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Trichoderma atroviride

ABSTRACT: Identification of mycoparasitism-related genes in Trichoderma atroviride: A holistic comparative study on self confrontation and host interaction by de novo transcriptome sequencing

PROVIDER: PRJNA79919 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR094192.fastq.gz	Fastqsanger.gz
	SRR094397.fastq.gz	Fastqsanger.gz
	SRR094398.fastq.gz	Fastqsanger.gz
	SRR094399.fastq.gz	Fastqsanger.gz

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Unveiling Oak Endophyte Dynamics: Cadophora luteo-olivacea and Trichoderma atroviride from Pathogenicity Tests to Omics Insights

Project description:Pedunculate oak (Quercus robur) is a foundation tree species in European forests and reforestation programs, and forest nurseries represent key phytosanitary bottlenecks where asymptomatic planting stock can disseminate cryptic pathogens. Cadophora luteo-olivacea is frequently associated with grapevine trunk diseases and has been recovered from diverse woody hosts, yet its pathogenic potential on oak and its interactions with antagonistic fungi remain unresolved. Here, we combine pathogenicity testing, dual-culture confrontation assays and time-resolved, spatially resolved contact-zone proteomics and metabolomics to connect detection to causality and mechanism in an oak associated Cadophora–Trichoderma system. We fulfill Koch’s postulates for C. luteo-olivacea on Q. robur seedlings and quantify inhibition of Cadophora by a natural isolate of Trichoderma atroviride. Contact-zone proteomics at 4 and 8 days post-contact reveals a staged, interface-localized antagonistic program dominated by fungal cell-wall targeting hydrolases (including chitinases and β-1,3-glucanases), secreted proteases, oxidoreductases, transporters and small secreted proteins, alongside factors consistent with adhesion and self-protection. Together, these complementary assays provide the first experimental evidence that C. luteo-olivacea is pathogenic on Q. robur and delineate mechanistic signatures of T. atroviride mycoparasitism at the interaction front. This framework links nursery-relevant pathogen screening to mechanistic readouts and informs evaluation of biocontrol potential in a foundation forest species.

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RNAi components regulate the microparasitism in Trichoderma atroviride

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Project description:This SuperSeries is composed of the following subset Series: GSE19832: Trichoderma virens transcript levels during mycoparasitism GSE23382: Trichoderma atroviride transcript levels during mycoparasitism GSE23410: Trichoderma reesei transcript levels during mycoparasitism Refer to individual Series

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RNAi components regulate the micoparasitism in Trichoderma atroviride

Project description:Trichoderma atroviride is a fungus capable of establishing symbiotic relationships with plants, however, its main lifestyle is a saprophyte. Due to these characteristics, it must face a great quantity of microorganisms, and be able to compete for nutrients. T. atroviride is considered a necrotrophic mycoparasite and has developed the ability to kill other organisms and obtain nutrients from them. The object of this work is to explore the role of small RNAs in mycoparasitism. To this end, we obtained RNA-Seq libraries from the interactions of T. atroviride against Alternaria alternata and Rhizoctonia solani. The transcriptomes were obtained from three stages during mycoparasitsm: before contact (BC), contact (C), and after contact (AC).

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Trichoderma atroviride

Project description:Trichoderma atroviride transcript levels during mycoparasitism

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Gene expression response of the mycoparasite Trichoderma atroviride to confrontation with the host Rhizoctonia solani in the wild-type and tmk1 gene deletion mutants

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Expression array for Trichoderma atroviride in mycoparasitic interaction with Rhizoctonia solani

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