Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

NGS-based miRNome reveals regulation in the miR-34/miR-449 cluster targetome in sinonasal cancers [miRNA-seq]

ABSTRACT: NGS-based miRNome reveals regulation in the miR-34/miR-449 cluster targetome in sinonasal cancers [miRNA-seq]

PROVIDER: PRJNA792156 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR17329500.fastq.gz	Fastqsanger.gz
	SRR17329482.fastq.gz	Fastqsanger.gz
	SRR17329483.fastq.gz	Fastqsanger.gz
	SRR17329484.fastq.gz	Fastqsanger.gz
	SRR17329485.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 19

Similar Datasets

NGS-based miRNome reveals regulation in the miR-34/miR-449 cluster targetome in sinonasal cancers.

Project description:NGS-based miRNome reveals regulation in the miR-34/miR-449 cluster targetome in sinonasal cancers.

| PRJNA792155 | ENA

NGS-based miRNome reveals regulation in the miR-34/miR-449 cluster targetome in sinonasal cancers [miRNA-seq]

Project description:Using NGS-based miRNome, followed by AGO2-RIP-seq, the miR-34c and miR-449a and their direct targets were identified as factors involved in the development and progression of sinonasal cancers (SNCs). Both miR-34c and miR-449a were independent prognostic biomarkers and were associated with patient outcome.

2023-12-28 | GSE192595 | GEO

NGS-based miRNome reveals regulation in the miR-34/miR-449 cluster targetome in sinonasal cancers [RIP-seq]

2023-12-28 | GSE192594 | GEO

NGS-based miRNome reveals regulation in the miR-34/miR-449 cluster targetome in sinonasal cancers [RIP-seq]

Project description:NGS-based miRNome reveals regulation in the miR-34/miR-449 cluster targetome in sinonasal cancers [RIP-seq]

| PRJNA792157 | ENA

AGO2-RIP-Seq reveals miR-34/miR-449 cluster targetome in sinonasal cancers.

Project description:Sinonasal tumours are heterogeneous malignancies, presenting different histological features and clinical behaviour. Many studies emphasize the role of specific miRNA in the development and progression of cancer, and their expression profiles could be used as prognostic biomarkers to predict the survival. Recently, using the next-generation sequencing (NGS)-based miRNome analysis the miR-34/miR-449 cluster was identified as miRNA superfamily involved in the pathogenesis of sinonasal cancers (SNCs). In the present study, we established an Argonaute-2 (AGO2): mRNA immunoprecipitation followed by high-throughput sequencing to analyse the regulatory role of miR-34/miR-449 in SNCs. Using this approach, we identified direct target genes (targetome), which were involved in regulation of RNA-DNA metabolic, transcript and epigenetic processes. In particular, the STK3, C9orf78 and STRN3 genes were the direct targets of both miR-34c and miR-449a, and their regulation are predictive of tumour progression. This study provides the first evidence that miR-34/miR-449 and their targets are deregulated in SNCs and could be proposed as valuable prognostic biomarkers.

| S-EPMC10786392 | biostudies-literature

NGS-based miRNome reveals regulation in the miR-34/miR-449 cluster targetome in sinonasal cancers.

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-12-28 | GSE192596 | GEO

Transcription profiling by array of mouse pachytene cells from mir-34bc+/-;449+/- and mir-34bc-/-;449-/- male animals.

Project description:Analysis of gene expression (mRNA profiles) from mouse pachytene cells. Mir-34bc+/-;449+/- samples are compared against mir-34bc-/-;449-/- samples .

2014-08-17 | E-MTAB-2668 | biostudies-arrayexpress

Transient transfection of miR-449 family in the hepatocellular carcinoma cell line HLE

2015-11-06 | GSE74710 | GEO

Gene expression changes in the respiratory epithelium of miR-34/449 knockout mice

Project description:MicroRNA ablation prevents multiciliogenesis via cell cycle deregulation MicroRNAs (miRNAs) have been implicated in various biological processes but have been most frequently described to inhibit proliferation and tumorigenesis. Here we describe an essential function of the miR-34/449 family during differentiation of multiciliated cells that is mediated by their well-described suppression of cell cycle progression. Constitutive deletion of all six members of this miRNA family triggers a derepression of multiple cell cycle-promoting proteins, thereby preventing epithelial cells from exiting the cell cycle and to maintain in a quiescent state. As a result, formation of motile multicilia is strongly inhibited in several tissues including the respiratory epithelium and the fallopian tube. Consequently, mice lacking miR-34/449 genes display infertility as well as severe chronic airway disease leading to postnatal death. These results show how miRNA-mediated repression of the cell cycle is required to allow multiciliogenesis to proceed during epithelial differentiation.

2017-09-12 | GSE92296 | GEO

Transient transfection of miR-449 family in the hepatocellular carcinoma cell line HLE

Project description:In this study, we comparatively analyzed the members of the miR-449 family (miR-449a, miR-449b, and miR-449c) with regards to their target genes and functional effects in hepatocellular carcinoma (HCC). Microarray analysis after transient transfection of miR-449a, miR-449b, and/or miR-449c in the HCC cell line HLE identified putative target genes of miR-449a, miR-449b, and miR-449c. For transient overexpression of miRNAs, the HCC cell line HLE was transfected with 50 nM Allstars Negative Control or miScript miRNA mimics. MiRNA mimics for miR-449a, miR-449b, and miR-449c were either single transfected or cotransfected in equimolar amounts (16.7 nM each). Global mRNA expression profiling was performed utilizing pooled RNA of three biological replicates per microarray and two microarrays per condition.

2015-11-06 | E-GEOD-74710 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data