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Bioreactor for medium-chain fatty acids production from food waste extract

ABSTRACT: Biorefining food waste through the anaerobic conversion of endogenous lactate into caproate: a fragile balance between microbial substrate utilization and product inhibition.

PROVIDER: PRJNA809915 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18160955_1.fastq.gz	Fastqsanger.gz
	SRR18160955_2.fastq.gz	Fastqsanger.gz
	SRR18160956_1.fastq.gz	Fastqsanger.gz
	SRR18160956_2.fastq.gz	Fastqsanger.gz
	SRR18160957_1.fastq.gz	Fastqsanger.gz

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Bioreactor medium-chain fatty acids production from food waste extract

Project description:Direct Conversion of Food Waste Extract into Caproate: Metagenomics Assessment of Chain Elongation Process

| PRJNA872912 | ENA

Bioreactor medium-chain fatty acids production from food waste extract

Project description:Ecology of food waste chain-elongating microbiome

| PRJNA872917 | ENA

Project description:Fermented acid whey (lactate) to caproate

| PRJNA1111158 | ENA

Electrochemical valorization of waste activated sludge for short-chain fatty acids production.

Project description:A tremendous amount of waste activated sludge (WAS) ends up in landfilling even after a substantial retention time during anaerobic digestion. This leftover activated sludge is an organic-rich material with the high potential to produce value-added chemicals such as short chain fatty acids (SCFAs). In the present study, a novel electrochemical conversion of activated sludge (E-WAS) was carried out on the surface of non-precious electrodes (nickel, stainless-steel and copper) in alkaline media at low applied potential and temperature. Cyclic voltammetry showed that Cu (II)/Cu (III) and Ni (II)/Ni(III) redox couple catalyzed the WAS oxidation reaction to produce SCFAs and hydrogen. The results revealed that Cu(II)/Cu(III) has higher catalytic oxidation capability towards SCFAs. Yields of 48.7, 21.4, and 14.6 mg SCFAs per g of volatile solids were achieved by using copper, nickel and stainless-steel as working electrodes, respectively. Post analysis characterization techniques indicate that copper oxide films lead to WAS oxidation. Total volatile solid removal of 30% was obtained at 35°C and 1.65 V in 0.2 M NaOH after 2 h of operation in an electrochemical digestor with copper electrodes which is more efficient than a conventional alkaline treatment (24 h, 55%, 1M NaOH). Ammonia was produced as the by-product of E-WAS oxidation. The highest amount of ammonia (250 mg L-1) was obtained by using nickel as the working electrode after 2 h operation at 35°C and 1.35 V applied potential. The change in WAS morphology revealed that the copper oxide film is an effective electrocatalyst for WAS disinfection.

| S-EPMC9469876 | biostudies-literature

Vegetable oil enriched with n-caproate and n-caprylate via extractive lactate-based chain elongation

Project description:Vegetable oil enriched with n-caproate and n-caprylate via extractive lactate-based chain elongation

| PRJEB42300 | ENA

bioreactor for the anaerobic fermentation of food waste extract and medium chain fatty acids production

Project description:Metatranscriptomic analysis of bioreactor for the anaerobic fermentation of food waste extract and medium chain fatty acids production

| PRJNA768991 | ENA

Direct Conversion of Food Waste Extract into Caproate: Metagenomics Assessment of Chain Elongation Process.

Project description:In a circular economy strategy, waste resources can be used for the biological production of high added-value substances, such as medium chain fatty acids (MCFAs), thus minimising waste and favouring a sustainable process. This study investigates single-stage fermentation processes for the production of MCFAs in a semi-continuous reactor treating the extract of real food waste (FW), without the addition of external electron donors. Two sequential acidogenic fermentation tests were carried out at an organic loading rate (OLR) of 5 and 15 gCOD L-1d-1 with a hydraulic retention time of 4 days and pH controlled at 6 ± 0.2. The highest level of caproate (4.8 g L-1) was observed at OLR of 15 gCOD L-1d-1 with a microbiome mainly composed by lactate-producing Actinomyces, Atopobium, and Olsenella species and caproate-producing Pseudoramibacter. Metagenomic analysis revealed the presence of key enzymes for the production of lactate, such as lactate dehydrogenase and pyruvate ferredoxin oxidoreductase, as well as several enzymes involved in the reverse β-oxidation pathway, thus suggesting the occurrence of a lactate-based chain elongation process.

| S-EPMC7915914 | biostudies-literature

Extracellular metaproteomics of medium-chain fatty acids produced from waste sludge without electronic donors

Project description:It has been demonstrated from the perspective of microorganisms that medium-chain fatty acids can be produced from excess sludge without electronic donors.

| PXD068284 |

intracellular metaproteomics of medium-chain fatty acids produced from waste sludge without electronic donors

Project description:It has been demonstrated from the perspective of microorganisms that medium-chain fatty acids can be produced from excess sludge without electronic donors.

| PXD068283 |

Branched chain amino acids impact health and lifespan indirectly via amino acid balance and appetite control

Project description:Elevated branched chain amino acids (BCAAs) are associated with obesity and insulin resistance. How long-term dietary BCAAs impact late-life health and lifespan is unknown. Here, we show that when dietary BCAAs are varied against a fixed, isocaloric macronutrient background, long-term exposure to high BCAA diets led to hyperphagia, obesity and reduced lifespan. These effects were not due to elevated BCAA per se or hepatic mTOR activation, but rather the shift in balance between dietary BCAAs and other AAs, notably tryptophan and threonine. Increasing the ratio of BCAAs to these AAs resulted in hyperphagia and was linked to central serotonin depletion. Preventing hyperphagia by calorie restriction or pair-feeding averted the health costs of a high BCAA diet. Our data highlight a role for amino acid quality in energy balance and show that health costs of chronic high BCAA intakes were not due to intrinsic toxicity; rather, to hyperphagia driven by AA imbalance.

2019-03-11 | GSE114855 | GEO

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