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Artificial Immune Receptors as novel tools to engineer inflammation sensing regulatory T cells for cell therapy

ABSTRACT: Artificial Immune Receptors as novel tools to engineer inflammation sensing regulatory T cells for cell therapy

PROVIDER: PRJNA810473 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18145613.fastq.gz	Fastqsanger.gz
	SRR18145614.fastq.gz	Fastqsanger.gz
	SRR18145615.fastq.gz	Fastqsanger.gz
	SRR18145616.fastq.gz	Fastqsanger.gz
	SRR18145617.fastq.gz	Fastqsanger.gz

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Artificial Immune Receptors as novel tools to engineer inflammation sensing regulatory T cells for cell therapy

Project description:Engineered regulatory T cell (Treg) therapy is a promising strategy to treat patients suffering from inflammatory diseases and autoimmunity. In this study, we introduce a new class of synthetic sensors, named Artificial Immune Receptors (AIRs), for murine and human Treg cells that provide new features to this cell type. AIRs bind inflammatory ligands of the TNF-receptor superfamily and translate this environmental signal into a T cell receptor (TCR)-like program, leading to activation and proliferation of the Treg cells. In a preclinical graft-versus-host-disease model, AIR-Treg cells recognizing lymphotoxin-beta-receptor ligands protect significantly better than Treg cells with only a natural TCR repertoire. Expression and signaling of a human AIR construct in human Treg cells prove that the concept can be translated. Inflammation sensing AIR-Treg cells could be an off-the-shelf Treg cell therapy approach without the knowledge of a specific antigen driving the inflammatory process.

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RNA-seq data of mammalian cells treated with artificial transcription factors

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Coagulopathy in Malnourished Mice is Sexually Dimorphic and Regulated by Nutrient-sensing Nuclear Receptors

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The quorum sensing molecule 2'-aminoacetophenone activates plant cell-surface immune receptors

Project description:Quorum sensing (QS) enhances bacterial pathogenesis. How plants perceive QS signals remains elusive. Here, we report that Arabidopsis thaliana perceives 2'-aminoacetophenone (2’-AA), a QS-regulated volatile compound emitted from Pseudomonas aeruginosa, through cell-surface pattern recognition receptor (PRR) complexes that require BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE (BAK1). 2’-AA activates PRR-mediated immunity and enhances plant resistance to Pseudomonas pathogens. The 2’-AA-activated immunity displayed structural specificity of the elicitor and genetic specificity of the PRR complexes. 2’-AA induces in vivo protein interactions between BAK1 and its co-functioning PPRs, meanwhile the plant transcriptome responded to 2’-AA similarly as to flg22, a bacterial immunogenic elicitor that activates BAK1-dependent immunity. Hence bacterial QS signals can be sensed by plant cell-surface immune receptors.

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