Project description:Investigation of whole genome gene expression level changes in Xylella fastidiosa 9a5c biofilm, submitted to treatments with sub inhibitory and inhibitory concentrations of copper and tetracycline. A study of Xylella fastidiosa 9a5c was done using total RNA recovered from biofilm bacterial cells submitted to 3 or 7mM of CuSO4 or 100 or 800 µg/ml of tetracycline. Each chip measures the expression level of 2832 genes from Xylella fastidiosa 9a5c with thirteen 60-mer probe pairs (PM/MM) per gene, with five-fold technical redundancy.
Project description:Investigation of whole genome gene expression level changes in Xylella fastidiosa 9a5c biofilm, submitted to treatments with sub inhibitory and inhibitory concentrations of copper and tetracycline.
Project description:This is the study of the Heat Shock response of phytopathogenic bacteria Xylella fastidiosa. This series keeps the 25 minutes 40oC stimulus response (Aug 2005). Keywords: stress response; heat shock response
Project description:To investigate the role(s) of a cold shock protein homolog (Csp1) in plant pathogenic bacteria Xylella fastidiosa, we compared transcriptome profiles between wild type and a csp1 deletion mutant (Δcsp1) using long read Nanopore RNA sequencing.
Project description:Investigation of whole genome gene expression level changes in Xylella fastidiosa grown in minimal media XFM and XFM supplied with pectin or glucan (Host polysaccharides) , compared to cell grown in the complex media PWG. The cells grown in the minimal medium XFM supplied with host polysaccharides specially pectin are transmissible by the insect vector when delivered to the vector through artificial diet system. This does not happen with cells grown in the complex media. 4 (4 plex chips) study using total RNA recovered from 4 independents replicates for Xylella fastidiosa grown on PWG, XFM, XFM-glucan and XFM-pectin.
Project description:In a previous DNA-based microbiome study of grapevines in areas of Pierce’s Disease (PD) pressure, we determined that taxa belonging to the Pseudomonas and Achromobacter genera negatively associated with PD severity and titer of the causal agent, Xylella fastidiosa, leading us to hypothesize that these taxa suppress PD. Here we tested this hypothesis using two bacterial isolates from the grapevine endosphere, Pseudomonas viridiflava and Achromobacter vitis. We demonstrate that pre-treatment with these two isolates significantly reduced PD symptoms and X. fastidiosa titer, comparable to that of a known PD biocontrol agent, Paraburkholderia phytofirmans PsJN. We monitored early spatial transcriptional responses using genome-wide transcriptional profiling in vines that were pre-treated with the biocontrol strains and then inoculated with X. fastidiosa. We coupled this with phenotyping of internal tylose development and external disease symptoms and bacterial titer and determined that grapevines pre-treated with the biocontrols A. vitis and PsJN developed fewer tyloses and PD symptoms and underwent major transcriptional reprogramming in response to X. fastidiosa. These included up-regulation of genes in auxin- and ethylene-signaling pathways linked to tylose development. In contrast, P. viridiflava pre-treatment also resulted in a reduction of tyloses and PD symptoms but did not induce major transcriptional changes in vines, suggesting it likely has a direct inhibitory effect to X. fastidiosa through antibiosis. Using this data, we propose a model that incorporates timely and effective deployment of tyloses related to induction of ethylene and auxin pathways as a key factor in PD resistance.
Project description:Xylella fastidiosa is a Gram-negative plant pathogen responsible for severe diseases in a variety of economically important crops. A critical aspect of its pathogenicity is the production of outer membrane vesicles (OMVs). While some knowledge exists on the molecular cargo of X. fastidiosa (Xf)-OMVs, nucleic acid cargoes remain unknown. Since OMVs protect DNA from degradation during transfer, vesiduction increases the efficiency of HGT, aiding bacterial evolution and adaptation. In order to understand if this mechanism is relevant for Xf, we sequenced DNA extracted from OMVs and compared it to DNA extracted from whole bacterial cells.
