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Millimeter Waves Modulate the Transcriptome in Human Fibroblasts

ABSTRACT: Millimeter Waves Modulate the Transcriptome in Human Fibroblasts

PROVIDER: PRJNA809696 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18114310_1.fastq.gz	Fastqsanger.gz
	SRR18114310_2.fastq.gz	Fastqsanger.gz
	SRR18114311_1.fastq.gz	Fastqsanger.gz
	SRR18114311_2.fastq.gz	Fastqsanger.gz
	SRR18114312_1.fastq.gz	Fastqsanger.gz

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Millimeter Waves Modulate the Transcriptome in Human Fibroblasts

Project description:Millimetre-wave (MMW) frequencies of the electromagnetic spectrum are increasingly being adopted in modern technologies including mobile communications, networking, and security screening. These frequencies are absorbed by the outer layers of the skin, however the biological effects are not well characterised. Thus, as emerging technologies increase human exposure, understanding the effects of MMWs on biological material and cell biology in the skin is critical in determining safe exposure levels. Here, we report on the exposure of primary human dermal fibroblasts to MMWs; we find that the radiation triggers genomic and transcriptomic alterations. In particular, repeated 60 GHz MMW exposures at 2.6 mW cm-2, 46.8 J cm-2 doses, trigger a physiological response in human fibroblasts distinct from conventional cytokine-induced stimulation. Our results show that high dose MMWs induce non-thermal transcriptomic alterations with simultaneous changes in DNA structural dynamics including formation of G-quadruplex and i-motif secondary structures, but not DNA damage.

2022-04-15 | GSE197295 | GEO

Transcriptional landscape of human keratinocyte models exposed to 60-GHz millimeter-waves

Project description:Transcriptional landscape of human keratinocyte models exposed to 60-GHz millimeter-waves

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Waves of cell death modulate the abundance and maturation of human retinal ganglion cells

Project description:Waves of cell death modulate the abundance and maturation of human retinal ganglion cells

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Waves of cell death modulate the abundance and maturation of human retinal ganglion cells

Project description:Retinal ganglion cells (RGCs) are the projection neurons that transmit visual information from the retina to the brain via the optic nerve. A substantial proportion of RGCs are eliminated by programmed cell death during development to regulate their final number, yet how cell death impacts RGC development in human retinas remains poorly understood. Here, we characterized the timing and cell-type-specificity of cell death in human fetal retinas and retinal organoids. We identified two waves of apoptosis: an early wave targeting retinal neuronal precursors and a late wave affecting RGCs and other neurons. In addition to these two waves of apoptosis, organoids displayed a distinct wave of necrosis in the innermost core region that eliminates nearly all RGCs during long-term culture. To investigate the functions of the apoptotic waves during retinal development, we differentiated BAX/BAK double mutant organoids that are deficient for apoptosis. In these mutant organoids, RGC survival was improved, RGC neurogenesis and maturation were delayed, and RGCs were lost through a compensatory increase in necrosis. Our data suggest that the waves of developmental apoptosis promote human RGC neurogenesis and maturation. Together, our results highlight the roles of cell death in human RGC development and the challenges in retinal organoid design. Overcoming these obstacles would improve the utility of retinal organoids for modeling optic neuropathies like glaucoma

2025-11-02 | GSE305194 | GEO

ADDITIVE EFFECTS OF MILLIMETER WAVES AND 2 DEOXYGLUCOSE CO-EXPOSURE ON THE HUMAN KERATINOCYTE TRANSCRIPTOME

Project description:To evaluate the effect of millimeter waves (MMW) exposure on the gene expression, we have employed whole genome microarray expression profiling. Neonatal primary Keratinocyte cells pooled from 3 healthy donors were exposed ex vivo, by 60.4 GHz-MMW, at an incident power density (IPD) of 20 mW/cm² (3 hours of treatment in athermic condition).No modification of keratinocyte transcriptome was observed. Considering that MMW could also impact the cell metabolism, we assessed then effect of glycolysis inhibitor 2 deoxyglucose treatment (2dG, 20 mM during 3 hours) in association with MMW co-exposure. 2dG treatment induces a high modification of the transcriptome (632 coding genes). Genes affected in their expression are linked with transcriptional repression, cellular communication and endoplasmic reticulum homeostasis. The MMW/2dG co-exposition induced a slight modification of cell transcriptome, which reflects the capacity of MMW to interfere with bioenergetic stress response. After RT-PCR validation, 6 genes (SOCS3, SPRY2, TRIB1, FAM46A, CSRNP1 and PPP1R15A) were confirmed as sensitive to MMW exposure during 2dG treatment.

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SK13_Wheat: Proteomic and biological analyses reveal the effect on growth under flooding stress of wheat irradiated with millimeter waves.

Project description:Proteomic and biological analyses reveal the effect on growth under flooding stress of wheat irradiated with millimeter waves

2024-08-29 | PXD031669 | JPOST Repository

SK11: Proteomic and biological analyses to reveal the effect on growth of chickpea irradiated with millimeter waves under flooding stress

Project description:Proteomic and biological analyses to reveal the effect on growth of chickpea irradiated with millimeter waves under flooding stress

2023-08-28 | PXD024561 | JPOST Repository

Transcriptional landscape of human keratinocyte models exposed to 60-GHz millimeter-waves

Project description:Purpose: The use of millimeter waves (MMW) will likely grow exponentially in the coming years due to their future utilization in high-speed telecommunications. The question of the possible biological effects of these frequencies has been raised, and in the present study, we aimed to explore, throw an innovating RNA sequencing approach known as Bulk RNA Barcoding sequencing (BRB-seq), the difference in gene expression under exposure. Methods: Tree main exposure scenario were explored. The first one aim to compare the cellular response of two primary culture of keratinocytes (HEK and NHEK) and one keratinocyte derivate cell line (HaCaT) exposed to MMW. The second scenario explore the dose effect of gradient of incident power density on gene expression in cell line. The last one explore the exposure time at the new exposure limit for the general population. Results: Our data shows that gene expression is quite different between the cellular models used, which validates the method used. However, the exposure scenarios demonstrates that the main effect was the heat effect associated with high power MMW. Some genes were also altered by exposure. Altered expression of 2 genes was confirmed with qRT–PCR. Conclusions: These results show that acute exposure to MMW has low effect on gene expression.

2024-06-20 | GSE234710 | GEO

Cancer-associated fibroblasts actively compress cancer cells and modulate mechanotransduction

Project description:Cancer-associated fibroblasts actively compress cancer cells and modulate mechanotransduction

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Multiple waves of transcriptome changes during extended hypoxic induction in human pulmonary microvascular endothelial cells

Project description:We profile the expression pattern of human pulmonary microvascular endothelial cells (HPMECs) at different time points of hypoxic stress. Through mRNA-seq, we identify functional waves of minor gene up-regulation at 8 and 24h hypoxia exposure followed by a massive wave of transcriptional activation after 48 hours. By weighted gene co-expression network analysis, we identify hub genes that likely play central roles in hypoxia transcription program. Strikingly, these hub genes included a prominent group of lincRNAs, suggesting non-coding RNAs may also have pivotal roles in the hypoxia regulatory circuit. HPMECs share a core hypoxia signature profile, but with some notably differences, indicating a portion of HPMECs hypoxia response is cell-specific. Collectively, our study comprehensive surveys the hypoxia transcriptome, and provides insight into the temporal dynamics of hypoxia transcriptional response. Time-course expression profiling of HPMECs exposed to hypoxia

2015-12-31 | E-GEOD-53510 | biostudies-arrayexpress

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