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Transcriptomics analysis reveals the molecular mechanisms of the antibacterial activity of gallium nitrate against Acinetobacter baumannii isolated from bloodstream infections

ABSTRACT: Transcriptomics analysis reveals the molecular mechanisms of the antibacterial activity of gallium nitrate against Acinetobacter baumannii isolated from bloodstream infections

PROVIDER: PRJNA800015 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17717469_1.fastq.gz	Fastqsanger.gz
	SRR17717469_2.fastq.gz	Fastqsanger.gz
	SRR17717470_1.fastq.gz	Fastqsanger.gz
	SRR17717470_2.fastq.gz	Fastqsanger.gz
	SRR17717471_1.fastq.gz	Fastqsanger.gz

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Transcriptomics analysis reveals the molecular mechanisms of the antibacterial activity of gallium nitrate against Acinetobacter baumannii isolated from bloodstream infections

Project description:Purpose: The aim of this study was to explore the antibacterial effect and molecular mechanism of gallium nitrate [Ga(NO 3 ) 3 ] against Acinetobacter baumannii from bloodstream infection. Methods: A total of 40 A. baumannii with different antimicrobials susceptibility patterns were isolated from bloodstream infections. In vitro antibacterial effect of Ga(NO 3 ) 3 was analyzed by micro-dilution method and time-kill assay. The effect of ferric chloride/heme on the antibacterial effect of Ga(NO 3 ) 3 was evaluated. Transcriptome sequencing was performed to elucidate the antibacterial mechanism of gallium nitrate. Mouse infection model was conducted to explore the in vivo efficacy of gallium nitrate . Results: Ga(NO 3 ) 3 exhibited excellent antibacterial effect in RPMI 1 640 medium containing 10% human serum (MICs ranged from 0.06 μg/mL to 0.125 μg/mL), whereas its antibacterial effect was weakened by exogenous ferric chloride/heme. Ga(NO 3 ) 3 inhibited A. baumannii growth in a dose- and time- dependent manner. Ga(NO 3 ) 3 exerted antibacterial effect by up-regulating the expression of genes associated with biosynthesis and transport of siderophore and disrupting multiple iron dependent metabolism processes. Ga(NO 3 ) 3 significantly reduced bacterial load in the neutropenic mouse thigh infection model. Conclusions: This study revealed that Ga(NO 3 ) 3 had excellent antibacterial activity both in vivo and in vitro . It might be a potential drug for treating bloodstream infections of A. baumannii

2022-01-27 | GSE194255 | GEO

Insights into the Synergistic Antibacterial Activity of Silver Nitrate with Potassium Tellurite Against Pseudomonas aeruginosa

Project description:To investigate the individual and synergistic antibacterial mechanisms of silver nitrate and potassium tellurite, Pseudomonas aeruginosa cultures were exposed to a shock treatment of these metal salts (individually and in combination) at inhibitory concentrations. Differential gene expression was identified by contrasting each metal salt challenge against a PBS-treated control.

2023-06-15 | GSE232832 | GEO

CC398 bloodstream infections in DK

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Acclimation of the Escherichia coli transcriptome after 10 hours of incubation in the presence of gallium nitrate at sublethal levels.

Project description:Gallium nitrate has been explored as an FDA-approved alternative to treat antibiotic-resistant bacterial strains. Questions remain as it relates to the full-systems response elicited to counteract its toxic effects. We explored the transcriptomic response profile of Escherichia coli K12 BW25113 when challenged to grow planktonically for 10 hours in the presence of a sublethal concentration of gallium nitrate (1.25 mM, 319.6 µg/mL). Our results yielded significant changes in gene expresson levels: 581 genes were up-regulated, while 791 were down-regulated in our gallium nitrate treatment. Several biological processes became dysregulated to cope with the presence of this metal stressor, including iron homeostasis, sulfate metabolism, oxidative and nitrosative stress response, cysteine biosynthesis, anaerobic respiration, toxin-antitoxin interactions, and DNA repair. Altogether, this work provides a valuable snapshot of how our indicator strain adapts to this metal-induced stress. This is a significant step in understanding how bacteria can adjust their physiology to coexist with sublethal concentrations of metal-based antimicrobials.

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Project description:We examine the role of the gastrointestinal lymphatic tissue (GALT) in the formation of bloodstream infections in leukemia

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Insights into the Synergistic Antibacterial Activity of Silver Nitrate with Potassium Tellurite Against Pseudomonas aeruginosa

Project description:Insights into the Synergistic Antibacterial Activity of Silver Nitrate with Potassium Tellurite Against Pseudomonas aeruginosa

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nitrate induction - nlp mutants-Nitrate induction - nlp mutants

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