Ontology highlight
ABSTRACT:
PROVIDER: PRJNA801052 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Bud dormancy release in woody plants is crucial for survival, regrowth, flowering and fruiting. Tree peony (Paeonia suffruticosa), an importantly ornamental and economic plant undergoes bud endodormancy in winter, and effective chilling duration and exogenous gibberellins (GA) can effectively break the dormancy, however, its epigenetic regulation mechanism remains poorly understood. Here, western blotting revealed that H3K4me3 but not H3K4me1 and H3K4me2 was associated with chilling- and gibberellin (GA3)- induced dormancy release. Chromatin immunoprecipitation sequencing (ChIP-seq) combined with RNAseq results revealed that H3K4me3 enriched near transcription start sites (TSS), and was correlated with the transcriptional activation of dormancy release-related genes (PsEBB3, PsCYCD3.1, PsCYCD3.3, PsBG6, and so on). H3K4me3 enrichment genes (HEGs) common to the differentially-expressed genes (DEGs) were mainly enriched in KEGG pathways such as plant hormone signaling transduction and MAPK signaling. Four COMPASS-like component homologs were screened, among them, PsWDR5a, PsRBL, and PsASH2R were up-regulated by the prolonged chillings and GA3 treatment. PsRBL interacted with PsWDR5a and PsASH2R using yeast two-hybrid, luciferase complementation. Yeast three- hybrid and co-immunoprecipitation assays confirmed that PsRBL bridged the other proteins to form a nuclear-localized complex. Functional analyses showed that PsWDR5a, PsRBL, and PsASH2R significantly promoted budburst and elevated genomic H3K4me3 levels. Our fingings would provide novel insights into the epigenetic regulation of dormancy transitions in woody perennials.
2026-08-04 | GSE304600 | GEO
Project description:Tree peony (Paeonia ostii section Moutan DC.) is known for its excellent ornamental and medicinal values. In 2011, seeds from P. ostii have been identified as novel resource of alpha-linolenic acid (ALA) for seed oil production and development in China. However, the molecular mechanism on biosynthesis of unsaturated fatty acids in tree peony seeds remains unknown. Therefore, transcriptome data is needed to better understand the underlying mechanisms. In this study, lipids accumulation contents were measured using GC-MS methods across developing tree peony seeds, which exhibited an extraordinary ALA content (49.3%) in P. ostii mature seeds. Transcriptome analysis was performed using Illumina sequencing platform. A total of 144 million 100-bp paired-end reads were generated from six libraries, which identified 175,874 contigs. In the KEGG Orthology enrichment of differentially expressed genes, lipid metabolism pathways were highly represented categories. Using this data we identified 388 unigenes that may be involved in de novo fatty acid and triacylglycerol biosynthesis. In particular, three unigenes (SAD, FAD2 and FAD8) encoding fatty acid desaturase with high expression levels in the fast oil accumulation stage compared with the initial stage of seed development were identified.
2017-03-04 | E-MTAB-3377 | biostudies-arrayexpress
Project description:Paeonia suffruticosa ‘Luoyang Hong’Transcriptome
| PRJNA359126 | ENA
Project description:Paeonia ostii x Paeonia suffruticosa Raw sequence reads
| PRJNA278925 | ENA