Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Distinct Mannheimia haemolytica serotypes isolated from fatal infections in veal calves and dairy cows

PROVIDER: PRJNA796182 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR17604100_1.fastq.gz	Fastqsanger.gz
	SRR17604100_2.fastq.gz	Fastqsanger.gz
	SRR17604101_1.fastq.gz	Fastqsanger.gz
	SRR17604101_2.fastq.gz	Fastqsanger.gz
	SRR17604102_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 192

Similar Datasets

Proteomic Analysis of Outer Membrane Vesicles Derived from the Type A5 Strain of Mannheimia haemolytica

Project description:Mannheimia haemolytica (M. haemolytica) cause mastitis in sheep, acute sepsis in newborn lambs, and co-infections with various pathogens, leading to bovine respiratory disease syndrome (BRDS), these infections have resulted in significant economic losses to both domestic and international farming industries. An in-depth understanding of the pathogenic mechanisms of M. haemolytica is crucial for the prevention and control of this disease. Outer membrane vesicles (OMVs) play a vital role in bacterial pathogenesis, serving as key mediators of interactions between Gram-negative bacteria and their hosts. However, the specific role of OMVs in the pathogenic process of M. haemolytica remains poorly understood. To address this, we isolated OMVs from the Mannheimia haemolytica Type A5 strain (MH-5) using ultracentrifugation and subsequently characterized their secretory properties, protein composition, and immunogenicity through electron microscopy, liquid chromatography-tandem mass spectrometry (LC-MS/MS), and cellular experiments. The electron microscopy results indicated that the MH-5 strain secreted OMVs under natural growth conditions. Proteomic and bioinformatics analyses revealed that these OMVs contained 282 proteins, with significant enrichment in proteins related to immunity, iron metabolism, and catalytic activity. Cellular experiments demonstrated that, compared to the control group, the OMVs group exhibited a significant increase in the mRNA expression of IL-1β, IL-6, and TNF-α, with secretion levels increasing in a dose-dependent manner, thereby enhancing the inflammatory response. These findings lay the groundwork for further exploration of the role of OMVs in the pathogenesis of M. haemolytica and provide insights for the development of effective vaccines and antibiotics against this pathogen.

2025-06-30 | PXD064633 | Pride

Mannheimia haemolytica USMARC_2286

Project description:Mannheimia haemolytica from non-diseased calves

| PRJNA212438 | ENA

Multi-omics analyses in cows and their female progeny in the context of direct and time-lagged heat stress effects

Project description:A heat stress (HS) cattle research design was implemented to study HS effects on the three different “omics features” methylations, gene expressions and metabolic pattern from a direct perspective in pregnant cows and from an indirect time-lagged intergenerational perspective in offspring (the respective F1 and as F1 offspring before calving). In this regard, German Holstein dairy cows and their calves were blood sampled for DNA and RNA extraction and for metabolic phenotyping, and allocated to HS and respective control groups (the cows (dams) as well as their calves) according to a temperature-humidity threshold of 60.

2025-05-29 | GSE289946 | GEO

gut metagenome

Project description:Bovine fecal metagenome from dairy veal calves

| PRJNA645414 | ENA

Escherichia coli

Project description:Escherichia coli genomes from dairy and veal calves

| PRJNA402010 | ENA

Blood immune transcriptome analysis of artificially fed dairy calves and naturally suckled beef calves from birth to 7 days of age

Project description:The aim of the overall study was to investigate the development of immune competence in artificially reared dairy calves and in two breeds of naturally suckled beef calves over the first 168h of life. Dairy calves were fed 5% total body weight of colostrum, with beef calves monitored to ensure natural ingestion of colostrum. Blood samples were taken from all calves at 24h 48h 72h and 168h, and analysed for alterations to immunes genes.

2018-10-29 | GSE114432 | GEO

Comparative genomic hybridization of Staphylococcus aureus recovered from a variety of sources including mastitis in dairy species

Project description:Staphylococcus aureus is recognized worldwide as a major pathogen causing clinical or subclinical intramammary infections in all the dairy species (sheep, goats and cows). The present study was designed to comparatively investigate 65 S. aureus isolates recovered from dairy sheep and S. aureus suclinical mastitis from cows (n=21) and goats (n=22), for the presence of 190 putative virulence determinants with a single-dye DNA microarray and PCR. The probes (65 mer) were mainly designed from the S. aureus Mu50. The extracted DNA of each strain was labelled with Cy5. The microarray results were validated with PCR.The genomic comparative study with the DNA microarrays showed lineage and species specificity genes leading to the host-specific pathogenic traits of S. aureus in dairy species.

2008-07-01 | E-TABM-288 | biostudies-arrayexpress

Potassium balance in lactating and non-lactating dairy cows

Project description:M. Berg, J. Plöntzke, S. Leonhard-Marek, K.E. Müller & S. Röblitz. A dynamic model to simulate potassium balance in dairy cows. Journal of Dairy Science 100, 12 (2017). High-performing dairy cows require a particular composition of nutritional ingredients, adapted to their individual requirements and depending on their production status. The optimal dimensioning of minerals in the diet, one being potassium, is indispensable for the prevention of imbalances. Potassium balance in cows is the result of potassium intake, distribution in the organism, and excretion, and it is closely related to glucose and electrolyte metabolism. In this paper, we present a dynamical model for potassium balance in lactating and nonlactating dairy cows based on ordinary differential equations. Parameter values were obtained from clinical trial data and from the literature. To verify the consistency of the model, we present simulation outcomes for 3 different scenarios: potassium balance in (1) nonlactating cows with varying feed intake, (2) nonlactating cows with varying potassium fraction in the diet, and (3) lactating cows with varying milk production levels. The results give insights into the short- and long-term potassium metabolism, providing an important step toward the understanding of the potassium network, the design of prophylactic feed additives, and possible treatment strategies.

2024-09-02 | BIOMD0000000849 | BioModels

Mannheimia haemolytica strain:16041065 BH

Project description:Mannheimia haemolytica genome sequencing

| PRJNA349069 | ENA

Mannheimia haemolytica

Project description:Mannheimia haemolytica Genome sequencing

| PRJNA774133 | ENA