Project description:In the present study, we employed the high-throughput sequencing technology to profile miRNAs in the inner and outer pericarp of Actinidia chinensis cv. Hongyang. After sequencing and cleaning, the numbers of clean reads generated from these four libraries were 18,203,332, 9,356,430, 11,006,231 and 11,314,375, respectively. Approximately 93.62%, 93.67%, 92.75% and 93.28% clean reads were respectively mapped to the kiwifruit genome with perfect matches. Subsequently, differentially expressed genes were compared between the inner and outer pericarps. Significant differences in both up- and down-regulated genes were identified in inner and outer percarps by comparing expression levels. The results showed that there were significantly 450 up-regulated and 416 down-regulated DEGs in inner pericarp as compared to the outer pericarp.
Project description:Transcriptome analysis of Leaves at Different Developmental Stages in Hongyang Kiwifruit (Actinidia chinensis var. chinensis) (PRJCA039338)
Project description:Actinidia chinensis (kiwifruit) are economically important fruit-bearing woody perennial vines, but with a long juvenile phase which slows plant breeding efforts. LEAFY (LFY) encodes a plant specific transcription factor central to the reproductive pathway in many plants, and its overexpression can accelerate flowering including in some trees. We identified two kiwifruit LFY (AcLFY) genes, expressed in actively-growing apical buds. Overexpression of AcLFY1 or AcLFY2 promoted flowering in Arabidopsis, but did not accelerate flowering in kiwifruit. Instead, branching at the lower nodes and changes in leaf morphology were observed. CRISPR-Cas9 targeted mutagenesis of AcLFY1 and/or AcLFY2 in a fast-flowering hermaphrodite kiwifruit background generated single and double mutants. The mutants flowered at the same time as control lines. Single mutants developed normal flowers, but double knockout mutations had severe effects on floral patterning and floral organ development. Petals and stamens were strongly affected, impacting on self-pollination, fruit size and shape. RNA-seq showed the two AcLFY genes were differentially regulated by the expression of a floral repressor CENTRORADIALIS gene AcCEN4. These results suggest that the AcLFY genes control plant architecture and contribute together to floral organ development and floral patterning which has implications for successful pollination and fruit set in kiwifruit.
