Ontology highlight
ABSTRACT:
PROVIDER: PRJNA801314 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Experimental design: 1 genotype: PI- (resistant USDA Plant Introduction (PI462312) line containing SBR Rpp3 resistance gene) 3 treatments: Virulent soybean rust (Phakopsora pachyrhizi Tw80-2) challenge, avirulent soybean rust challenge (Hw94-1) & mock infection 3 replications 6 time points: 12, 24, 72, 144, 216 and 288 hours after inoculation TOTAL: 54 Affymetrix GeneChip(R) Soybean Genome Arrays Mock treatment: 0.01% Tween 20 Hawaii 94 treatment: 500,000 spores per ml in 0.01% Tween 20 Taiwan 80-2 treatment: 500,000 spores per ml in 0.01% Tween 20 ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Martijn van de Mortel (for Steve Whitham). The equivalent experiment is GM36 at PLEXdb.] pathogen isolates: Mock - time: 12 hai(3-replications); pathogen isolates: Mock - time: 24 hai(3-replications); pathogen isolates: Mock - time: 72 hai(3-replications); pathogen isolates: Mock - time: 144 hai(3-replications); pathogen isolates: Mock - time: 216 hai(3-replications); pathogen isolates: Mock - time: 288 hai(3-replications); pathogen isolates: Taiwan 80-2 (vir) - time: 12 hai(3-replications); pathogen isolates: Taiwan 80-2 (vir) - time: 24 hai(3-replications); pathogen isolates: Taiwan 80-2 (vir) - time: 72 hai(3-replications); pathogen isolates: Taiwan 80-2 (vir) - time: 144 hai(3-replications); pathogen isolates: Taiwan 80-2 (vir) - time: 216 hai(3-replications); pathogen isolates: Taiwan 80-2 (vir) - time: 288 hai(3-replications); pathogen isolates: Hawaii 94-1 (avir) - time: 12 hai(3-replications); pathogen isolates: Hawaii 94-1 (avir) - time: 24 hai(3-replications); pathogen isolates: Hawaii 94-1 (avir) - time: 72 hai(3-replications); pathogen isolates: Hawaii 94-1 (avir) - time: 144 hai(3-replications); pathogen isolates: Hawaii 94-1 (avir) - time: 216 hai(3-replications); pathogen isolates: Hawaii 94-1 (avir) - time: 288 hai(3-replications)
2011-11-28 | E-GEOD-29740 | biostudies-arrayexpress
Project description:Experimental design: 2 genotypes: PI- (resistant USDA Plant Introduction (PI459025B) line containing SBR Rpp4 resistance gene) & Cultivar Williams that does not have a known SBR resistance gene 2 treatments: Soybean rust (Phakopsora pachyrhizi) isolate Hawaii 94-1 & mock infection 3 replications 6 time points: 12, 24, 72, 144, 216 and 288 hours after inoculation TOTAL: 72 Affymetrix GeneChip(R) Soybean Genome Arrays Mock treatment: 0.01% Tween 20 Hawaii 94-1 treatment: 500,000 spores per ml in 0.01% Tween 20 ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Steve Whitham. The equivalent experiment is GM37 at PLEXdb.]
2012-10-10 | GSE29741 | GEO
Project description:Four captive bottlenose dolpins housed in Kona, Hawaii, were serially sampled at approximately monthly intervals over the course of one year, in order to establish baseline information on the content and variation of the dolphin blood transcriptome.
2016-09-10 | GSE78770 | GEO
Project description:Whole blood was collected as part of monthly veterinary checkups of bottlenose dolphins housed at Dolphing Quest in Waikoloa, Hawaii, USA. Gene expression from 5 samples was analyzed for comparison to the blood transcriptome of the beluga whale.
