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ABSTRACT: Probiotic supplementation in piglets

PROVIDER: PRJNA823879 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18652135_1.fastq.gz	Fastqsanger.gz
	SRR18652135_2.fastq.gz	Fastqsanger.gz
	SRR18652136_1.fastq.gz	Fastqsanger.gz
	SRR18652136_2.fastq.gz	Fastqsanger.gz
	SRR18652137_1.fastq.gz	Fastqsanger.gz

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Integrated transcriptomics and proteomics analysis reveals muscle metabolism effects of dietary Ulva lactuca and ulvan lyase supplementation in weaned piglets

Project description:Seaweeds, including the green Ulva lactuca, can potentially reduce competition between feed, food, and fuel. They can also contribute to the improved development of weaned piglets. However, their indigestible polysaccharides of the cell wall pose a challenge. This can be addressed through carbohydrase supplementation, such as the recombinant ulvan lyase. The objective of our study was to assess the muscle metabolism of weaned piglets fed with 7% U. lactuca and 0.01% ulvan lyase supplementation, using an integrated transcriptomics and proteomics approach. Feeding piglets with seaweed and enzyme supplementation resulted in reduced macronutrient availability, leading to protein degradation through the proteasome (PSMD2), with resulting amino acids being utilized as an energy source (GOT2, IDH3B). Moreover, mineral element accumulation (iodine and bromine) contributed to increased oxidative stress, evident from elevated levels of antioxidant proteins like catalase, as a response to maintain tissue homeostasis. The upregulation of the gene AQP7, associated with the osmotic stress response, further supports these findings. Consequently, an increase in chaperone activity, including HSP90, was required to repair damaged proteins. Our results suggest that enzymatic supplementation may exacerbate the effects observed from feeding U. lactuca alone, potentially due to side effects arising from cell wall degradation during digestion.

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Integrated transcriptomics and proteomics analysis reveals muscle metabolism effects of dietary Ulva lactuca and ulvan lyase supplementation in weaned piglets

Project description:Seaweeds, including the green Ulva lactuca, can potentially reduce competition between feed,food, and fuel. They can also contribute to the improved development of weaned piglets. However,their indigestible polysaccharides of the cell wall pose a challenge. This can be addressed throughcarbohydrase supplementation, such as the recombinant ulvan lyase. The objective of our study wasto assess the muscle metabolism of weaned piglets fed with 7% U. lactuca and 0.01% ulvan lyasesupplementation, using an integrated transcriptomics (RNA-seq) and proteomics (LC–MS) approach.Feeding piglets with seaweed and enzyme supplementation resulted in reduced macronutrientavailability, leading to protein degradation through the proteasome (PSMD2), with resulting aminoacids being utilized as an energy source (GOT2, IDH3B). Moreover, mineral element accumulationmay have contributed to increased oxidative stress, evident from elevated levels of antioxidantproteins like catalase, as a response to maintaining tissue homeostasis. The upregulation of the geneAQP7, associated with the osmotic stress response, further supports these findings. Consequently, anincrease in chaperone activity, including HSP90, was required to repair damaged proteins. Our resultssuggest that enzymatic supplementation may exacerbate the effects observed from feeding U. lactucaalone, potentially due to side effects of cell wall degradation during digestion.

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