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The role of the cap methyltransferase RNMT in mouse neuron development and neuronal acitvity

ABSTRACT: The role of the cap methyltransferase RNMT in mouse neuron development and neuronal acitvity

PROVIDER: PRJNA817583 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18369717_1.fastq.gz	Fastqsanger.gz
	SRR18369717_2.fastq.gz	Fastqsanger.gz
	SRR18369718_1.fastq.gz	Fastqsanger.gz
	SRR18369718_2.fastq.gz	Fastqsanger.gz
	SRR18369719_1.fastq.gz	Fastqsanger.gz

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mRNA cap methyltransferase, RNMT-RAM, promotes RNA pol II transcription

Project description:mRNA cap addition occurs early during RNA pol II transcription, facilitating pre-mRNA processing and translation. We report that the mammalian mRNA cap methyltransferase, RNMT-RAM, promotes RNA pol II transcription, independently of mRNA capping and translation. In cells, sub-lethal suppression of RNMT-RAM reduces RNA pol II occupancy, net mRNA synthesis and pre-mRNA levels. Conversely, expression of RNMT-RAM increases transcription independently of cap methyltransferase activity. In isolated nuclei, recombinant RNMT-RAM stimulates transcriptional output; this requires the RAM RNA-binding domain. RNMT-RAM interacts with nascent transcripts along their entire length and with transcription associated factors including RNA pol II subunits, SPT4, SPT6 and PAFc. Suppression of RNMT-RAM inhibits transcriptional markers including histone H2B K120 ubiquitination, H3 K4 and K36 methylation, RNA pol II S5 and S2 phosphorylation and PAFc recruitment. These findings suggest that multiple interactions between RNMT-RAM, RNA pol II factors and RNA along the transcription unit stimulate transcription.

2018-05-01 | GSE87767 | GEO

The role of the cap methyltransferase RNMT in mouse neuron development and neuronal acitvity

Project description:The roles of the cap methlytransferase RNMT were investigated in both control neurons and those stimulated using the membrane depolarising agent KCl.

2025-03-18 | GSE198964 | GEO

RNMT regulates the naïve CD4 T cell transcriptome

Project description:We investigated the role of RNMT in T cells using an Rnmt conditional knockout mouse model. We report that the mRNA cap methyltransferase, RNMT, supports naïve T cell survival and activation-induced proliferation. We demonstrate that RNMT has gene-specific impacts in T cells, selectively regulating expression of terminal polypyrimidine tract (TOP) mRNAs which are targets of the m7G-cap binding protein LARP1. These RNAseq experiments investigate the effect of Rnmt cKO on the naïve CD4 T cell transcriptome.

2021-05-13 | GSE160267 | GEO

RNMT regulates the activated CD4 T cell transcriptome and translatome [Ribo-Seq]

Project description:We investigated the role of RNMT in T cells using an Rnmt conditional knockout mouse model. We report that the mRNA cap methyltransferase, RNMT, supports naïve T cell survival and activation-induced proliferation. We demonstrate that RNMT has gene-specific impacts in T cells, selectively regulating expression of terminal polypyrimidine tract (TOP) mRNAs which are targets of the m7G-cap binding protein LARP1. These ribosome footprinting experiments investigate the effect of Rnmt cKO on the activated CD4 T cell transcriptome and translatome.

2021-05-13 | GSE160326 | GEO

Psuedouridine sequencing rRNA in Rnmt cKO CD4 T cells

Project description:We investigated the role of RNMT in T cells using an Rnmt conditional knockout mouse model. We report that the mRNA cap methyltransferase, RNMT, supports naïve T cell survival and activation-induced proliferation. We demonstrate that RNMT has gene-specific impacts in T cells, selectively regulating expression of terminal polypyrimidine tract (TOP) mRNAs and small RNAs including snoRNAs. To deterine if had any effect on ribosomal RNA modification we carried out pseudoridine sequencing.

2021-05-13 | GSE165790 | GEO

Rnmt cKO vs control activated CD4 T cell ribo Mega-SEC

Project description:We aimed to identify how loss of the cap methyltransferase RNMT affected the ribosomes and ribosome-associated proteins in activated CD4 T cells.

2021-05-13 | PXD023832 | Pride

Investigation of the RNMT/RAM RNA interactome in mouse embryonic stem cells

Project description:Pilot investigation to determine the endogenouse RNA-binding targets of the cap methlytransferase complex RNMT/RAM in mouse ES cells

2025-03-11 | GSE198543 | GEO

Investigation of the RNMT/RAM RNA interactome in mouse embryonic stem cells [Brain_seCLIP]

Project description:Pilot investigation to determine the endogenouse RNA-binding targets of the cap methlytransferase complex RNMT/RAM in mouse ES cells

2025-03-14 | GSE198579 | GEO

Investigation of the RNMT/RAM RNA interactome in mouse embryonic stem cells [RIP-Seq]

Project description:Pilot investigation to determine the endogenouse RNA-binding targets of the cap methlytransferase complex RNMT/RAM in mouse ES cells

2025-03-18 | GSE198965 | GEO

Identification of LARP1 targets in naive CD4 T cells [eCLIP]

Project description:We investigated the role of RNMT in T cells using an Rnmt conditional knockout mouse model. We report that the mRNA cap methyltransferase, RNMT, supports naïve T cell survival and activation-induced proliferation. We demonstrate that RNMT has gene-specific impacts in T cells, selectively regulating expression of terminal polypyrimidine tract (TOP) mRNAs which are targets of the m7G-cap binding protein LARP1. These LARP1 eCLIP experiments determine the RNA binding sites of LARP1 in naive CD4 T cells from Rnmt cKO and control mice.

2021-05-13 | GSE160325 | GEO

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