Project description:Cannabis sativa L. is a dioecious, annual herbaceous crop (2n = 20) known for its diverse applications across textile, food, cosmetic, and medicinal industries. Recent increases in global temperatures have raised concerns about the impact of heat stress on hemp physiology. Although previous studies have demonstrated reductions in photosynthetic efficiency and cannabinoid levels under heat stress, transcriptome-wide insights into molecular responses remain limited. In this study, we performed a genome-wide transcriptomic analysis to investigate the molecular mechanisms of heat stress response in C. sativa cv. Pink Pepper. This dataset provides a high-resolution transcriptomic resource to explore thermotolerance in C. sativa and contributes to a better understanding of its molecular adaptation to heat stress. The findings can aid future breeding and biotechnology efforts aimed at enhancing stress resilience in hemp.
Project description:Low temperature is a major abiotic stress factor that promotes oxidative stress and disrupts cellular homeostasis, ultimately impairing plant physiological functions. This study investigated the effects of exogenous putrescine treatment on the cold stress response of Cannabis sativa L. This in silico transcriptome analysis demonstrated that putrescine mediates cold stress tolerance in C. sativa, providing valuable insight for future in vitro validation studies and a foundational framework for the development of cold-resistant hemp cultivars.
Project description:Low temperature is a major abiotic stress factor that promotes oxidative stress and disrupts cellular homeostasis, ultimately impairing plant physiological functions. This study investigated the effects of exogenous putrescine treatment on the cold stress response of Cannabis sativa L. This in silico transcriptome analysis demonstrated that putrescine mediates cold stress tolerance in C. sativa, providing valuable insight for future in vitro validation studies and a foundational framework for the development of cold-resistant hemp cultivars.
Project description:Heavy metals are environmental pollutants significantly affecting soil quality and, consequently, plant growth. Cadmium (Cd) is a highly toxic element primarily introduced into the environment through anthropogenic activities. The impact of Cd on plants is particularly severe, as it is taken up by roots and translocated to aerial tissues, where it accumulates and disrupts cellular metabolism. Zinc (Zn), conversely, is an essential micronutrient required for several enzymatic functions, including those involved in DNA synthesis, protein production, and oxidative stress regulation. However, excessive Zn levels in soils, often resulting from industrial effluents, mining, and improper disposal of Zn-containing products, can lead to toxic effects on plants. Hemp (Cannabis sativa L.), a multi-purpose crop grown for its long and strong bast fibres, nutrient-rich seeds, and medicinal compounds has gained attention for its ability to tolerate and accumulate heavy metals, including Cd and Zn. These unique characteristics position hemp as a promising candidate for phytoremediation. In this study, the impact of Cd and Zn on bast fibre formation was evaluated on young hemp plants, by focusing on the hypocotyl, a structure shown to be a suitable model to address questions related to fibre formation. In particular, the impact of Cd and Zn in the presence and absence of the beneficial quasi-essential metalloid silicon (Si) was studied by merging optical and high-resolution microscopy with -omics.
Project description:Even if a large amount of high-throughput functional genomic data exists, most researchers feature a strong background in molecular biology but lack advanced bioinformatics skills. In this work, publicly available gene expression datasets have been analyzed giving rise to a total of 40,224 gene expression profiles within different Cannabis tissues/developmental stages. The resource here proposed will provide researchers with a starting point for future investigations of Cannabis sativa.
Project description:Cannabis sativa L., which has been reclassified as an agronomic crop, has experienced an increase in cultivation. Its interactions with a variety of environmental stressors have been extensively studied. However, the mechanisms of recovery through fungal associations remain underexplored. Trichoderma hamatum, known for its role as a biological agent, enhances plant growth and provides antagonistic defense against pathogenic microbes. This meta-dataset aims to investigate whether Th can enhance drought resistance in a Cannabis plants.
Project description:Cannabis sativa, a member of the Cannabaceae family, is predominantly a dioecious species, producing male and female flowers on separate individuals. Although sex determination follows a heterogametic XY system, considerable plasticity in sex expression is evident. To investigate the gene regulatory networks underlying male and female flower development, we performed transcriptomic profiling of shoot apices from vegetative male and female inflorescences at one and three weeks following floral induction. Our analysis identified key MADS-box genes associated with floral organ specification and revealed that cannabis flower development largely conforms to the canonical ABCDE model
Project description:Drought stress is the main environmental factor influencing hemp growth and yield. However, little is known about the response mechanism of hemp to drought stress. A total of 44.10 M tags and 8.91G bases were sequenced in the control hemp (CK) and drought stress hemp (DS) libraries. A total of 1292 differentially expressed genes (DEGs), including 883 up-regulated genes and 409 down-regulated genes, were identified. These results may contribute toward improving our understanding about the drought stress regulatory mechanism of hemp, and improving its drought tolerance ability. 3' tag-based DGE libraries were generated to exam the differentially expressed gene between drought-stressed and well-watered hemp
Project description:Drought stress is the main environmental factor influencing hemp growth and yield. However, little is known about the response mechanism of hemp to drought stress. A total of 44.10 M tags and 8.91G bases were sequenced in the control hemp (CK) and drought stress hemp (DS) libraries. A total of 1292 differentially expressed genes (DEGs), including 883 up-regulated genes and 409 down-regulated genes, were identified. These results may contribute toward improving our understanding about the drought stress regulatory mechanism of hemp, and improving its drought tolerance ability.