Ontology highlight
ABSTRACT:
PROVIDER: PRJNA795832 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR17532854.fastq.gz | Fastqsanger.gz | |||
| SRR17532855.fastq.gz | Fastqsanger.gz | |||
| SRR17532856.fastq.gz | Fastqsanger.gz | |||
| SRR17532857.fastq.gz | Fastqsanger.gz | |||
| SRR17532858.fastq.gz | Fastqsanger.gz |
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Similar Datasets
Project description:Experiment to understand relationships between sheep rumen wall transcriptome and microbial methane emissions
2017-12-30 | GSE81847 | GEO
Project description:<p>Gut microbiota plays a significant role in maintaining the homeostasis of the gut internal environment, and the volatile fatty acids (VFAs) produced by it are the main source of energy utilization for the host. The heart, as a key metabolic organ of the body, its energy metabolism efficiency directly affects the body's tolerance to the hypoxic environment at high altitudes. To reveal the dynamic regulatory relationship between the rumen and the heart of Hu sheep during their response to the high-cold and hypoxic environment, this study conducted transcriptome sequencing on the hearts of Hu sheep and Tibetan sheep, and carried out interaction analysis of the differentially expressed genes with rumen microbiota, VFAs, and metabolites. The results showed that: A total of 616 differentially expressed genes (P＜0.05) were identified in the hearts of sheep of different breeds, among which 437 genes were up-regulated and 179 genes were down-regulated. By comparing with known transcription factors, it was found that genes highly expressed in Hu sheep, such as ATP2A3, NPPB, PDE3A, SLC25A4, and AKT3, were significantly enriched in the cGMP-PKG signaling pathway. In the study of the interaction between rumen microbial genera and heart-related differentially expressed genes, it was found that microbial genera such as Candidatus Saccharimonas and Succiniclasticum had a close positive correlation (P＜0.05) with genes related to cardiac energy metabolism; The interaction analysis between the differentially expressed genes and VFAs showed that acetic acid, propionic acid, butyric acid, and valeric acid participated in the regulation of gene expression in a positive and synergistic manner. WGCNA showed that different metabolite modules were concentrated and enriched in Metabolic pathways and participated in the process of cardiac energy metabolism. In addition, metabolites such as Arachidonate, Adenine, and 6-Keto-prostaglandin F1alpha had a positive regulatory relationship with SLC25A4 and AKT3 and were involved in cardiac energy metabolism. This study revealed that Hu sheep can conduct directional regulation of cardiac metabolism through rumen microbiota and metabolites to cope with the hypoxic stress at high altitudes, providing an important reference for a deeper understanding of the response mechanism of Hu sheep to the hypoxic environment at high altitudes.</p>
2025-09-11 | MTBLS12982 | MetaboLights
Project description:<p>Introduction: This study aimed to investigate the effects of natural forage from different regions (saline-alkali and non-saline-alkali areas) on the rumen microbiota, muscle metabolites, and meat quality of Tibetan sheep. </p><p>Methods: Targeted and non-targeted metabolomics were used to comprehensively analyze both pasture and meat quality, metabolites. Additionally, 16S rDNA sequencing was employed to analyze the rumen microbial community structure of Tibetan sheep.</p><p>Results: The results showed that the natural saline-alkali forage (HG group) had higher protein content, lower fiber content, higher relative feed value, and better quality. Metabolomic analysis revealed significant accumulation of flavonoids and upregulation of amino acid metabolism in the HG group. Additionally, the natural saline-alkali forage significantly increased amino acid deposition in Tibetan sheep muscle, markedly enhanced the redness value (a*), and significantly reduced the yellowness value (b*). Furthermore, the natural saline-alkali forage altered the rumen fermentation patterns in Tibetan sheep, leading to a significant increase in the abundance of F082 and WCHB1-41, while significantly reducing the abundance of Prevotellaceae_UCG-003. Correlation analysis revealed that these microbial taxa were significantly influenced by the natural saline-alkali forage,while also showing significant associations with muscle quality parameters (a*, b*) and metabolites (cysteine, C18:1n9, etc.).</p><p>Discussion: Overall, the natural saline-alkali forage demonstrated superior quality and metabolite content compared to natural non-saline-alkali forage. Furthermore, this saline-alkali forage significantly influenced the abundance of specific rumen microbiota in Tibetan sheep, consequently regulating</p>
2025-07-19 | MTBLS12748 | MetaboLights
