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Hemitrygon akajei

ABSTRACT: Hemitrygon akajei overview

PROVIDER: PRJNA816214 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	DRR215720_1.fastq.gz	Fastqsanger.gz
	DRR215720_2.fastq.gz	Fastqsanger.gz
	DRR215721_1.fastq.gz	Fastqsanger.gz
	DRR215721_2.fastq.gz	Fastqsanger.gz

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Hemitrygon akajei

Project description:Differential expression analysis on the kidney of Japanese red stingray (Hemitrygon akajei)

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Hemitrygon akajei

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Hemitrygon akajei

Project description:RNA-seq analysis of the kidneys of Japanese red stingray (Hemitrygon akajei)

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Molecular and morphological investigations on the renal mechanisms enabling euryhalinity of red stingray <i>Hemitrygon akajei</i>.

Project description:Most cartilaginous fishes live in seawater (SW), but a few exceptional elasmobranchs (sharks and rays) are euryhaline and can acclimate to freshwater (FW) environments. The plasma of elasmobranchs is high in NaCl and urea concentrations, which constrains osmotic water loss. However, these euryhaline elasmobranchs maintain high levels of plasma NaCl and urea even when acclimating to low salinity, resulting in a strong osmotic gradient from external environment to body fluid. The kidney consequently produces a large volume of dilute urine to cope with the water influx. In the present study, we investigated the molecular mechanisms of dilute urine production in the kidney of Japanese red stingray, Hemitrygon akajei, transferred from SW to low-salinity environments. We showed that red stingray maintained high plasma NaCl and urea levels by reabsorbing more osmolytes in the kidney when transferred to low salinity. RNA-seq and qPCR analyses were conducted to identify genes involved in NaCl and urea reabsorption under the low-salinity conditions, and the upregulated gene expressions of Na+-K+-Cl- cotransporter 2 (nkcc2) and Na+/K+-ATPase (nka) were found in the FW-acclimated individuals. These upregulations occurred in the early distal tubule (EDT) in the bundle zone of the kidney, which coils around the proximal and collecting tubules to form the highly convoluted structure of batoid nephron. Considering the previously proposed model for urea reabsorption, the upregulation of nkcc2 and nka not only causes the reabsorption of NaCl in the EDT, but potentially also supports enhanced urea reabsorption and eventually the production of dilute urine in FW-acclimated individuals. We propose advantageous characteristics of the batoid-type nephron that facilitate acclimation to a wide range of salinities, which might have allowed the batoids to expand their habitats.

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Hemitrygon akajei isolate:Umi21f | breed:wild

Project description:Comparative analysis of batoid genomes

| PRJNA1206076 | ENA

Hemitrygon bennettii

Project description:Dasyatis bennetti overview

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Ghrelin-like peptide with fatty acid modification and O-glycosylation in the red stingray, Dasyatis akajei.

Project description:BackgroundGhrelin (GRLN) is now known to be an appetite-stimulating and growth hormone (GH)-releasing peptide that is predominantly synthesized and secreted from the stomachs of various vertebrate species from fish to mammals. Here, we report a GRLN-like peptide (GRLN-LP) in a cartilaginous fish, the red stingray, Dasyatis akajei.ResultsThe purified peptide contains 16 amino acids (GVSFHPQPRS10TSKPSA), and the serine residue at position 3 is modified by n-octanoic acid. The modification is the characteristic of GRLN. The six N-terminal amino acid residues (GVSFHP) were identical to another elasmobranch shark GRLN-LP that was recently identified although it had low identity with other GRLN peptides. Therefore, we designated this peptide stingray GRLN-LP. Uniquely, stingray GRLN-LP was O-glycosylated with mucin-type glycan chains [N-acetyl hexosamine (HexNAc)3 hexose(Hex)2] at threonine at position 11 (Thr-11) or both serine at position 10 (Ser-10) and Thr-11. Removal of the glycan structure by O-glycanase made the in vitro activity of stingray GRLN-LP decreased when it was evaluated by the increase in intracellular Ca2+ concentrations using a rat GHS-R1a-expressing cell line, suggesting that the glycan structure plays an important role for maintaining the activity of stingray GRLN-LP.ConclusionsThis study reveals the structural diversity of GRLN and GRLN-LP in vertebrates.

| S-EPMC2803784 | biostudies-literature

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Project description:An overview of small RNAs sequences existing in seed development and contrasted with vegetative tissues of the soybean

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Project description:A genomic overview of in vivo binding of a transcription factor Snail in the ascidian early gastrula embryo.

2010-03-09 | GSE17691 | GEO