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ABSTRACT: Stimulation of extracellular electron transfer by the magnetic field and mechanisms revealed by transcriptome analysis

PROVIDER: PRJNA804964 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17965844_1.fastq.gz	Fastqsanger.gz
	SRR17965844_2.fastq.gz	Fastqsanger.gz
	SRR17965845_1.fastq.gz	Fastqsanger.gz
	SRR17965845_2.fastq.gz	Fastqsanger.gz
	SRR17965846_1.fastq.gz	Fastqsanger.gz

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Project description:Stimulation of extracellular electron transfer by the magnetic field and mechanisms revealed by transcriptome analysis

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Magnetic Field Suppresses Non-Small Cell Lung Cancer via RSRP1-Mediated Secretory Signaling Axis [KO]

Project description:Magnetic field therapy holds promise for tumor treatment, yet its underlying mechanisms remain controversial. The traditional ROS theory fails to fully explain the observed magnetic field-induced inhibition. Building on prior findings, we identified the key target of magnetic field-mediated tumor suppression and established a magnetic inhibition model. Integrative multi-omics analysis (transcriptome + proteome) of 293T and A549 cells exposed to (5 mT, 20 Hz) magnetic fields pinpointed RSRP1 as a critical molecular target. We experimentally validated RSRP1's essential role using CRISPR-Cas9-mediated gene disruption and TET-on inducible expression systems. Proteomic analysis revealed that magnetic field exposure (5 mT, 20 Hz) induced >200-fold upregulation of RSRP1 protein in both non-tumorigenic 293T and tumorigenic A549 cells. CRISPR-Cas9-mediated RSRP1 knockout completely abolished A549 cells' responsiveness to magnetic fields. Notably, restoring RSRP1 expression via a doxycycline-inducible system reinstated magnetic field-induced secretion of inhibitory factors, with conditioned media from exposed cells significantly inhibiting naive tumor cell proliferation (p<0.05). Magnetic field (5 mT, 20 Hz) exposure transcriptionally upregulated RSRP1 endogenously, as confirmed by transcriptomic and qPCR analyses, while exogenously overexpressed RSRP1 via a doxycycline-inducible system remained unresponsive, indicating that magnetic regulation requires endogenous transcriptional control. This study establishes a "magnetic field→RSRP1→secretory factor→target response" cascade, providing a transformative framework for non-invasive tumor therapy.

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Transcription profiling by array of Clostridium acetobutylicum grown in open circuit (control) and closed circuit (current collecting) to observe the difference in extracellular electron transfer (EET) mechanisms.

Project description:Clostridium acetobutylicum was grown in open circuit (control) and closed circuit (current collecting) to observe the difference in extracellular electron transfer (EET) mechanisms. mRNA was extracted, amplified and used on Combimatrix custom arrays to obtain an expression profile. Analysis was performed using R.

2010-07-09 | E-MTAB-267 | biostudies-arrayexpress

Transcription profiling by array of Shewanella oneidensis was grown in open circuit (control) and closed circuit (current collecting) to observe the difference in extracellular electron transfer (EET) mechanisms.

Project description:Shewanella oneidensis was grown in open circuit (control) and closed circuit (current collecting) to observe the difference in extracellular electron transfer (EET) mechanisms. mRNA was extracted, amplified and used on Combimatrix custom arrays to obtain an expression profile. Analysis was performed using R.

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Transcription profiling by array of Pseudomonas aeruginosa and Enterococcus faecium coculture grown in open circuit (control) and closed circuit (current collecting) to observe the difference in extracellular electron transfer (EET) mechanisms.

Project description:Coculture Pseudomonas aeruginosa and Enterococcus faecium were grown in open circuit (control) and closed circuit (current collecting) to observe the difference in extracellular electron transfer (EET) mechanisms. mRNA was extracted, amplified and used on Combimatrix custom arrays to obtain an expression profile. Analysis was performed using R.

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