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RNA-seq experiment of wild-type (WT) and pif4 mutant seedlings grown for 3 days in darkness and exposed or not to heat stress for 3 hours.

ABSTRACT: RNA-seq experiment of wild-type (WT) and pif4 mutant seedlings grown for 3 days in darkness and exposed or not to heat stress for 3 hours.

PROVIDER: PRJNA823735 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18649037.fastq.gz	Fastqsanger.gz
	SRR18649038.fastq.gz	Fastqsanger.gz
	SRR18649039.fastq.gz	Fastqsanger.gz
	SRR18649040.fastq.gz	Fastqsanger.gz
	SRR18649041.fastq.gz	Fastqsanger.gz

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RNA-seq experiment of wild-type (WT) and pif4 mutant seedlings grown for 3 days in darkness and exposed or not to heat stress for 3 hours.

Project description:Transcriptome profiles of wild-type (WT), pif4-101, and transgenic plants expressing the coding region of PIF4 under the control of its endogenous promoter (H71 and H76; pif4-101 mutant background) grown for 3 days in the dark at 22ºC and then, for 3 additional hours, exposed or not to heat stress (37ºC).

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Interaction between BZR1 and PIF4 integrates brassinosteroid and environmental responses [ChIP-seq]

Project description:Plant growth is coordinately regulated by environmental and hormonal signals. Brassinosteroid (BR) plays essential roles in growth regulation by light and temperature, but the interactions between BR and these environmental signals remain poorly understood at the molecular level. Here, we show that direct interaction between the dark- and heat-activated transcription factor phytochrome-interacting factor4 (PIF4) and the BR-activated transcription factor BZR1 integrates the hormonal and environmental signals. BZR1 and PIF4 interact with each other in vitro and in vivo, bind to nearly two thousand common target genes, and synergistically regulate many of these target genes, including the PRE family HLH factors required for promoting cell elongation. Genetic analysis indicates that BZR1 and PIFs are interdependent in promoting cell elongation in response to BR, darkness, or heat. These results show that the BZR1-PIF4 interaction controls a core transcription network, allowing plant growth co-regulation by the steroid and environmental signals. Genome-wide identification of PIF4 binding sites in etiolated Arabidopsis seedlings.

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Interaction between BZR1 and PIF4 integrates brassinosteroid and environmental responses [RNA-seq]

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Interaction between BZR1 and PIF4 integrates brassinosteroid and environmental responses [ChIP-seq]

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Interaction between BZR1 and PIF4 integrates brassinosteroid and environmental responses [RNA-seq]

Project description:Plant growth is coordinately regulated by environmental and hormonal signals. Brassinosteroid (BR) plays essential roles in growth regulation by light and temperature, but the interactions between BR and these environmental signals remain poorly understood at the molecular level. Here, we show that direct interaction between the dark- and heat-activated transcription factor phytochrome-interacting factor4 (PIF4) and the BR-activated transcription factor BZR1 integrates the hormonal and environmental signals. BZR1 and PIF4 interact with each other in vitro and in vivo, bind to nearly two thousand common target genes, and synergistically regulate many of these target genes, including the PRE family HLH factors required for promoting cell elongation. Genetic analysis indicates that BZR1 and PIFs are interdependent in promoting cell elongation in response to BR, darkness, or heat. These results show that the BZR1-PIF4 interaction controls a core transcription network, allowing plant growth co-regulation by the steroid and environmental signals. RNA-Seq for Col-0, bzr1-1D, pifq and pifq;bzr1-1D seedlings grown on BRZ-containing medium in the dark.

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Project description:To investigate the effect of the interaction between the MRG1/2 and PIF4, here we analysed differentially expressed genes compared to the mutant and wild-type by RNA-seq under different temperature. We performed gene expression profiling analysis using data obtained from RNA-seq of WT, mrg1 mrg2, pif4, and mrg1 mrg2 pif4 plants at 22degC and 28degC.

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Project description:Dark-grown seedlings exhibit skotomorphogenic development. Genetic and molecular evidence indicates that a quartet of Arabidopsis Phytochrome (phy)-Interacting bHLH Factors (PIF4, 3, 4 and 5) are critically necessary to maintaining this developmental state, and that light activation of phy induces a switch to photomorphogenic development by inducing rapid degradation of the PIFs. Here, using combined ChIP-seq and RNA-seq analyses, we have identified genes that are direct targets of PIF4 transcriptional regulation, and we provide evidence that the quartet collectively regulate these genes by shared, direct binding to the target promoters in promoting skotomorphogenesis. Three biological replicates data of PIF4-binding sites were collected by comparing the parallel ChIP samples from Myc-epitope-tagged-PIF4 (P1M) overexpressing transgenic seedlings and the wild-type (WT) control.

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The epidermis coordinates thermoresponsive growth through the phyB-PIF4-auxin pathway

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ChIP-seq for PIF4-HA plants under different temperatures.

Project description:To investigate the effect of the PIF4, we analysed PIF4 binding genes at 28℃ compared to 22℃.

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