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A nucleolar condensin-recruiting complex that regulates yeast chromosome III structure

ABSTRACT: A nucleolar condensin-recruiting complex that regulates yeast chromosome III structure

PROVIDER: PRJNA813880 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18272400.fastq.gz	Fastqsanger.gz
	SRR18272401.fastq.gz	Fastqsanger.gz
	SRR18272402.fastq.gz	Fastqsanger.gz
	SRR18272403.fastq.gz	Fastqsanger.gz
	SRR18272404.fastq.gz	Fastqsanger.gz

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A nucleolar condensin-recruiting complex that regulates yeast chromosome III structure [ChIP-Seq]

Project description:A nucleolar condensin-recruiting complex that regulates yeast chromosome III structure [ChIP-Seq]

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A nucleolar condensin-recruiting complex that regulates yeast chromosome III structure [ChIP-Seq]

Project description:Purpose: To identify cohibin-dependent condensin binding sites throughout the yeast genome. Conclusions: This ChIP-seq experiment showed that the condensin subunit, Brn1, was depleted from the RDT1 promoter in an lrs4Δ mutant strain. The experiment also showed that the Lrs4 subunit of the cohibin complex is highly enriched at the RDT1 promoter on chromosome III.

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A nucleolar condensin-recruiting complex that regulates yeast chromosome III structure

Project description:This SuperSeries is composed of the SubSeries listed below.

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Project description:Faithful chromosome segregation requires packaging of the genome on both global and local scales. Condensin plays a crucial role at pericentromeres to resist spindle forces and ensure the oriented attachment of kinetochores to microtubules at mitosis. Here we demonstrate that budding yeast condensin is recruited to pericentromeres through a direct interaction between its Ycg1 subunit and the pericentromeric adaptor protein, shugoshin (Sgo1). We identify a Short Linear Motif (SLiM), termed CR1, within the C-terminal region of Sgo1 which inserts into a conserved pocket on Ycg1. Disruption of this interface abolishes the Sgo1-condensin interaction, prevents condensin recruitment to pericentromeres and results in defective sister kinetochore biorientation in mitosis. Similar motifs to CR1 are found in known and potential condensin binding partners and the Ycg1 binding pocket is broadly conserved, including in mammalian CAP-G proteins. Overall, we uncover the molecular mechanism that targets condensin to define a specialized chromosomal domain.

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