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Single-cell sequencing of wt and Rbx1-deficient Treg cells from mice

ABSTRACT: Single-cell sequencing of wt and Rbx1-deficient Treg cells from mice

PROVIDER: PRJNA819178 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18458697_1.fastq.gz	Fastqsanger.gz
	SRR18458697_2.fastq.gz	Fastqsanger.gz
	SRR18458698_1.fastq.gz	Fastqsanger.gz
	SRR18458698_2.fastq.gz	Fastqsanger.gz

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Single-cell sequencing of wt and Rbx1-deficient Treg cells from mice

Project description:Single-cell RNA sequencing (scRNA-seq) was utilized to unbiasedly dissect the cellular heterogeneity of wt vs. Rbx1-deficient CD4+YFP+ Treg cells from the peripheral lymph nodes of inflammation-free female Foxp3cre/wt and Foxp3cre/wt;Rbx1fl/fl mice.

2022-03-26 | GSE199289 | GEO

Transcriptome data of wt, Rbx1- or Ube2m- deficient Treg cells from mice

Project description:Cullin-RING Ligases (CRLs), upon activation by neddylation, play the key roles in regulating many biological processes. However, how CRL-neddylation regulates the function of Treg cells remains elusive. Here we show that mice with Treg-specific deletion of Rbx1, a RING component of CRLs required for its activity, developed an early-onset fetal inflammatory disorders and death at day ~25 after birth with disrupted homeostasis and impaired suppressive functions of Treg cells. Specifically, Rbx1 is essential for maintenance of the effector subpopulations in Treg cells, and regulates several inflammatory pathways. Similar phenotypes were seen in mice with deletion of Ube2m, a neddylation E2, in Treg cells, but with much lesser severity. Interestingly, Treg-specific deletion of Rbx2/Sag or Ube2f, the family member of Rbx1 or Ube2m, respectively, had no obvious phenotype. Thus, the Ube2m-Rbx1 axis is required for the maintenance of homeostasis and functions of Treg cells; and Rbx1 has Ube2m-independent roles in the fitness of Treg cells, suggesting a layer of complexity in neddylation activation of CRLs. We use the tranpritome dara to show the mechanism of the function of Rbx1 and Ube2m in Treg cells, also research the relationship of Rbx1 and Ube2m in Treg cells.

2021-12-28 | GSE192664 | GEO

Transcriptome data of wt, Rbx1- or Ube2m- deficient Treg cells from mice

Project description:Transcriptome data of wt, Rbx1- or Ube2m- deficient Treg cells from mice

| PRJNA792577 | ENA

Transcriptome data of Foxp3-Cre, Rbx1&Sag-deficient Treg cells from mice

Project description:Neddylation is necessary for activation of Cullin-RING Ligases (CRLs) which degrade various immune regulatory proteins. Our recent study showed that while depletion of neddylation E2-E3 pair Ube2f-Sag in regulatory T cells (Treg cells) had no obvious phenotype, the same depletion of either Ube2m or Rbx1 caused inflammation disorders with different severity. Whether these E2s or E3s compensate each other in functional regulations of Treg cells is, however, previously unknown. In this report, we generated Foxp3Cre;Ube2mfl/fl;Ube2ffl/fl or Foxp3Cre;Rbx1fl/fl;Sagfl/fl double null mice by simultaneously deletion of both neddylation E2s or E3s in Treg cells, respectively. Remarkably, Ube2m&Ube2f double null mice developed much severe autoimmune phenotypes than Ube2m-null mice, indicating Ube2m significantly compensates Ube2f in Treg cells. The minor worsen autoimmune phenotypes seen at very early stage in Rbx1&Sag double null than Rbx1-null mice, is likely due to already severe phenotypes of the later, indicating a minor compensation of Rbx1 for Sag. The RNA profiling-based analyses revealed that up- and down-regulations of few signaling pathways in Treg cells are associated with the severity of autoimmune phenotypes. Finally, severer inflammation phenotypes seen in mice with double E3-null than with double E2-null Treg cells indicate a neddylation-independent mechanism of two E3s, also known to serve as the RING component of CRLs in regulation of Treg cell fitness.

2023-08-12 | GSE237498 | GEO

Transcriptome data of Foxp3-Cre, Rbx1&Sag-deficient Treg cells from mice

Project description:Transcriptome data of Foxp3-Cre, Rbx1&Sag-deficient Treg cells from mice

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Transcriptome data of Foxp3-Cre, Ube2m&Ube2f-deficient Treg cells and Foxp3-Cre, Rbx1&Sag-deficient Treg cells from mice

Project description:Transcriptome data of Foxp3-Cre, Ube2m&Ube2f-deficient Treg cells and Foxp3-Cre, Rbx1&Sag-deficient Treg cells from mice

| PRJNA995529 | ENA

Single-cell RNA-Seq analysis of WT and Bach2-deficient Foxp3+ Treg cells

Project description:These are the FastQ files used for scRNA-Seq analysis of WT and Bach2-deficient splenic Foxp3+ Treg cells

2020-05-12 | GSE128175 | GEO

Gene expression profile at single cell level of Treg cells (CD4+CD25+) from healthy Mycfl/flFoxp3Cre/WT female mice, in which Myc-sufficient (WT) and Myc-deficient (KO) Treg cells co-exist

Project description:We performed single cell transcriptome analysis of Treg cells (CD4+CD25+) from healthy Mycfl/flFoxp3Cre/WT female mice, in which Myc-sufficient (WT) and Myc-deficient (KO) Treg cells co-exist due to random inactivation of the X chromosome that harbors the Foxp3 gene.

2022-07-17 | GSE208252 | GEO

Transcriptome data of Foxp3-Cre, Ube2m&Ube2f-deficient Treg cells from mice

2023-08-12 | GSE237497 | GEO

Expression data on Treg cells isolated from wildtype mice and miR-125a deficient mice

Project description:We found miR-125a was a key regulator that stabilizes the commitment and immunoregulatory capacity of Treg cells.To gain insights into the general functional features of miR-125a-deficient Treg cells, we performed a genome-wide gene array analysis on Treg population isolated from the spleens of 6 to 8-week-old miR-125a-deficient and WT mice We sorted CD4+CD25hi Treg population from the spleens of 6 to 8-week-old miR-125a-deficient and their littermate WT mice. Cells were collected and total RNA was extracted for Affymetrix GeneChip®Mouse Genome 430 2.0 Array

2015-05-08 | E-GEOD-66608 | biostudies-arrayexpress

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