenic peptide presentation in mitotically arrested cancer cells

| PRJNA1180945 | ENA

Upstream open reading frame translation enhances immunogenic peptide presentation in mitotically arrested cancer cells

Project description:Upstream open reading frame translation enhances immunogenic peptide presentation in mitotically arrested cancer cells

| PRJNA1182812 | ENA

Upstream open reading frame translation enhances immunogenic peptide presentation in mitotically arrested cancer cells

Project description:Mitosis is a critical phase of the cell cycle and a vulnerable point where cancer cells can be effectively disrupted, leading to cell death and inhibition of tumor growth. However, challenges such as drug resistance remain significant in clinical applications. During mitosis, mRNA translation is generally downregulated, while non-canonical translation of specific transcripts proceeds. Here, we demonstrate that mitotic cancer cells redistribute ribosomes toward the 5' untranslated region (5' UTR) and the start of the coding sequence (CDS), enhancing the translation of thousands of upstream open reading frames (uORFs) and upstream overlapping open reading frames (uoORFs). This mitotic induction of uORF/uoORF enriches the presentation of immunopeptides at the surface of cancer cells following treatment with mitotic inhibitors. Functional assays indicate the potential of such neoepitopes to provoke cancer-cell killing by T cells. Altogether, our findings highlight the therapeutic potential of targeting uORF/uoORF-derived neoepitopes in combination with mitotic inhibitors to enhance immune recognition and tumor cell elimination.

2025-06-30 | GSE280870 | GEO

Upstream open reading frame translation enhances immunogenic peptide presentation in mitotically arrested cancer cells

2025-04-24 | GSE281253 | GEO

Circadian ribosome profiling reveals a role for the Period2 upstream opening reading frame in sleep

Project description:Circadian ribosome profiling reveals a role for the Period2 upstream opening reading frame in sleep

| PRJNA832792 | ENA

Circadian ribosome profiling reveals a role for the Period2 upstream opening reading frame in sleep.

Project description:Circadian ribosome profiling reveals a role for the Period2 upstream opening reading frame in sleep.

| PRJNA967742 | ENA

Alternative Reading Frame expression in POLG gene

Project description:POLGARF is an upstream Alternative Reading Frame in POLG gene partially overlapping POLG sequence. To obtain POLGARF peptide reference spectra POLGARF was overexpressed in HEK293T and HEK293F cells .

2021-09-08 | PXD016007 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data