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Single-cell analysis of the dLN-tumor OL clones following anti-CD4 mAb treatment

ABSTRACT: Single-cell analysis of the dLN-tumor OL clones following anti-CD4 mAb treatment

PROVIDER: PRJNA814222 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18278261.fastq.gz	Fastqsanger.gz
	SRR18278262_1.fastq.gz	Fastqsanger.gz
	SRR18278262_2.fastq.gz	Fastqsanger.gz
	SRR18278263_1.fastq.gz	Fastqsanger.gz
	SRR18278263_2.fastq.gz	Fastqsanger.gz

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Single-cell analysis of the dLN-tumor OL clones following anti-CD4 mAb treatment

Project description:The repertoire of tumor-infiltrating T cells is a novel perspective to characterize effective anti-tumor T cell responses. Previous studies reported that the oligoclonal expansion in tumor T-cell repertoire is associated with anti-tumor effects. However, the contribution of the expansion of diverse T-cell clones remained unclear. We demonstrated that the polyclonal fraction of tumor-reactive T-cell repertoire consisting of relatively minor clones, increased in tumor-bearing mice treated with anti-PD-L1 or anti-CD4 monoclonal antibodies (mAbs), while the oligoclonal fraction consisting of major clones was unchanged. Moreover, the polyclonal fraction was enriched with progenitor exhausted T cells, essential for a durable anti-tumor response, and more dependent on CCR7+ migratory dendritic cells, responsible for priming tumor-reactive T cells in the tumor-draining lymph node (dLN). These results proposed that the expansion of diverse tumor-reactive clones, “clonal spreading,” is an important mechanism by which anti-PD-L1 and anti-CD4 treatments exert robust and durable anti-tumor T-cell responses.

2023-05-11 | GSE198209 | GEO

TCR repertoire analysis revealed the mobilization of novel CD8+ T cell clones into Cancer-Immunity Cycle following anti-CD4 antibody administration

Project description:Depletion of CD4+ cells by anti-CD4 monoclonal antibody (anti-CD4 mAb) induces expansion of tumor-reactive CD8+ T cells and exhibits strong antitumor effects in several murine tumor models. However, whether the anti-CD4 mAb treatment activates particular or a broad variety of tumor-reactive CD8+ T cell clones is not answered. To investigate the changes of TCR repertoire induced by the anti-CD4 mAb treatment, we performed unbiased high throughput TCR sequencing in a B16F10 mouse subcutaneous melanoma model.

2019-02-07 | GSE115425 | GEO

Bulk TCRseq analysis on CD8+ T cells in wild type mice following anti-CD4 and anti-PDL1 mAb treatment

2023-05-11 | GSE198208 | GEO

Bulk TCRseq analysis on CD8+ T cells in FSCN1-KI bone marrow chimera mice following anti-CD4 and anti-PDL1 mAb treatment

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Caldicellulosiruptor owensensis OL strain:OL

Project description:Caldicellulosiruptor owensensis OL genome sequencing

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Expression data from OL and OC treated B16F10 cells

Project description:Oleocanthal (OL) and Oleacein (OC) are enriched components in Olive (oil and leaves). However, their effects on skin have not been widely studied compared to Oleuropein (OP). Here, we perfomed global gene expression profiling to screen the potential effects of OL and OC on the skin. Results showed that OL and OC have effects on skin development and keratinocyte differentiation by upregulation of key markers. Furthermore, OL and OC were effectively downregulated several melanogenic genes and it suggests that OL and OC can have a potential effect for pigmentation. B16F10 were murine melanoma cells line, treated with 5 µM of OL and OC for 24 hours. Microarray gene expression profiling was conducted technical replicates.

2024-05-01 | GSE237508 | GEO

Tepidanaerobacter syntrophicus strain:OL

Project description:Tepidanaerobacter syntrophicus strain OL

| PRJDB14931 | ENA

RNA sequencing of human regulatory T cell clones, both Faecalibacterium prausnitzii-reactive DP8a (CD4,CD8alphalow) Tregs and (CD4,CD25high,CD127low) Foxp3 Tregs obtained from the colon lamina propria and from the blood.

Project description:We generated human DP8a Treg (CD4, CD8alow, CCR6+, CXCR6+, F. praunsitzii-reactive) and FoxP3 Treg (CD4, CD25high, CD127low) clones from healthy colon lamina propria-derived lymphocytes (LPL) (from colorectal cancer patients) and from blood lymphocytes from healthy volunteers). Through DEseq2 analysis) we identified activation-induced genes in these clones and compared gene expression between resting and activated DP8a and Foxp3 clones.

2022-11-09 | GSE197906 | GEO

NKT, CD1d-aGC+ Va24-, and CD4 T cell clones from human peripheral blood

Project description:Microarray analysis was performed to determine the transcriptional profiles of NKT, CD1d-aGC+ Va24-, and CD4 T cells. Clones of NKT, CD1d-aGC+ Va24-, and CD4 T cells were generated using peripheral blood drawn from two healthy individuals. RNA was extracted from cells that had been either rested (18 days post-stimulation) or stimulated (3 days post-stimulation).

2011-04-22 | E-GEOD-28726 | biostudies-arrayexpress

Integration of Metabolomics and Transcriptomics Reveals Changes in MRSA Exposed to Terpinen-4-ol

Project description:Staphylococcus aureus (S. aureus) is a gram-positive bacterium that causes a wide range of diseases. Terpinen-4-ol is a monoterpene component contained in most plant essential oils with good antibacterial activity. In this study, terpinen-4-ol effectively inhibited 13 strains of S. aureus, and effectively inhibited the biofilm of MRSA. Metabolomics and transcriptomics were used to elucidate changes in MRSA cells exposed to terpinen-4-ol. Terpinen-4-ol significantly changed (greater than a 2- or less than a 2-fold change) the expression of 304 genes and the level of 847 metabolites (greater than a 1.5- or less than a 0.67-fold change) in MRSA. The levels of genes and metabolites related to the valine, leucine and isoleucine biosynthesis metabolism pathway, the purine and pyrimidine metabolism pathway, energy metabolism and β-lactam resistance were dramatically changed in biofilms exposed to terpinen-4-ol. To the best of our knowledge, this research is the first to report the metabolite and expression profiles of MRSA exposed to terpinen-4-ol.

2020-09-09 | GSE157638 | GEO

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