2017-05-09 | GSE98627 | GEO
Project description:Brown algae (Phaeophyceae) are complex photosynthetic organisms with a very different evolutionary history to green plants, to which they are only distantly related. These seaweeds are the dominant species in rocky coastal ecosystems and they exhibit many interesting adaptations to these, often harsh, environments. The brown algae are also important because they are one of only a very small number of eukaryotic lineages that have evolved complex multicellularity. This work used whole genome tiling array approach to generate a comprehensive transcriptome map of the filamentous seaweed Ectocarpus siliculosus (Dillwyn) Lyngbye, a model organism for the brown algae. Keywords: high-resolution tiling array, brown algae, ectocarpus
2010-04-07 | GSE19912 | GEO
Project description:The Streptophyta include unicellular and multicellular charophyte green algae and land plants. Colonization of the terrestrial habitat by land plants was a major evolutionary event that has transformed our planet. So far lack of genome information on unicellular charophyte algae hinders our understanding of the origin and the evolution from unicellular to multicellular life in Streptophyta. This work reports the high-quality reference genome and transcriptome of Mesostigma viride, a single-celled charophyte alga with a position at the base of Streptophyta. There are abundant segmental duplications and transposable elements in M. viride, which contribute to a relatively large genome with high gene content compared to other algae and early diverging land plants. This work identifies the origin of genetic tools that multicellular Streptophyta have inherited and key genetic innovations required for evolution of land plants from unicellular aquatic ancestors. The findings shed light on the age-old questions of the evolution of multicellularity and the origin of land plants.
2019-11-21 | GSE123851 | GEO
Project description:The Streptophyta include unicellular and multicellular charophyte green algae and land plants. Colonization of the terrestrial habitat by land plants was a major evolutionary event that has transformed our planet. So far lack of genome information on unicellular charophyte algae hinders our understanding of the origin and the evolution from unicellular to multicellular life in Streptophyta. This work reports the high-quality reference genome and transcriptome of Mesostigma viride, a single-celled charophyte alga with a position at the base of Streptophyta. There are abundant segmental duplications and transposable elements in M. viride, which contribute to a relatively large genome with high gene content compared to other algae and early diverging land plants. This work identifies the origin of genetic tools that multicellular Streptophyta have inherited and key genetic innovations required for evolution of land plants from unicellular aquatic ancestors. The findings shed light on the age-old questions of the evolution of multicellularity and the origin of land plants.
2019-11-21 | GSE123850 | GEO
Project description:Brown algae (Phaeophyceae) are complex photosynthetic organisms with a very different evolutionary history to green plants, to which they are only distantly related. These seaweeds are the dominant species in rocky coastal ecosystems and they exhibit many interesting adaptations to these, often harsh, environments. The brown algae are also important because they are one of only a very small number of eukaryotic lineages that have evolved complex multicellularity. This work used whole genome tiling array approach to generate a comprehensive transcriptome map of the filamentous seaweed Ectocarpus siliculosus (Dillwyn) Lyngbye, a model organism for the brown algae. Keywords: high-resolution tiling array, brown algae, ectocarpus The slides were hybridised with two, labelled samples: 1) a mixture of labelled cDNA corresponding to RNA samples from mature sporophytes and gametophytes and from immature sporophytes stressed either in high salt medium or by addition of hydrogen peroxide and 2) genomic DNA as a control.
2010-04-07 | E-GEOD-19912 | biostudies-arrayexpress
Project description:Ralstonia solanacearum species complex causes bacterial wilt in variety crops. Tomato cultivar Hawaii 7996 is a widely used resistance resource, however, the resistance is evaded by virulent strains with underlying mechanisms still unknown. Here, we reported that phylotype II strain ES5-1 can overcome Hawaii 7996 resistance. RipV2, a type Ⅲ effector specifically to phylotype Ⅱ strains, is vital in overcoming tomato resistance. RipV2, which encodes a novel E3 ubiquitin ligase, suppresses immune responses and TNL-mediated cell death. Tomato helper NLR N requirement gene 1 (NRG1), enhanced disease susceptibility 1 (EDS1), and senescence-associated gene 101b (SAG101b) were identified as RipV2 target proteins. RipV2 is essential for ES5-1 virulence in Hawaii 7996 but not in SlNRG1-silenced tomato, demonstrating SlNRG1 to be the bona fide RipV2 virulence target. Our results dissected the mechanisms of RipV2 in disrupting immunity and highlighted the importance of converged immune components in conferring bacterial wiltresistance.
2024-07-20 | PXD054061 | Pride