Project description:We used Solexa sequencing technology to probe the gene expression of dorsal skin tissues from three full-sib Rex rabbits of different colors. The number of expressed genes in each sample was approximately 14,700. Compared with the chinchilla Rex rabbit control sample, the numbers of genes that exhibited greater than a 2-fold change in expression with a false discovery rate of ≤0.001 in the black rabbit sample and white rabbit sample were 1,809 and 460, respectively, and the number of common differentially expressed genes was 257.Of the 257 genes, 32 genes that were upregulated in sample B and downregulated in sample W. The FABP7 gene was the only gene that was downregulated in the B sample and upregulated in the W sample
Project description:Inspection of Hi-C and ChIP-seq data suggests that condensin DC is an X-chromosome enriched loop extruder that stalls at rex sites. Insertion of rex site on X-chromosome suggests orientation independent barrier function of rex sites. Insertion of rex site on chromosome-II results in recruitment and spreading of condensin DC that coincides with TAD formation.
2022-06-14 | GSE206065 | GEO
Project description:Screening and Analysis of Response Genes of Rex Rabbit SLC7A11 in Melanocytes
Project description:ChIP-seq for the SDC-3 subunit of the dosage compensation complex in wild-type N2 C. elegans and Hi-C in C' elegans strains with deleted rex and extra rex sites
Project description:In embryos, DCC binding across the length of the ~17.7 Mb X chromosome initiates at a set of recruitment elements on the X (rex). Individual rex sites collectively contribute to recruitment and repression, thus deletion of one or few rex sites causes subtle changes, as measured by mRNA-seq in mixed-stage embryos. We previously constructed a strain deleting rex-1, located ~6 kb downstream of dpy-23 (https://doi.org/10.7554/eLife.23645). Here we performed mRNA-seq in rex-1 deletion embryos.
Project description:This study was undertaken in order to characterize the functions of Rex-1 and identify potential Rex-1 target genes.Both alleles of the Rex-1 gene were disrupted in J1 mouse embryonic stem cells. Gene expression levels in one of the resulting Rex-1 knockout cell lines was compared to that of J1 wild type cells. Keywords: cell type comparison
Project description:Localis-REX has been developed by our lab.In the current project, we aim to study transcriptomics changes under Localis-REX within different subcelluar locations. Localis-REX coupled RNA-seq would give us a borad idea of mRNA changing under our reaction conditon over mutiple controls
