Project description:In this study, two small RNA libraries were constructed using dry period and peak lactation dairy goat mammary gland tissues and sequenced by the Illumina Solexa high-throughput sequencing system. A total of 346 conserved and 95 novel miRNAs were identified in the dairy goat. The expression of miRNAs was confirmed by qRT-PCR in nine tissues and the mammary gland during development cycles. In addition, several candidate miRNAs that may be involved in mammary gland development and lactation were found by the comparison of miRNA expression profiles among different tissue and developmental stages of the mammary gland. This study provides the identification and profile of miRNAs related to the biology of the mammary gland in the dairy goat. The identification of these miRNAs could contribute to understanding the molecular mechanisms of lactation physiology and the development of the mammary gland in the dairy goat.
Project description:Different doses of glucose were inused into dairy goat mammary gland. The mammary gland tissues were biopsied to analyze the changes of transcriptome responding to glucose infusion.
Project description:Five healthy Laoshan dairy goats (four years old, third lactation) from Qingdao Laoshan dairy goat primary farm (Shandong Province, China) were used. The mammary gland samples were collected surgically after general anaesthesia using Xylazine Hydrochloride injection solution (Huamu Animal Health Products Co., Ltd. China) at corresponding lactation stage, including early, peak and late lactations.
Project description:Next-generation sequencing (NGS) has revolutionized systems-based analysis of miRNA expression files. The goals of this study are to obtain the miRNA expression files in dairy goat mammary gland at early lactation (10th day after parturition) and early involution (5th day after forced weaning at late lactation, 218 day after parturition).
Project description:Fat metabolism is a complicated process regulated by a series of factors. microRNAs (miRNAs) are a class of negative regulator of proteins and play crucial roles in many biological processes, including fat metabolism. Although there have been some researches indicating that miRNAs could influence the milk fat metabolism through targeting some factors, little is known about the effect of miRNAs on goat milk fat metabolism. Here we utilized an improved miRNA detection assay, S-Poly(T), to profile the expression of miRNAs in the goat mammary gland in early-lactation and dry-lactation. Overall, we found 146 miRNAs expression changed between early-lactation and dry-lactation, with 81 up-regulation and 65 down-regulation, among which the miR-27a, miR-200a and miR-200c have been reported to be involved in the milk fat metabolism process.
Project description:This study aimed to characterize the whey proteome of colostrum, transitional milk, and mature milk from Murciano-Granadina goats, and to evaluate the use of transitional milk as a substitute for colostrum in feeding neonatal kids. Proteomic analysis identified a total of 1,385 proteins, with 736 in colostrum, 668 in transitional milk, and 625 in mature milk. Colostrum and transitional milk displayed a high degree of similarity between colostrum and transitional milk, particularly in proteins related to immune response and inflammation. In contrast, mature milk showed a shift toward proteins involved in metabolic and transport functions. To evaluate the practical application of transitional milk, newborn goat kids were fed either frozen colostrum (following standard farm protocol), freeze-dried colostrum, or freeze-dried transitional milk. All feeding strategies were well tolerated, with no adverse health effects, and resulted in similar growth performance. At 48 hours of life, serum IgG concentrations were 13.95 mg/mL in kids fed frozen colostrum, 17.36 mg/mL in those fed freeze-dried colostrum and 11.65 mg/mL in those fed freeze-dried transitional milk. Notably, kids fed freeze-dried transitional milk achieved satisfactory immunoglobulin levels, supporting its potential for passive immune transfer. These results highlight the biological value of transitional milk and support its use as a colostrum substitute in intensive farming systems, particularly when maternal colostrum is unavailable or insufficient. Furthermore, freeze-drying effectively preserved its functional properties, enabling convenient storage, handling, and administration. Overall, the successful use and preservation of transitional milk represent a sustainable approach to improving colostrum management, reducing waste, and increasing the efficiency of kid rearing in dairy goat production, in line with circular economy principles.
Project description:The mammary gland redeveloped to the pre-pregnancy state during involution, which shows that the mammary cells have the characteristics of remodeling. The rapidity and degree of mammary gland involution are different between mice and dairy livestock (dairy cows and dairy goats). However, the molecular genetic mechanism of miRNA in involution and remodeling of goat mammary gland has not yet been clarified. Therefore, this study carried out the RNA-sequencing of nonlactating mammary gland tissue of dairy goats in order to reveal the transcriptome characteristics of miRNA in nonlactating mammary tissues and clarify the molecular genetic mechanism of miRNA in mammary cell involution and remodeling.