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Staphylococcus aureus strain:KS15 | isolate:Buffalo mastitic milk

ABSTRACT: Staphylococcus aureus strain:KS15 | isolate:Buffalo mastitic milk Genome sequencing

PROVIDER: PRJNA810579 | ENA |

REPOSITORIES: ENA

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Proteomic changes in the milk of water buffalo (Bubalus bubalis) with intramammary infection by Staphylococcus aureus and by non-aureus staphylococciProteomic changes in the milk of water buffalo (Bubalus bubalis) with intramammary infection by Staphylococcus aureus and by non-aureus staphylococci

Project description:The study reports a differential proteomic analysis of the Mediterranean buffalo milk to evaluate the changes induced by Staphylococcus spp. during a subclinical intramammary infection (IMI). A number of 12 quarter milk samples, 6 of which with somatic cell count (SCC) < 50,000 cells/mL and culture-negative, and the other 6 with SCC ˃ 3,000,000 cells/mL and culture-positive to Staphylococcus aureus (SAU, n=3), SAU, or non-aureus staphylococci (NAS, n=3) was selected. Samples were analyzed using a shotgun proteomics approach, based on filter-aided sample preparation (FASP) followed by LC-MS/MS and label-free analysis. Here, the largest buffalo milk protein dataset described so far was reported. Moreover, the results demonstrated that staphylococcal IMI mostly affected proteins involved in structural functions and in innate immune defense, with changes in their abundance that were generally more intense in SAU than in NAS samples. Further, an increase in the abundance of different cathelicidins was observed, as already reported for other animals with mastitis disease (1,2). (1) Addis MF, Pisanu S, Marogna G, Cubeddu T, Pagnozzi D, Cacciotto C, et al. Production and release of antimicrobial and immune defense proteins by mammary epithelial cells following Streptococcus uberis infection of sheep. Infect Immun. 2013;81: 3182–3197. (2) Addis MF, Tedde V, Dore S, Pisanu S, Puggioni GMG, Roggio AM, et al. Evaluation of milk cathelicidin for detection of dairy sheep mastitis. J Dairy Sci. Elsevier; 2016;99: 6446–6456. In conclusion, our results provide the first in depth characterization of buffalo milk proteins, describe the changes induced by SAU and NAS subclinical intramammary infection and suggest indications to reveal subclinical staphylococcal mastitis in buffalo by the milk proteome investigation.

2019-10-22 | PXD012355 | Pride

RNA-seq of Staphylococcus aureus human and bovine strains cultured in TSB and 50% milk media

Project description:The aim of this experiment was to understand the different transcriptomic profiles of Staphylococcus aureus strains when cultured in 50% milk media with TSB media as a control. Bovine S. aureus strains that are capable of clotting milk where compared to both bovine and human S. aureus strains unable to clot milk. All strains are from sequence type 97.

2024-09-04 | E-MTAB-14384 | biostudies-arrayexpress

Genomic comparison of vancomycin susceptible and vancomycin intermediate Staphylococcus aureus pairs

Project description:To determine if significant genomic changes are associated with the development of vancomycin intermediate Staphylococcus aureus, genomic DNA microarrays were performed to compare the initial vancomycin susceptible Staphylococcus aureus (VSSA) and a related vancomycin intermediate Staphylococcus aureus (VISA) isolate from five unique patients (five isolate pairs). Keywords: comparative genomic hybridization

2006-11-03 | GSE5047 | GEO

MicroRNA expression profiles of bovine milk exosomes in response to Staphylococcus aureus infection

Project description:Milk is an essential source of nutrients to all mammalian offspring, and is rich in a wide variety of immunological components. Recently reported miRNAs that are present in breast milk and are selectively packaged into the exosomes, a type of membrane vesicles, secreted by most cell types. These exosomal miRNAs could be actively delivered into recipient cells, and play a key role in the regulation of innate and adaptive immunity. In the present study we analyzed the lactation-related miRNA expression profiles in bovine milk exosomes at 2 days post-infection with Staphylococcus aureus (S. aureus) using a deep sequencing. Analyzing over 76.44 million sequencing reads, a total of 720 miRNAs including 303 high-confident miRNA candidates were identified by two different approaches. The top 10 highly expressed miRNAs accounted for approximately 80% of all aligned reads, with the remaining miRNAs showing much lower expression. Bta-miR-101, -142-5p, -183, -2285g-3p, -223 and -99a-5p were significantly differentially regulated in S. aureus infected milk compared to their uninfected controls. Target prediction of the differently expressed miRNAs revealed 219 potential target candidates, and 22 unique target genes identified by David Gene Ontology analysis were significantly related to host immune processes and inflammation. This new knowledge of milk miRNA expression in response to S. aureus infection significantly provides new and comprehensive information on milk miRNA composition in general as well as changes occurring during an infection.

2016-10-31 | GSE55144 | GEO

Staphylococcus aureus strain:UC01 | isolate:milk

Project description:Staphylococcus aureus strain:UC01 | isolate:milk Genome sequencing

| PRJNA1462172 | ENA

Staphylococcus aureus strain:UC02 | isolate:milk

Project description:Staphylococcus aureus strain:UC02 | isolate:milk Genome sequencing

| PRJNA1462174 | ENA

Staphylococcus aureus strain:UC08 | isolate:milk

Project description:Staphylococcus aureus strain:UC08 | isolate:milk Genome sequencing

| PRJNA1462185 | ENA

Staphylococcus aureus strain:NVC139 | isolate:milk

Project description:Staphylococcus aureus strain:NVC139 | isolate:milk Genome sequencing

| PRJNA1462231 | ENA

Staphylococcus aureus strain:UC04 | isolate:milk

Project description:Staphylococcus aureus strain:UC04 | isolate:milk Genome sequencing

| PRJNA1462182 | ENA

Staphylococcus aureus strain:NHF05 | isolate:milk

Project description:Staphylococcus aureus strain:NHF05 | isolate:milk Genome sequencing

| PRJNA1462225 | ENA

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