Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Cystic fibrosis causes olfactory system defects by altering progenitor cell proliferation

ABSTRACT: Cystic fibrosis causes olfactory system defects by altering progenitor cell proliferation

PROVIDER: PRJNA809332 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR18099334.fastq.gz	Fastqsanger.gz
	SRR18099335.fastq.gz	Fastqsanger.gz
	SRR18099336.fastq.gz	Fastqsanger.gz
	SRR18099337.fastq.gz	Fastqsanger.gz
	SRR18099338.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Cystic fibrosis causes olfactory system defects by altering progenitor cell proliferation (scRNA-Seq)

Project description:A reduced sense of smell has been reported in people with cystic fibrosis (CF). These olfactory defects have largely been attributed to secondary manifestations of the disease, such as inflammation of the nasal mucosa. Here we show that CFTR, the gene responsible for CF, is expressed in proliferating olfactory human cells and that newborn CFTR null pigs display ultrastructural abnormalities in the olfactory epithelium and olfactory bulbs. In the absence of CFTR, olfactory sensory neurons still produce odor-evoked activity, but mutant animals display defective odor-guided suckling behavior after birth. Consistent with epithelial changes, we found a reduced expression of genes implicated in cell cycle and development in globose basal cells (GBCs), the neurogenic progenitor cells in the olfactory epithelium. Targeted sequencing revealed enhanced CFTR expression in the subpopulation of GBCs that is actively proliferating. Furthermore, CFTR loss caused a global reduction in the number of sensory neurons and altered olfactory receptors expression. Our findings highlight a previously unknown role of CFTR in olfactory system function by regulating progenitor cell proliferation in the olfactory epithelium.

2022-12-31 | GSE197189 | GEO

Cystic fibrosis causes olfactory system defects by altering progenitor cell proliferation (Bulk RNA-Seq)

2022-12-31 | GSE197184 | GEO

Cystic fibrosis causes olfactory system defects by altering progenitor cell proliferation (scRNA-Seq)

Project description:Cystic fibrosis causes olfactory system defects by altering progenitor cell proliferation (scRNA-Seq)

| PRJNA809339 | ENA

Cystic fibrosis causes olfactory system defects by altering progenitor cell proliferation (Bulk RNA-Seq)

Project description:Cystic fibrosis causes olfactory system defects by altering progenitor cell proliferation (Bulk RNA-Seq)

| PRJNA809342 | ENA

Cystic fibrosis causes olfactory system defects by altering progenitor cell proliferation

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-12-31 | GSE197187 | GEO

SARS-CoV-2 infection causes transient olfactory dysfunction in mice

Project description:SARS-CoV-2 infection causes a wide spectrum of clinical manifestations in human, and olfactory dysfunction represents as one of the most predictive and common symptoms in COVID-19 patients. However, the underlying mechanism how SARS-CoV-2 infection leads to olfactory disorders remains elusive. Herein we demonstrate intranasal inoculation of SARS-CoV-2 induces robust viral replication in the olfactory epithelium (OE), not olfactory bulb (OB), resulting in transient olfactory dysfunction in humanized ACE2 mice. The sustentacular cells and Bowman's gland cells in the OE were identified as the major target cells of SARS-CoV-2 before the invasion into olfactory sensory neurons. Remarkably, SARS-CoV-2 infection triggers massive cell death and immune cell infiltration, and directly impairs the uniformity of OE structure. Combined transcriptomic and quantitative proteomic analyses reveal the induction of antiviral and inflammatory responses, as well as the downregulation of olfactory receptor (OR) genes in the OE from the infected animals. Overall, our mouse model recapitulates the olfactory dysfunction in COVID-19 patients, and provides critical clues to understand the physiological basis for extrapulmonary manifestations of COVID-19.

2021-06-22 | PXD026854 |

Parental copper stress induces offspring developmental defects by altering DNA methylation in zebrafish

Project description:Parental copper stress induces offspring developmental defects by altering DNA methylation in zebrafish

| PRJNA758396 | ENA

An epigenetic repressor TRIM66 dictates monogenic olfactory receptor expression, neural activity, and olfactory behavior

Project description:Olfactory receptor gene choice is an extremely intricate example of monogenic and monoallelic expression, where one out of over 1,000 receptor genes is transcribed in each olfactory sensory neuron. This process involves expression of multiple olfactory receptor genes in immature neurons, followed by silencing of all but one receptor genes during maturation. However, the molecular identity of the repressors remains mysterious. Here, we discover TRIM66 as a key repressor. Multiple receptor genes are retained at low levels in most single mature OSNs after deletion of Trim66, leading to decreased expression of the vast majority of olfactory receptor genes. Mechanistically, TRIM66 can bind to, assembly, and repress olfactory receptor enhancers, thereby silencing extra olfactory receptor genes. Functionally, deletion of Trim66 leads to severe defects in the olfactory information processing and innate olfactory behaviors. Our study provides the missing link in understanding the transition from polygenic to monogenic olfactory receptor expression.

2025-09-17 | GSE277322 | GEO

Transcription profiling of olfactory epithelia isolated from wild type and KLF7 null mice to identify potential KLF7 target genes in olfactory sensory neurons.

Project description:KLF7 null mice show profound axonal growth defects in the olfactory epithelium. The goal of this study was the identification of potential KLF7 target genes in olfactory sensory neurons. Experiment Overall Design: Olfactory epithelia were isolated from 3 wildtype and 3 mutant 1 day old pups and the RNA isolated, labeled and hybridized to one chip each.

2007-12-01 | E-GEOD-3595 | biostudies-arrayexpress

Identification of potential KLF7 target genes in olfactory sensory neurons

2006-06-10 | GSE3595 | GEO