Ontology highlight
ABSTRACT:
PROVIDER: PRJNA809937 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR18122611_1.fastq.gz | Fastqsanger.gz | |||
| SRR18122611_2.fastq.gz | Fastqsanger.gz |
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Project description:Consolidated bioprocessing (CBP) represents a promising approach to convert biomass into valuable chemicals by integrating the hydrolysis of cellulose and hemicellulose with microbial fermentation in a single step. This study investigates the potential of CBP technique for the efficient conversion of lignocellulosic biomass into malic acid using the thermophilic fungus Myceliophthora thermophila. Transcriptomic analysis and genetic studies indicated that efficient degradation of lignin is crucial for improving the overall efficiency of CBP, as it facilitates access to cellulose and hemicellulose while enabling the utilization of lignin-derived compounds. The assay of lignin degradation products revealed that M. thermophila can channel main lignin-derived components into the central metabolic pathway for malic acid production. The combined enhancement of lignin degradation capabilities and optimization of the cellodextrin utilization pathway in M. thermophila resulted in 52.9% increase in malic acid production, from 36.3 g/L to 55.5 g/L directly from raw corncob. In a bioreactor, malic acid titer reached 151.2 g/L by one-step bioconversion of plant biomass, with the productivity and yield of 1.09 g/L/h and 0.55 g/g raw corncob. Following separation and purification, the purity of malic acid reached 99%. This study underscores the viability of CBP as a sustainable approach for biobased chemical production and emphasizes the importance of optimizing both lignin degradation and product synthesis pathways to maximize the efficiency of biomass conversion processes.
2026-08-19 | GSE281203 | GEO