Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Alfalfa (Medicago sativa L.) pho2 mutant plants hyperaccumulate phosphate [Iso-seq]

ABSTRACT: Alfalfa (Medicago sativa L.) pho2 mutant plants hyperaccumulate phosphate [Iso-seq]

PROVIDER: PRJNA810491 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR18145532_subreads.fastq.gz	Fastqsanger.gz
	SRR18145533_subreads.fastq.gz	Fastqsanger.gz
	SRR18145534_subreads.fastq.gz	Fastqsanger.gz
	SRR18145535_subreads.fastq.gz	Fastqsanger.gz
	SRR18145536_subreads.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Alfalfa (Medicago sativa L.) pho2 mutant plants hyperaccumulate phosphate

Project description:Alfalfa (Medicago sativa L.) pho2 mutant plants hyperaccumulate phosphate

| PRJNA810484 | ENA

Alfalfa (Medicago sativa L.) pho2 mutant plants hyperaccumulate phosphate [RNA-seq]

Project description:Alfalfa (Medicago sativa L.) pho2 mutant plants hyperaccumulate phosphate [RNA-seq]

| PRJNA810492 | ENA

Alfalfa (Medicago sativa L.) pho2 mutant plants hyperaccumulate phosphate [Iso-seq]

Project description:RNA-seq and Iso-seq reads used used for gene charaterization. Pacbio amplicon sequencing reads used for mutant characterization

2022-03-31 | GSE197480 | GEO

Alfalfa (Medicago sativa L.) pho2 mutant plants hyperaccumulate phosphate [RNA-seq]

Project description:RNA-seq and Iso-seq reads used used for gene charaterization. Pacbio amplicon sequencing reads used for mutant characterization

2022-03-31 | GSE197479 | GEO

MtIFS1-expressing alfalfa

Project description:We used an M. truncatula 16K oligonucleotide array to profile gene expression in the leaves of transgenic alfalfa plants expressing Medicago truncatula isoflavone synthase 1 (MtIFS1). RNA purified from leaves of MtIFS1-expressing lines C22 and B20 and vector control line VC11 was used to generate Cy5-labeled cDNA. RNA purified from a second vector control line, VB2, was used to generate Cy3-labeled reference cDNA. Three independently propagated cuttings of each line were used and a total of nine hybridizations were performed. Our results indicated that MtIFS1-expression does not significantly alter global gene expression in the leaves. Keywords = Medicago Keywords = isoflavone synthase Keywords = alfalfa Keywords: other

2005-07-20 | GSE2546 | GEO

Analysis of gene expression in leaves of Medicago truncatula plants inoculated with arbuscular mycorrhizal fungi and plants fertilized with phosphate

Project description:Arbuscular mycorrhizas (AM) are the most common symbiotic associations between plant’s root compartment and fungi. They can provide both nutritional benefit (mostly inorganic phosphate) leading to improved growth and non-nutritional benefits including defense responses to environmental cues throughout the host plant, which in return delivers carbohydrates to the symbiont. However how transcriptional changes occurring in AM leaves differ from those induced by phosphate fertilization is poorly understood. We investigated systemic changes in the leaves of mycorrhized Medicago truncatula compared to non-mycorrhized control plants, and in phosphate fertilized plants compared to control plants.

2016-04-26 | GSE80610 | GEO

Genotype- and tissue-specific microRNA profiles and their targets in three alfalfa (Medicago sativa L) accessions

Project description:Alfalfa (Medicago sativa L.) is a forage legume with significant agricultural value worldwide. MicroRNAs (miRNAs) are key components of post-transcriptional gene regulation and essentially control almost all aspect of plant growth and development. Although miRNAs have been reported from alfalfa but their expression profiles in different tissues and novel miRNAs as well as their targets have not been confirmed in this plant species. Therefore, we sequenced small RNAs in whole plantlets, shoots and roots of three different alfalfa genotypes (Altet-4, NECS-141 and NF08ALF06) to identify tissue-specific profiles. After comprehensive analysis using bioinformatics methods, we have identified 100 miRNA families, of which 21 belongs to the highly conserved families whereas the remaining 79 families are conserved between M. truncatula and M. sativa. The profiles of the six highly expressed conserved miRNA families (miR156, 159, 166, 319, 396, 398,) were relatively similar between the plantlets, roots and shoots of three genotypes. Contrastingly, the differenecs were robust between shoots and roots for miR160 and miR408 levels, which were low in roots compared to shoots. The study also has identified 17 novel miRNAs that also differed in their abundanecs between tissues of the alfalfa genotypes. Additionally, we have generated and analyzed the degradome libraries from three alfalfa genotypes that has confirmed 69 genes as targets for 31 miRNA families in alfalfa. The identification of conserved and novel miRNAs as well as their targets in different tissues of three genotypes not only enhanced our understanding of miRNA-mediated gene regulation in alfalfa but could also be useful for practical applications in alfalfa as well as related legume species.

2019-01-15 | GSE119460 | GEO

Medicago sativa cultivar:Alfalfa

Project description:Medicago sativa cultivar:Alfalfa Transcriptome or Gene expression

| PRJNA692570 | ENA

Alfalfa (Medicago sativa L.) pho2 mutant plants hyperaccumulate phosphate.

Project description:In this article, we describe a set of novel alfalfa (Medicago sativa L.) plants that hyper-accumulate Phosphate ion (Pi) at levels 3- to 6-fold higher than wild-type. This alfalfa germplasm will have practical applications reclaiming Pi from contaminated or enriched soil or be used in conservation buffer strips to protect waterways from Pi run-off. Hyper-accumulating alfalfa plants were generated by targeted mutagenesis of PHOSPHATE2 (PHO2) using newly created CRISPR/Cas9 reagents and an improved mutant screening strategy. PHO2 encodes a ubiquitin conjugating E2 enzyme (UBC24) previously characterized in Arabidopsis thaliana, Medicago truncatula, and Oryza sativa. Mutations of PHO2 disrupt Pi homeostasis resulting in Pi hyper-accumulation. Successful CRISPR/Cas9 editing of PHO2 demonstrates that this is an efficient mutagenesis tool in alfalfa despite its complex autotetraploid genome structure. Arabidopsis and M. truncatula ortholog genes were used to identify PHO2 haplotypes in outcrossing tetraploid M. sativa with the aim of generating heritable mutations in both PHO2-like genes (PHO2-B and PHO2-C). After delivery of the reagent and regeneration from transformed leaf explants, plants with mutations in all haplotypes of PHO2-B and PHO2-C were identified. These plants were evaluated for morphology, Pi accumulation, heritable transmission of targeted mutations, segregation of mutant haplotypes and removal of T-DNA(s). The Agrobacterium-mediated transformation assay and gene editing reagents reported here were also evaluated for further optimization for future alfalfa functional genomic studies.

| S-EPMC9157135 | biostudies-literature

Project description:Alfalfa (Medicago sativa L.) pho2 mutant plants hyperaccumulate phosphate

| PRJNA811622 | ENA