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Single cell ATAC-seq

ABSTRACT: single cell ATAC-seq

PROVIDER: PRJNA794166 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17431352_1.fastq.gz	Fastqsanger.gz
	SRR17431352_2.fastq.gz	Fastqsanger.gz
	SRR17431353_1.fastq.gz	Fastqsanger.gz
	SRR17431353_2.fastq.gz	Fastqsanger.gz
	SRR17431354_1.fastq.gz	Fastqsanger.gz

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Project description:Single cell ATAC-seq (scATAC-seq) was performed on macaque embryonic stem cell-derived cerebral organoids. scATAC-seq was performed on day 60 (2 months old cerebral organoid).

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Single-cell ATAC-seq of bonobo cerebral organoids

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Project description:Single cell ATAC-seq (scATAC-seq) was performed at various stages of differentiation of human pluripotent stem cells to 4 month old cerebral organoids. scATAC-seq was performed on the following days of differentiation: day 0 (pluripotent stem cell), day 4 (embryoid body), day 10 (neuroectoderm), day 15 (neuroepithelium), day 30 (1 month old cerebral organoid), day 60 (2 months old cerebral organoid), and day 120 (4 months old cerebral organoid).

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Single-cell ATAC-seq of chimpanzee cerebral organoids

Project description:Single cell ATAC-seq (scATAC-seq) was performed at various stages of differentiation of chimpanzee induced pluripotent stem cells (iPSC) to 4 month old cerebral organoids. scATAC-seq was performed on the following days of differentiation: day 0 (pluripotent stem cell), day 4 (embryoid body), day 10 (neuroectoderm), day 15 (neuroepithelium), day 30 (1 month old cerebral organoid), day 60 (2 months old cerebral organoid), and day 120 (4 months old cerebral organoid).

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A simple and robust plate-based single-cell ATAC-seq on various tissues and cell lines

Project description:The assay for transposase-accessible chromatin using sequencing (ATAC-seq) is widely used to identify regulatory regions throughout the genome. However, only a few studies have been done at the single cell level (scATAC-seq) due to technical difficulties. Here we developed a simple and robust plate-based scATAC-seq method, combining upfront bulk tagmentation with single-nuclei sorting, to investigate open chromatin regions. We applied this method on mouse splenocytes and unbiasedly revealed key regulatory regions and transcription factors that define each cell (sub)type.

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2018-10-01 | E-MTAB-6116 | biostudies-arrayexpress

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