Ontology highlight
ABSTRACT:
PROVIDER: PRJNA806509 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR18003200_1.fastq.gz | Fastqsanger.gz | |||
| SRR18003200_2.fastq.gz | Fastqsanger.gz | |||
| SRR18003201_1.fastq.gz | Fastqsanger.gz | |||
| SRR18003201_2.fastq.gz | Fastqsanger.gz | |||
| SRR18003202_1.fastq.gz | Fastqsanger.gz |
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Project description:In recent years, avocados have gained popularity as a nutritive food worldwide. This trend is causing a rise in the production of this edible fruit, which is accompanied by a number of problems associated with monocultural practices. Despite massive economic gains, limited molecular and structural information has been generated about avocado ripening. Consequently, we conducted a comparative proteomics study on avocado peel and pulp during the postharvest shelf life using tandem mass tag synchronous precursor selection triple-stage mass spectrometry (TMT-SPS-MS3). We were able to identify 3161 and 1128 proteins in the peel and pulp, respectively. The peels exhibited a major over-accumulation of proteins associated with water deprivation and oxidative stress, along with abscisic acid biosynthesis. Ethylene, jasmonic acid, phenylpropanoid, and flavonoid biosynthesis pathways were activated. Structurally, we observed the accumulation of lignin and the reduction of the cuticular thickness, which coincides with the reduction in the levels of long-chain acyl-coenzyme A (CoA) synthetase (LACS) and a marginal increase in 10,16-dihydroxyhexadecanoic acid.
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Project description:Anthocyanins are high value plant antioxidants which are not present in the fruits of cultivated tomato. However, both the dominant gene Anthocyanin fruit (Aft) and the recessive gene atroviolacea (atv), introgressed into domesticated tomato from two different wild Solanum species, stimulate a limited anthocyanin pigmentation. Surprisingly, double mutant Aft/Aft atv/atv tomatoes are characterised by the presence of anthocyanins in the fruit peel, resulting in intensely purple pigmented fruit. We carried out a transcript profiling analysis using GeneChip® Tomato Genome Arrays, in order to identify differentially expressed genes when comparing wild type, Aft/Aft, atv/atv, and Aft/Aft atv/atv fruits. The expression pattern of several genes involved in the anthocyanin pathway was analyzed in detail. Among the fruit peel-associated differentially expressed transcripts, genes involved in phenylpropanoid pathway, cell wall composition, biotic and abiotic stress responses, sugar and hormone metabolism were overrepresented in Aft/Aft atv/atv. Transcriptomic analysis thus revealed that the activation of anthocyanin synthesis in tomato fruit was accompanied by a complex remodulation of gene expression, likely affecting important agronomic and merceological traits.
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