Project description:The male sterility of a wheat thermosensitive genic male sterile (TGMS) line is strictly controlled by temperature. We used microarrays to identify genes that play pivotal roles in anthers during cold-stress hypersensitivity. The expression of genes in response to different temperature treatment were analyzed for anthers of BS366.
Project description:The male sterility of a wheat thermosensitive genic male sterile (TGMS) line is strictly controlled by temperature. We used microarrays to identify genes that play pivotal roles in anthers during cold-stress hypersensitivity.
Project description:Bread wheat (Triticum aestivum L., cv. Fielder) plants were grown under iron (Fe) deficient hydroponic conditions to analyise transcriptomic changes in leaf and root tissue.
Project description:Wheat (Triticum aestivum), one of the most important cereal crops, it provides many kinds of food for humans and animals, in this study, we performed the first comprehensive phosphoproteome analysis to study the regulatory mechanism of bread quality formation under different nitrogen fertilizer. Totally, 2470 phosphotides, represented 1372 proteins were identified in our study. and 411 proteins showed significant differences.
Project description:MS-1 data of two varieties of wheat (Triticum sp.) under control and pathogen inoculated condition. C_NEG and I_NEG files represent MS-1 data in negative ion mode under Control and pathogen Inoculated conditions respectively. Similarly, C_POS and I_POS files represent MS-1 data in positive ion mode under Control and pathogen Inoculated conditions respectively.
Project description:Nitrogen (N) is an essential nutrient element for crop productivity. Unfortunately, the nitrogen use efficiency (NUE) of crop plants gradually decreases with the increase of the nitrogen application rate. Nevertheless, little has been known about the molecular mechanisms of differences in NUE among genotypes of wheat. The use of near-isogenic lines (NILs) in transcriptome analysis can reduce genetic background noise. In this study, we used RNA-Sequencing (RNA-Seq) to compare the transcriptome profiling in NILs (1Y, high-NUE, and 1W, low-NUE) under normal nitrogen conditions. The results showed that 7,023 DEGs (4,738 up-regulated and 2,285 down-regulated) were identified in the 1Y vs 1W comparison. The responses of 1Y and 1W to normal nitrogen differed significantly in the transcriptional regulation mechanisms. Several genes belonging to the GS and GOGAT gene families were up-regulated in 1Y compared with 1W, and the enhanced carbon metabolism might lead 1Y to produce more C skeletons, metabolic energy, and reductants for nitrogen metabolism. A subset of transcription factors (TFs) family members such as ERF, WRKY, NAC, and MYB were also identified. Collectively, these identified candidate genes provided new information for a further understanding of the genotypic difference in NUE