Effects of excessive FSH dose on granulosa cell, cumulus cell and oocyte transcriptomes in small ovarian reserve heifers
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ABSTRACT:
PROVIDER: PRJNA809004 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR18090400.fastq.gz | Fastqsanger.gz | |||
| SRR18090401.fastq.gz | Fastqsanger.gz | |||
| SRR18090402.fastq.gz | Fastqsanger.gz | |||
| SRR18090403.fastq.gz | Fastqsanger.gz | |||
| SRR18090404.fastq.gz | Fastqsanger.gz |
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Project description:To evaluate the mechanisms by which excessive FSH doses during ovarian stimulation are detrimental to ovarian follicle function and assisted reproduction outcomes, small ovarian reserve (SOR) heifers were subjected to ovarian stimulation with either a standard, 70 IU, or excessive, 210 IU, dose of commercial porcine FSH (cpFSH) preparation, per injection. The ovaries were recovered at the end of the ovarian stimulation protocol, consisting of eight cpFSH injections at 12 h intervals. In excessive dose treated heifers, heterogeneity was observed with regards to follicle phenotype including cumulus cell-oocyte complex (COC) morphology and intrafollicular steroid hormone milieu. In contrast, standard-dose treated heifers exhibited one predominant follicle phenotype which was selected as the control follicle type for all comparisons herein. RNA-seq analyses of granulosa, cumulus cells and the oocyte from individual follicles of these defined phenotypes was used to identify changes in the transcriptomes of these cell types in excessive dose treated individuals.
2022-08-10 | GSE197116 | GEO
Project description:Study the effect of recombinant human FSH supplementation in the in vitro maturation medium for an incubation of 6 hrs on the transcriptomics of bovine cumulus cells. 3 biological replicates of cumulus cells derived from cumulus oocyte complexes (COCs) supplemented with FSH (CC-FSH) vs. cumulus cells without FSH (Ct-6H, REFERENCE) were contrasted. Each replicate was subjected to a dye-swap procedure.
2015-07-01 | E-GEOD-67970 | biostudies-arrayexpress
Project description:Changes in gene expression induced by FSH in cumulus cells treated with adenosine using Agilent EmbryoGENE microarray slides. Bovine cumulus-oocyte complexes in vitro-cultured for 6 h using adenosine. The effect of FSH was compared with control as non-treated cumulus-oocyte complexes. After culture, COC were were washed and cumulus cells were used for microarray analysis
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Project description:Study the effect of recombinant human FSH supplementation in the in vitro maturation medium for an incubation of 6 hrs on the transcriptomics of bovine cumulus cells.
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Project description:Follicle stimulating hormone (FSH) and epidermal growth factor (EGF) are currently used on cumulus-oocyte complexes to mimic the luteinizing hormone surge in vitro and induce oocyte maturation and cumulus expansion. We have previously shown that addition of exogenous recombinant growth differentiation factor 9 (GDF9) during oocyte in vitro maturation led to an improvement of oocyte quality, as shown by an increased blastocyst percentage and fetal survival. Our objective was to characterize the effect of FSH/EGF and GDF9 treatments on mouse cumulus cells expression profile by microarray analysis.
2011-12-10 | GSE19327 | GEO
Project description:Follicle stimulating hormone (FSH) and epidermal growth factor (EGF) are currently used on cumulus-oocyte complexes to mimic the luteinizing hormone surge in vitro and induce oocyte maturation and cumulus expansion. We have previously shown that addition of exogenous recombinant growth differentiation factor 9 (GDF9) during oocyte in vitro maturation led to an improvement of oocyte quality, as shown by an increased blastocyst percentage and fetal survival. Our objective was to characterize the effect of FSH/EGF and GDF9 treatments on mouse cumulus cells expression profile by microarray analysis. Cumulus-oocyte complexes (COCs) were recovered from 21 to 26 day old female 129/SV mice, 44 hours post equine chorionic gonadotropin treatment (eCG (5 IU)). For the microarray experiment whole COCs were treated with 293H control medium (0.125% v/v), with 20 ng/ml GDF9 or with a combination of 50 mIU/ml FSH and 10 ng/ml EGF. After 8 hours of in vitro maturation, COCs were denuded by gentle pipetting, the oocytes were removed and the cumulus cells centrifuged and extracted RNA analysed by microarray.