Project description:<p> Introduction: This study aimed to investigate the effects of natural forage from different regions (saline-alkali and non-saline-alkali areas) on the rumen microbiota, muscle metabolites, and meat quality of Tibetan sheep. </p><p> Methods: Targeted and non-targeted metabolomics were used to comprehensively analyze both pasture and meat quality, metabolites. Additionally, 16S rDNA sequencing was employed to analyze the rumen microbial community structure of Tibetan sheep.</p><p> Results: The results showed that the natural saline-alkali forage (HG group) had higher protein content, lower fiber content, higher relative feed value, and better quality. Metabolomic analysis revealed significant accumulation of flavonoids and upregulation of amino acid metabolism in the HG group. Additionally, the natural saline-alkali forage significantly increased amino acid deposition in Tibetan sheep muscle, markedly enhanced the redness value (a*), and significantly reduced the yellowness value (b*). Furthermore, the natural saline-alkali forage altered the rumen fermentation patterns in Tibetan sheep, leading to a significant increase in the abundance of F082 and WCHB1-41, while significantly reducing the abundance of Prevotellaceae_UCG-003. Correlation analysis revealed that these microbial taxa were significantly influenced by the natural saline-alkali forage,while also showing significant associations with muscle quality parameters (a*, b*) and metabolites (cysteine, C18:1n9, etc.).</p><p>Discussion: Overall, the natural saline-alkali forage demonstrated superior quality and metabolite content compared to natural non-saline-alkali forage. Furthermore, this saline-alkali forage significantly influenced the abundance of specific rumen microbiota in Tibetan sheep, consequently regulating</p>
2025-07-19 | MTBLS12747 | MetaboLights
Project description:<p>Background: Hu sheep, renowned for their prolificacy and economic value, have been introduced to Xinjiang for intensive farming operations. However, their suboptimal adaptation to local harsh environments limits growth efficiency and health management. In contrast, Duolang sheep, an indigenous Xinjiang breed, exhibit exceptional environmental resilience, largely attributed to their unique rumen microbiome enriched with fiber-degrading bacteria, which enhance roughage utilization and stress tolerance. To address Hu sheep’s adaptive challenges, this study applies Ligilactobacillus salivarius KS1018, a probiotic strain isolated from Duolang sheep, to Hu sheep, investigating its role in improving rumen microbial homeostasis, metabolic adaptation, and physiological resilience under Xinjiang’s environmental conditions.</p><p>Results: Thirty-two male Hu lambs were allocated into four groups: Control (C) and three treatment groups receiving daily doses of 0.5×109 CFU/d (low-dose group, LLS, 1.0×109 CFU/d (medium-dose group, MLS), or 1.5×109 CFU/d (high-dose group, HLS) for 56 days. Although growth performance did not reveal significant enhancement, supplementation with the high dose of L. salivarius KS1018 (HLS group) significantly reduced ruminal NH3-N (p < 0.05) and increased total VFAs (p = 0.043), alongside elevated serum triglycerides (p = 0.021) and β-hydroxybutyrate (p < 0.05), indicating enhanced nitrogen utilization and ketogenesis. Rumen metabolomics revealed dose-dependent metabolic shifts: group HLS prioritized oxidative phosphorylation and pyrimidine metabolism (p < 0.001), while group MLS upregulated branched-chain amino acid biosynthesis (p < 0.01). Procrustes analysis confirmed strong microbiome-metabolome coordination (M2 = 0.56, p = 0.001), linking Lachnospira and Negativicutes enrichment to VFA production and redox homeostasis. Group HLS exhibited a decline in ruminal pH, suggesting a potential risk of subacute acidosis at higher doses.</p><p>Conclusions: L. salivarius KS1018 optimizes rumen metabolism through microbial-host crosstalk, improving nitrogen efficiency and stress resilience in Hu sheep. The strain promoted ketogenesis and oxidative phosphorylation pathways, which alleviated oxidative stress and enhanced immune function under environmental challenges. Notably, the arid-adaptive genetic traits likely contribute to stabilizing rumen microbial networks, mimicking the superior roughage utilization capacity of its native Duolang sheep host. Our findings highlight the potential of L. salivarius KS1018 as a metabolic modulator in ruminant nutrition, redefining its role beyond traditional growth-centric applications.</p>
2025-08-02 | MTBLS12659 | MetaboLights
Project description:In order to investigate the diurnal oscillations of ruminal bacteria, and their responses to the changes in different feeding patterns, we conducted an animal experiment by feeding the sheep ad libitum with a hay-based diet (50% of alfalfa hay and 46% of oats hay) and a grain-based diet (45% of corn meal and 11% of soybean meal) for 30 days, and ruminal fluid samples were collected at six different timepoints from T2 to T22 in one day, and the composition and diversity of the bacterial communities in rumen microbiomes of the sheep in the Grain-diet and Hay-diet groups at different timepoints were analyzed through 16S rRNA sequencing.
2025-01-08 | GSE286149 | GEO