Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of formate on LN229 cells

ABSTRACT: Effect of formate on LN229 cells

PROVIDER: PRJNA806943 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR18017106.fastq.gz	Fastqsanger.gz
	SRR18017107.fastq.gz	Fastqsanger.gz
	SRR18017108.fastq.gz	Fastqsanger.gz
	SRR18017109.fastq.gz	Fastqsanger.gz
	SRR18017110.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 9

Similar Datasets

Effect of formate on LN229 cells

Project description:We observed that high extracellular formate concentrations promote the in vitro invasiveness of glioblastoma (GBM) cells. To identify mechanistically, how formates promotes invasion we chose an unbiased approach of RNAseq.

2023-02-06 | GSE196706 | GEO

Effect of HSD52 overexpression on gene expression in LN229 glioma cells

Project description:In order to study the effect of HSD52 on the malignant progression and chemotherapy resistance of glioma, we examined the effect of HSD52 on the gene expression of brain glioma cells. We used lentiviral constructs to establish the LN229 cell line overexpressing HSD52. We subsequently performed gene expression profiling using RNA-seq data obtained from control LN229 cells and HSD52-overexpressing LN229 cells.

2025-05-29 | GSE246649 | GEO

Effect of HSD52 overexpression on gene expression in LN229 glioma cells

Project description:Effect of HSD52 overexpression on gene expression in LN229 glioma cells

| PRJNA1034052 | ENA

Comparative RNAseq of human WT and PARP3-/- LN229 cells

Project description:To identify the biological pathways regulated by PARP3, RNAseq of the WT and PARP3-/- LN229 human glioblastoma cells were performed. Samples were collected from subconfluent cells for total RNA isolation and RNA sequencing

2022-09-21 | GSE173801 | GEO

Transcriptomic alterations upon HSP90 inhibition in human LN229 and T98 glioblastoma cells in vitro

Project description:Purpose: Characterization of transcriptomic alterations upon HPS90 inhibition in human LN229 and T98G glioblastoma cells in vitro. Methods: LN229 and T98G glioblastoma cells were treated with 10 nM of the HSP90 inhibitor NW457 or the vehicle control for 24 h, and total RNA was subjected to 3' RNA sequencing. Results: Distinct transcriptomic alterations were observed, dominated by a transcriptional upregulation of the heat shock response. Conclusion: HSP90 inhibition resulted in distinct transcriptomic alterations and a clear upregulation of the heat shock response.

2021-04-07 | GSE164717 | GEO

Formate-induced phospho-signalling

Project description:The mitochondrial serine catabolism to formate induces a metabolic switch to a hypermetabolic state with high rates of glycolysis, purine synthesis and pyrimidine synthesis. While formate is a purine precursor it is not obvious link between formate and pyrimidine synthesis. Methods Here we combine phospho-proteome and metabolic profiling to determine how formate induces pyrimidine synthesis. We discover that formate induces CAD phosphorylation. Mechanistically formate induces mTORC1 activity as quantified by S6K1 phosphorylation, which is known to phosphorylate CAD and increase its enzymatic activity. Treatment with the allosteric mTORC1 inhibitor rapamycin abrogates CAD phosphorylation and pyrimidine synthesis induced by formate. We conclude that formate activates mTORC1 and induces pyrimidine synthesis via increasing CAD activity.

2020-09-28 | PXD019347 | Pride

Transcriptome sequence of LN229 cells with UM-164 treatment

Project description:Transcriptome sequence of LN229 cells with UM-164 treatment

| PRJNA881608 | ENA

Comparative RNAseq of human WT and PARP3-/- LN229 cells

Project description:Comparative RNAseq of human WT and PARP3-/- LN229 cells

| PRJNA727084 | ENA

A Metal-Dependant Formate Dehydrogenase Advances the Formate Bio-economy

Project description:In this manuscript a multi-subunit metal dependent FDH is functionally expressed in a strain of E. coli engineered to assimilate all energy and biomass from formate. The replacement of the previously described FDH with this new, faster FDH allows for improved growth parameters rivaling natural formatotrophs for the first time. This new, robsulty growing formatotrophic E. coli was engineered to produce mevalonate and achieved the highest reported titer for any bioproduct from formate in fed batch mode. Finally the authors explored bioproducion from electrochemically-derived and lignin-derived formate, demonstrating the first example of a bioproduct produced using liginin-derived formate as a feedstock.

2025-06-06 | PXD062361 | Pride

Steady-state samples of Methanococcus maripaludis MM901 grown on formate under hydrogen limited conditions

Project description:Hydrogenotrophic methanogenic Archaea are defined by a H2 requirement for growth. Despite this requirement, many hydrogenotrophs are also capable of growth with formate as an electron donor for methanogenesis. Hydrogenotrophs respond to H2 starvation both phenotypically and at the level of gene expression; however, the responses during growth on formate have not been characterized. Here we report that during continuous culture of Methanococcus maripaludis under defined nutrient conditions, growth yields relative to methane production decreased markedly with either H2 excess or formate excess, suggesting that energy spilling occurs. Using microarray analysis, we show that the expression of genes encoding F420-dependent steps of methanogenesis, including one of two formate dehydrogenases, increased with H2 starvation, but with formate occurred at high levels regardless of limitation or excess. One gene, encoding H2-dependent methylene-tetrahydromethanopterin dehydrogenase, decreased in expression with either H2 limitation or formate limitation. Expression of genes for the second formate dehydrogenase, molybdenum-dependent formylmethanofuran dehydrogenase, and molybdenum transport increased specifically with formate limitation. Of the two formate dehydrogenases, only the first could support growth on formate in batch culture where formate was in excess.

2013-05-03 | GSE42111 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data