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Generation of transcriptional regulatory network of Lgr5+ small and large intestinal stem cells from mouse [scRNA-seq]

ABSTRACT: Generation of transcriptional regulatory network of Lgr5+ small and large intestinal stem cells from mouse [scRNA-seq]

PROVIDER: PRJNA807706 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18054197_1.fastq.gz	Fastqsanger.gz
	SRR18054197_2.fastq.gz	Fastqsanger.gz

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Generation of transcriptional regulatory network of Lgr5+ small and large intestinal stem cells from mouse

Project description:Generation of transcriptional regulatory network of Lgr5+ small and large intestinal stem cells from mouse

| PRJNA807704 | ENA

Generation of transcriptional regulatory network of Lgr5+ small and large intestinal stem cells from mouse [RNA-seq]

Project description:Generation of transcriptional regulatory network of Lgr5+ small and large intestinal stem cells from mouse [RNA-seq]

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Generation of transcriptional regulatory network of Lgr5+ small and large intestinal stem cells from mouse [ATAC-seq]

Project description:Generation of transcriptional regulatory network of Lgr5+ small and large intestinal stem cells from mouse [ATAC-seq]

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Generation of transcriptional regulatory network of Lgr5+ small and large intestinal stem cells from mouse [scRNA-seq]

Project description:The goal of this study is to survey transcriptional machinery in intestinal stem cells from small and large intestine of mice. We established intestinal stem cell specific gene-regulatroy network to understand regional identity.

2023-04-20 | GSE196915 | GEO

Generation of transcriptional regulatory network of Lgr5+ small and large intestinal stem cells from mouse [RNA-seq]

2023-04-20 | GSE196914 | GEO

Generation of transcriptional regulatory network of Lgr5+ small and large intestinal stem cells from mouse [ATAC-seq]

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Generation of transcriptional regulatory network of Lgr5+ small and large intestinal stem cells from mouse

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-04-20 | GSE196916 | GEO

Lgr5+tuft marker+ intestinal tumor cells

Project description:We previously identified Dclk1, a tuft cell marker, marks tumor stem cells (TSCs) in mouse intestinal tumors. In this study, we have identified IL17RB as a cell surface marker distinctively expressed by Dclk1+ tuft-like tumor cells in mouse intestinal tumors. Using this tuft cell marker, we compared and analyzed the transcriptome of Lgr5-tuft marker-, Lgr5+tuft marker-, Lgr5-tuft marker+, and Lgr5+tuft marker+ tumor cells. These analyses revealed that tuft-like tumor cells in the intestinal tumors comprise two distinct subsets: highly differentiated tuft-like tumor cells (Lgr5-tuft marker+ cells) and tuft-like tumor cells with TCS potential (Lgr5+tuft marker+ cells).

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Small nucleolar RNA Snora61 drives self-renewal of small intestinal stem cells via initiation of Lgr5 transcription

Project description:Intestinal epithelium relies on intestinal stem cells (ISCs) for rapid and precise tissue replenishment to maintain gut normal function. The self-renewal maintenance of ISCs is finely regulated by multiple stemness factors and signaling pathways. However, the transcription mechanisms of some key stemness factors remain poorly understood. Here we identified that small nucleolar RNA Snora61 is highly expressed in ISCs. Snora61 is mainly distributed in the nucleoplasm. Snora61 knockout impairs ISC self-renewal and intestinal regeneration. Mechanistically, Snora61 binds to the promoter region of Lgr5 gene and engages with RNA-binding protein RBMX to recruit HMGB2 onto Lgr5 promoter, leading to Lgr5 transcription and expression. Snora61 promotes the self-renewal of small intestinal stem cells, which in turn enhances the proliferation of downstream differentiated cells, thereby contributing to the maintenance of intestinal homeostasis. Conversely, Snora61 knockout causes reduced LGR5 expression. Deletion of Lgr5 with Snora61 displays more severely impaired ISC self-renewal and intestinal regeneration. Our findings reveal a novel regulatory mechanism of Lgr5 transcription underlying ISC self-renewal maintenance.

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Mex3a Marks a Slowly Dividing Subpopulation of Lgr5+ Intestinal Stem Cells

Project description:The intestinal epithelium is continuously regenerated by highly proliferative Lgr5+ intestinal stem cells (ISCs). The existence of a population of quiescent ISCs has been suggested yet its identity and features remain controversial. Here we describe that the expression of the RNA-binding protein Mex3a labels a subpopulation of Lgr5+ cells that divide less frequently and contribute to regenerate all intestinal lineages with slow kinetics. Single cell transcriptomic analysis revealed two classes of Lgr5-high cells, one of them defined by the Mex3a-expression program and by low levels of proliferation genes. Lineage tracing experiments show that large fraction of Mex3a+ cell population is continuously recalled into the rapidly dividing self-renewing ISC pool in homeostatic conditions. Chemotherapy and radiation target preferentially rapidly dividing Lgr5+ cells but spare the Mex3a-high/Lgr5+ population, which helps sustain the renewal of the intestinal epithelium during treatment.

2017-03-14 | GSE90856 | GEO

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