Similar Datasets
Project description:Pseudomonas aeruginosa is a multidrug-resistant opportunistic pathogen, with chronic infections often associated with biofilms that enhance antibiotic resistance. This study investigates the uncharacterized gene PA3049, which is upregulated under biofilm conditions, to determine its role in infection, biofilm formation, and antimicrobial tolerance. o Using bioinformatics, infection models, and molecular microbiology, we determined that PA3049 contributed to biofilm establishment both in vitro and in high-validity infection models. We also identified its role in bacterial survival under sub-inhibitory concentrations of antibiotics and its impact on pyocyanin production. Proteomic analysis revealed that PA3049 upregulates the R2-type pyocin cluster, which drives explosive cell lysis and extracellular DNA (eDNA) release during early stages of P. aeruginosa biofilm development. Additionally, PA3049 interacts with PA0486, an uncharacterized Ser/Thr protein kinase implicated in pyocyanin production and bacterial killing, suggesting a putative in vivo mechanism of action. o Renamed as the Biofilm antibiotic tolerance Regulator (BatR), PA3049 emerges as a key player in P. aeruginosa biofilm maintenance and resistance. These findings provide new insights into bacterial biofilm dynamics and highlight two previously uncharacterized genes with potential implications for combating multidrug-resistant infections
2026-06-26 | PXD062857 | Pride
Project description:This experiment has been annotated by TAIR (http://arabidopsis.org). We examined transcript profiles triggered by three different arabidopsis R genes that recognize distinct Peronospora parasitica isolates. Experimenter name = Thomas Eulgem Experimenter phone = 43 1 4277 54622 Experimenter fax = 43 1 4277 9546 Experimenter department = Institute of Microbiology and Genetics Experimenter address = Institute of Microbiology and Genetics Experimenter address = Dr. Bohrgasse 9 Experimenter address = Vienna Experimenter zip/postal_code = A-1030 Experimenter country = Austria Keywords: strain_or_line_design
2006-08-15 | GSE5532 | GEO
Project description:Semiconductor sequencing of alkaline degraded total RNA from Pyrococcus furiosus annotated for ”The 23S ribosomal RNA from Pyrococcus furiosus is circularly permuted” published in Frontiers in Microbiology”
2020-11-24 | GSE153501 | GEO
Project description:We previously developed a directed evolution model that passaged biofilm populations of in S. Typhimurium under antibiotic stress, which identified trade-offs between biofilm formation and antibiotic resistance, conferred by increased efflux activity (Trampari et al., 2021). We applied this same model to expose biofilm populations of S. Typhimurium to RND efflux inhibitor PAβN or non-antibiotic efflux substrate acriflavine. The aim was to identify mutations responsible for restoring biofilm formation in populations exposed to PAβN and to identify how cells in the biofilm can upregulate efflux in the presence of acriflavine. We hypothesised that comparing the mutations selected in both conditions would identify intersecting regulatory relationships between efflux activity and biofilm formation. Parallel linages of S. Typhimurium were grown on glass beads and in planktonic culture in the presence of a subinhibitory concentration of PAβN or acriflavine (or an untreated control) and passaged into new treated media every 48-72 hours. After one, five and ten passages (corresponding to 2, 17 and 35 days of continuous exposure), the populations were stored, DNA was extracted and sequenced to determine mutations selected in these conditions. We compared mutations from conditions treated with efflux substrates to untreated controls in planktonic and biofilm conditions. To explore phenotypic heterogeneity in these populations, we streaked biofilm populations from passages five and ten onto LB agar and picked three single colonies at random to sequence alongside the populations.
2026-04-15 | E-MTAB-16846 | biostudies-arrayexpress
Project description:Pseudomonas aeruginosa is an opportunistic pathogen that can cause severe infections in immunocompromised individuals, such as patients with cystic fibrosis where it commonly forms biofilms. Ciprofloxacin is used extensively to treat P. aeruginosa infections, but its effectiveness can be significantly reduced due to biofilm formation. Although many individual genes associated with biofilm formation have been characterised, the genetic basis of P. aeruginosa biofilm fitness related to antibiotics challenge remain unexplored. In this study we employed a high-density TraDIS-Xpress library of P. aeruginosa PAO1 to assay the impact of gene disruptions or altered gene expression on biofilm formation at different concentrations of ciprofloxacin. Gene fitness was analysed by comparing the biofilm samples to planktonic samples harvested at 12h, 24h and 48h with and without ciprofloxacin. Gene determinants of survival for biofilms at different stages of maturity in the presence and absence of ciprofloxacin were identified.
2026-06-09 | E-MTAB-16928 | biostudies-arrayexpress