2011-12-10 | E-GEOD-19327 | biostudies-arrayexpress
Project description:Background: The gonadotropin-induced resumption of oocyte meiosis in preovulatory follicles is preceded by expression of epidermal growth factor (EGF)-like peptides, amphiregulin (AREG) and epiregulin (EREG), in mural granulosa and cumulus cells. Both the gonadotropins and the EGF-like peptides possess the capacity to stimulate resumption of oocyte meiosis in vitro via activation of a broad signaling network in cumulus cells. To better understand the rapid genomic actions of gonadotropins (FSH) and EGF-like peptides, we analyzed transcriptomes of cumulus cells at 3 h after their stimulation. Methods: We hybridized aRNA from cumulus cells to a pig oligonucleotide microarray and compared the transcriptomes of FSH- and AREG/EREG-stimulated cumulus cells with untreated control cells and vice versa. The identified over- and underexpressed genes were subjected to functional genomic analysis according to their molecular and cellular functions. The expression pattern of 50 selected genes with a known or potential function in ovarian development was verified by real-time qRT-PCR. Results: Both FSH and AREG/EREG increased the expression of genes associated with regulation of cell proliferation, cell migration, blood coagulation and extracellular matrix remodeling. FSH alone induced the expression of genes involved in inflammatory response and in the response to reactive oxygen species. Moreover, FSH stimulated the expression of genes closely related to some ovulatory events either exclusively or significantly more than AREG/EREG (AREG, ADAMTS1, HAS2, TNFAIP6, PLAUR, PLAT, and HSD17B7). In contrast to AREG/EREG, FSH also increased the expression of genes coding for key transcription factors (CEBPB, FOS, ID1/3, and NR5A2), which may contribute to the differing expression profiles of FSH- and AREG/EREG-treated cumulus cells. Conclusions: The impact of FSH on cumulus cell gene transcription was higher than the impact of EGF-like factors in terms of the number of cell functions affected as well as the number of over- and underexpressed genes. Both FSH and EGF-like factors overexpressed genes involved in the post-ovulatory switch in steroidogenesis and tissue remodelling. However, FSH was remarkably more efficient in the up-regulation of several specific genes essential for ovulation of matured oocytes and also genes that been reported to play an important role in maturation of cumulus-enclosed oocytes in vitro.
2015-09-30 | GSE64858 | GEO
Project description:Transcriptomes of mouse mural granulosa cells were sequenced to identify transcripts expressed in mural granulosa cells of ovaries. Moreover, transcriptomes of cumulus cells were compared between those of young (2 month-old) and old mice (10 month-old) to assess the effects of ageing on cumulus cells. In addition, transcriptomes of cumulus-oocyte complexes were compared between DBA/2 and (C57BL/6 x DBA/2)F1 mice to assess the strain differences.
2016-04-15 | GSE80326 | GEO
Project description:Ovaries from cyclic sows were derived from a local slaughterhouse and transported to the laboratory at 27-33ºC within 2 hours after death. After removal of surrounding tissue, the ovaries were rinsed with water (27-33ºC) and submersed in PBS containing 100U/ml penicillin and 100µg/ml streptomycin (Gibco, Breda, The Netherlands) at 37ºC. Granulosa cells and oocytes were aspirated with a 26G needle from healthy, 3-6 mm follicles. Follicular health was determined according to previously described criteria (28). Harvested cells were pooled and the cell suspension was washed twice by centrifugation at room temperature in McCoys 5a medium with glutamine (Invitrogen, Breda, The Netherlands) supplemented with 100U/ml penicillin, 100µg/ml streptomycin, 0.1% BSA (Fluka, Zwijndrecht, The Netherlands), 2.5µg/ml transferrin and 4ng/ml sodium selenite. Oocytes were removed from the cell suspension by filtration through a 70µm cell sieve (Becton en Dickinson, Alphen a/d Rijn, The Netherlands). The number of viable GCs was estimated by trypan blue exclusion. Granulosa cells were cultured in serum free medium as previously described by Picton and colleagues (26). For co-culture experiments cumulus oocyte complexes (COC) were aspirated from 3-6mm follicles. Cell debris and COC were allowed to settle at the bottom of the tube and the supernatant was discarded. Cumulus oocyte complexes were washed in TL-HEPES and subsequently selected by morphological appearance (dark cytoplasm and at least 2 layers of cumulus cells). The COC were transferred to wash medium containing 0.1U/l FSH, 100mg/l long R3-IGF-1, 10mg/l insulin (all from Sigma Aldrich, Zwijndrecht, The Netherlands) and 100mg/l testosterone (Fluka, Zwijndrecht, The Netherlands). Granulosa cells, 7.5*106 per well of a 6 wells plate, and COC, 80-100, were co-cultured in 3.5ml medium per well of a 6 wells plate. Cells were cultured in a humidified atmosphere at 37ºC and 5% CO2 for 48 hrs.
2010-03-31 | E-MEXP-1768 | biostudies-arrayexpress
Project description:FSH signaling is well known to lead to steroidogenesis, cellular proliferation, and follicular growth. The development of global transcriptomic allows to expand our understanding of the FSH effect on several additional functions of granulosa cells and to better understand how it controls or prevents several differentiation events in granulosa cells from medium size follicles
2016-05-01 | GSE74834 | GEO