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CUT&Tag for GATA factors, H3K27ac and H3K27me3 in female XEN and TS cells

ABSTRACT: CUT&Tag for GATA factors, H3K27ac and H3K27me3 in female XEN and TS cells

PROVIDER: PRJNA798743 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17665470_1.fastq.gz	Fastqsanger.gz
	SRR17665470_2.fastq.gz	Fastqsanger.gz
	SRR17665471_1.fastq.gz	Fastqsanger.gz
	SRR17665471_2.fastq.gz	Fastqsanger.gz
	SRR17665472_1.fastq.gz	Fastqsanger.gz

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CUT&Tag for GATA factors, H3K27ac and H3K27me3 in female XEN and TS cells

Project description:As the master regulator of X-chromosome inactivation (XCI), the Xist RNA is expressed nearly ubiquitously in female mice. Xist is only absent in the germ line and at the pluripotent state. Xist is generally assumed to be expressed “by default” in females, while being actively repressed in the few tissues where it is silent. Whether activating mechanisms also contribute remained largely unknown. Through a pooled CRISPR screen we identify the GATA family of transcription factors as potent direct activators of Xist. We describe a GATA-responsive regulatory element (RE79), located ~100 kb upstream of the Xist promoter. In cell lines derived from the two extraembryonic lineages, XEN and TS cells, where imprinted Xist expression is maintained, RE79 is bound by different sets of GATA factors expressed in those tissues. Here we use CUT&Tag on GATA factors to profile their involvement at the X inactivation center in XEN and TS cells.

2022-01-26 | GSE194014 | GEO

H3K27me3 and H3K4me2 profiles along the length of the X chromosome in trophoblast stem (TS) cells and Extra-embryonic endoderm stem cells (XEN), ChIP-chip

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H3K27me3 and H3K4me2 profiles along the length of the X chromosome in trophoblast stem (TS) cells and Extra-embryonic endoderm stem cells (XEN), ChIP-chip

Project description:This analysis includes H3K27me3 and H3K4me2 profiles along the length of the X-chromosome in male (F2) and female (F3) TS cells and in male (GHP7/7) and female (GHP7/9) XEN cells. Analysis of H3K27me3 and H3K4me2 profiles in four different cell populations. Two independent biological replicates for each cell population are provided. The array includes approx. 162,5 Mb of the X chromosome and approx. 14,6 Mb of chromosome 17 as a control.

2014-06-01 | E-GEOD-50587 | biostudies-arrayexpress

H3K27me3 profiles along the length of the X chromosome in trophoblast stem (TS) cells, ChIP-chip

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H3K27me3 profiles along the length of the X chromosome in trophoblast stem (TS) cells, ChIP-chip

Project description:This analysis includes H3K27me3 profiles along the length of the X-chromosome in male (F2) and female (K4) TS cells and in female TS cells showing local reversals of imprinted X-chromosome inactivation (K4GFP). Data also includes H3K27me3 ChIP-chip profiles in Eed-/- mutant male and female TS cells obtained from Magnuson laboratory (Kalantry S. et al., Nat Cell Biol, 2006). Analysis of H3K27me3 profiles in five different cell populations. Three independent biological replicates for each wild-type female cell population and two independent biological replicates for the wild-type male cell population are provided. The ChIP-chip analysis was also carried out in Eed-/- male and female TS cells lacking H3K27me3 for comparison (one replicate). The array includes approx. 162,5 Mb of the X chromosome and approx. 14,6 Mb of chromosome 17 as a control.

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H3K27me3 profiles along the length of the X chromosome in trophoblast stem (TS) cells showing inverted X-inactivation profiles, ChIP-chip

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Distribution of H3K27me3, H3K27ac, and H3K4me1 in E(z) temperature sensitive cells

Project description:PcG protein complex PRC2 is a methyltransferase specific for histone H3 lysine27, and H3K27me3 is essential for stable transcription silencing. Here we have determined genomic distributions of H3K27me3, H3K27ac, and H3K4me1 in cultured Drosophila E(z) temperature sensitive cells at 25°C and 31°C where E(z) is largely inactivated by hybridization of ChIP products with tiling microarrays. Genomic distributions of H3K27me3, H3K27ac, and H3K4me1 in cultured Drosophila E(z) temperature sensitive cells (EZ2-2) at 25°C and 31°C where E(z) is largely inactivated by hybridization of ChIP products with tiling microarrays

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2016-07-03 | E-GEOD-68536 | biostudies-arrayexpress

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Project description:Transcriptional regulation relies upon multiple nuclear processes among which histone modifications constitute a key component. To further decipher the nuclear changes resulting from TTC7A deficiency, we performed ChIP-Seq of H3K27ac and H3K27me3 histone marks associated with transcriptional activation and repression respectively.

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TTC7A deficiency Impacts the H3K27ac and H3K27me3 histone marks

Project description:TTC7A deficiency Impacts the H3K27ac and H3K27me3 histone marks

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