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Generation of human kidney organoids from urine cells (ATAC-seq)

ABSTRACT: Generation of human kidney organoids from urine cells (ATAC-seq)

PROVIDER: PRJNA817168 | ENA |

REPOSITORIES: ENA

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Generation of human kidney organoids from urine cells (ATAC-seq)

Project description:Kidney organoids are ideal models to study the complex process of human kidney development. Here we report the generation of functional kidney organoids by reprogramming human urine epithelial cells (hUCs). RNA-seq and ATAC-seq revealed the three-stage process of the 2D U-iRO induction. Single cell RNA-seq further reveals the cell types in 2D and 3D organoids, 2D U-iRO dominated with mesenchyme and 3D U-iRO with tubule.

2022-10-18 | GSE198838 | GEO

Generation of human kidney organoids from urine cells

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Generation of human kidney organoids from urine cells

Project description:Generation of human kidney organoids from urine cells

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Generation of human kidney organoids from urine cells (RNA-seq)

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Generation of intestinal organoids from human urine cells

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Single cell ATAC-seq of iPSC-derived Kidney Organoids treated with TGF-Beta 1 and GSK343 compared with vehicle treated control

Project description:This study used snATAC-seq to profile Chromatin accessibility in 26 day-old iPSC-derived kidney organoids, treated with TGFB1, the EzH2 inhibitor GSK343, a combination of both or a vehicle control for 48 hours (days 24-26) before harvesting. 2 organoids per condition were pooled and dissociated using a cold-active protease. Nuclei were extracted and profiled using the 10X Genomics Single-cell ATAC reagent kit v1.1. Libraries were sequenced using paired-end reads on an Illumina NovaSeq 6000. Initial processing was performed using CellRanger ATAC v1.2.0 (10X Genomics).

2023-04-30 | E-MTAB-11139 | biostudies-arrayexpress

Single cell RNA-seq on kidney allograft urine samples

Project description:We performed 10x Genomics single-cell RNA sequencing (scRNA-seq) on 12 urine samples from kidney transplant recipients. These urine samples contain a mixture of donor-derived kidney cells and recipient-derived immune and bladder cells. To our knowledge, this is the first study to apply single-cell transcriptomics to urine samples from kidney allograft recipients.

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