Project description:PD-1+/- Th cells were sorted by flow cytometry C57BL/6 mice were infected with P. yoelii. The differentially expressed genes of PD-1+/- Th cells from the spleen of the infected mice were acquired by microarray
Project description:Primary outcome(s): Correlation between PD-L1 expression in immune cells and soluble PD-L1 value. Correlation between PD-L1 expression in immune cells or soluble PD-L1 value and PD-L1 expression in tumor tissue. Characterstics of PD-L1 expression in immune cells or soluble PD-L1 value in each gastrointestinal cancer type.
Project description:Human Th, Treg and Th and Treg together can induce monocytes differentiate into different myeloid cell population. We were trying to compare the transcriptome of these populations. We used microarrays to detail transcriptome of Th and Treg induced regulatory DCs.
Project description:We compared gene expression profiles of Th cells, macrophages and monocytes isolated from the inflamed colon of colitis induced by the transfer of WT versus Tbx21-/- Th cells in Rag1-/- recipients.
Project description:LC-MS/MS targeted files submitted as support material for the paper: Quantitative mass spectrometry analysis of PD-L1 protein expression, N-glycosylation and expression stoichiometry with PD-1 and PD-L2 in human melanoma.